Method for analyzing ferulaic acid butyric glyceride by high performance liquid chromatography

Abstract

The invention relates to a method for compartment analysis of ferulic acid glycerol monobutyralte through the high performance liquid chromatography, comprising the following: firstly, a step of pretreatment, during which, ferulic acids and absolute ethyl alcohols generate reflux heating reaction under the catalysis of thionyl chlorides and are dissolved in aether and then ferulic acid methyl ethyl oxalate is obtained after extraction through sodium bicarbonate; under the temperature of between 30 and 50 DEG C, tertiary butyl alcohol is used as reaction medium and the ferulic acid glycerol monobutyralte is obtained through oscillating reaction of the ferulic acid methyl ethyl oxalate, glycerol monobutyralte and Novozym435 lipase in an air bath; the ferulic acid glycerol monobutyralte is dissolved in methanol, filtered and then diluted by HPLC-level methanol; secondly, a step of compartment analysis, which is to adopt a chromatographic column (Ph-3 column Inertsil, IDx250mm) with a column temperature of ambient temperature. A solid phase adopts silica gel filler and the ultraviolet detection wavelength is between 280 and 325 nanometers; a moving phase is gradient mixing of methanol and water; the sample feeding quantity is 5 mu L and the flow speed is 1mL / min. The method selects filler of the chromatographic column and the special ultraviolet scanning wavelength according to the characteristics of materials to be comparted, is quick, high-efficient and accurate and is convenient for industrial analysis and application.

Description

A method for separating and analyzing ferulic acid butyric acid glyceride by high performance liquid chromatography technical field The invention belongs to the field of chromatographic separation, in particular to a method for separating and analyzing ferulic acid butyrin by high performance liquid chromatography (HPLC). Background technique Ferulic acid butyrin is a derivative of natural phenolic acid ferulic acid (Ferulicacid), which is ferulic acid produced by transesterification of ethyl ferulate and tributyrin (TB) The mixture of monobutyrin, ferulic acid dibutyrin and ferulic acid glyceride has many physiological activities of ferulic acid and butyric acid. Therefore, it can be widely used in the food, drug and cosmetic industries. Synthesizing tributyrin with strong fat solubility and ethyl ferulate with strong water solubility can not only achieve mutual protection, reduce the level of oxidative denaturation, and improve their chemical stability, but also The du...

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Problems solved by technology

However, due to the difficulty in controlling the reaction process and the similar polarity of the generated mixture, even the phenomenon of isomers, it is difficult to find suitable separation conditions in the process of analysis and separation, which has brought great troubles to researchers. and impedes research on the properties of these compounds

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