Method for testing DNA mononucleotide pleomorphism

Abstract

The invention discloses a detection method of DNA single nucleotide polymorphism. The method which is provided by the invention uses a fluorescent substance for marking the DNA of a sample to be detected, and the nucleotide of the polymorphism site of the target single nucleotide of the DNA to be detected is determined by the fluorescence resonance energy transfer situation of the fluorescent substance and a water-soluble conjugated polymer. The invention has simple and convenient processing, which omits various separation and purification steps; the nucleotide of the polymorphism site of the single nucleotide can be detected by a small amount of DNA, thus the invention has great significance for the development of drugs and the clinical diagnosis of hereditary diseases.

Description

technical field [0001] The invention relates to a method for detecting DNA single nucleotide polymorphism. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) mainly refers to the DNA sequence polymorphism caused by the variation of a single nucleotide at the genome level. [0003] The polymorphism shown by SNP only involves the variation of a single base, which can be caused by the transition or transversion of a single base, or by the insertion or deletion of a base. Theoretically speaking, a SNP may be a two-allelic polymorphism, or a three- or four-allelic polymorphism, but in practice, the latter two are very rare and can almost be ignored. Therefore, the so-called SNPs are biallelic polymorphisms. [0004] Single nucleotide polymorphism is the most common type of heritable variation in humans, accounting for more than 90% of all known polymorphisms. SNPs are widespread in the human genome, with an average of 1 in every ...

AI Technical Summary

Problems solved by technology

[0005] Single nucleotide polymorphism analysis methods often use electrophoresis analysis and solid-phase hybridization methods, such as: DNA sequencing, restriction fragment length polymorphism analysis, single-stranded DNA conformation polymorphism analysis, heteroduplex nucleic acid analysis , allele-specific oligonucleotide hybridization, mismatch analysis, oligonucleotide ligation analysis, etc., require cumbersome separation or washing procedures, increasing the detection time

The above problems can be avoided by using homogeneous detection methods. The current homogeneous detection methods are all based on fluorescence polarization or fluorescence resonance energy transfer between small molecule fluorescent dyes, and the intensity of polarized fluorescence is greatly reduced, resulting in low sensitivity of methods based on fluorescence polarization. High; methods based on fluorescence resonance energy transfer all require double modification of probe molecules, resulting in high cost

Images