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Extraction separation method of bracken amylose

A separation method and bracken technology are applied in the field of extraction and separation of bracken polysaccharides, which can solve the problems of poor water solubility, low purity and low extraction rate of bracken crude polysaccharides, and achieve good water solubility, high extraction rate, and simple and reasonable method. Effect

Inactive Publication Date: 2008-07-30
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been some reports on the determination of the chemical components of bracken, the characteristics of pharmacological effects, and the value of nutrition and health care, but the extraction methods and especially the separation and purification of bracken polysaccharides have not been reported. Some methods extract bracken polysaccharides containing pigments. Many, gray powder bracken polysaccharides have poor water solubility, low extraction rate and low purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Preparation of Bracken Crude Polysaccharide

[0020] Take 100 g of dried bracken powder, crush it and pass it through a 40-mesh sieve, add 100 ml of 90% ethanol and extract it under reflux at 90° C. for 1 hour. After removing the ethanol, add 2000ml of water, and extract twice on a 65°C water bath, each time for 3 hours. After centrifugation, take the supernatant, add an equal volume of chloroform-n-butanol, the volume ratio of chloroform and n-butanol is 4:1, shake fully, let stand for 10 minutes, and centrifuge at 4500 rpm for 10 minutes. Repeat the above steps until there is no precipitation. The supernatant was concentrated by distillation under reduced pressure at 55°C, and the supernatant was collected, and then precipitated with 2.5 times the volume of ethanol until no precipitate was precipitated. Finally, the precipitate was vacuum freeze-dried at -60°C for 10 hours to obtain crude polysaccharide. Then use the phenol-sulfuric acid method to detect the pol...

Embodiment 2

[0024] 1. Preparation of Bracken Crude Polysaccharide

[0025] Take 100g of dried bracken powder, crush it and pass it through an 80-mesh sieve, add 100ml of 90% ethanol and extract it under reflux at 100°C for 2 hours. After removing the ethanol, add 2500ml of water, and extract 3 times on a 90°C water bath, each time for 4 hours. After centrifugation, take the supernatant, add an equal volume of chloroform-n-butanol, the volume ratio of chloroform and n-butanol is 4:1, shake fully, let stand for 10 minutes, and centrifuge at 4500 rpm for 10 minutes. Repeat the above steps until there is no precipitation. The supernatant was concentrated by distillation under reduced pressure at 50°C, and the supernatant was taken, and then precipitated with 2 times the volume of ethanol until no precipitate was precipitated. Finally, the precipitate was vacuum freeze-dried at -60°C for 12 hours to obtain crude polysaccharide. Then use the phenol-sulfuric acid method to detect the polysacc...

Embodiment 3

[0029] 1. Preparation of Bracken Crude Polysaccharide

[0030] Take 100g of dried bracken powder, crush it and pass it through a 60-mesh sieve, add 100ml of 90% ethanol and extract it under reflux at 80°C for 0.5 hours. After removing the ethanol, add 1500ml of water, and extract twice on a 75°C water bath, each time for 3.5 hours. After centrifugation, take the supernatant, add an equal volume of chloroform-n-butanol, the volume ratio of chloroform and n-butanol is 4:1, shake fully, let stand for 10 minutes, and centrifuge at 4500 rpm for 10 minutes. Repeat the above steps until there is no precipitation. The supernatant was concentrated by distillation under reduced pressure at 60°C, and the supernatant was taken, and then precipitated with 3 times the volume of ethanol until no precipitate was precipitated. Finally, the precipitate was vacuum freeze-dried at -60°C for 8 hours to obtain crude polysaccharide. Then use the phenol-sulfuric acid method to detect the polysacch...

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PUM

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Abstract

The invention discloses an extraction, separation and purification method of a bracken polysaccharide, aiming at solving the problem that the bracken polysaccharide extracted by the prior art method has more pigments, bad water solubility, low yield and low purity. In order to solve the problems, the invention adopts hot water extraction method to leach and ethanol precipitation method to separate and at last purifies the polysaccharide by anion exchange column and gel exclusion chromatography. The invention is suitable for large-scale production and can be widely used for separation and purification of polysaccharide and glycoprotein of all kinds of pteridophyte.

Description

technical field [0001] The invention relates to a method for extracting and separating sugars, more specifically, the invention relates to a method for extracting and separating bracken polysaccharides. Background technique: [0002] Bracken is a general term for edible ferns. Wild bracken has been used as a vegetable and food for famine since ancient times. In the earliest book of poetry in my country, "Poetry Calling for Southern Grass Insects", it is recorded: "It is steeper than Nanshan, and it is said to pick its fern". There are many records in "Qi Min Yao Shu" by Jia Sixie in the Northern Wei Dynasty and "Compendium of Materia Medica" by Li Shizhen in Ming Dynasty. Because wild bracken has a special fragrance taste and is rarely polluted, it is favored by people and has become one of the important export native products in my country. It enjoys the reputation of "the king of mountain delicacies" abroad. Bracken is a traditional wild vegetable, which is extremely nut...

Claims

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Application Information

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IPC IPC(8): A23L1/28A23L1/212A23L1/30A23L33/105
Inventor 罗云波黄昆仑许文涛张方方
Owner CHINA AGRI UNIV
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