Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating single tocopherol from mixed tocopherol

A technology for separating tocopherols, which is applied in the field of separating single tocopherols, and achieves the effects of high degree of production automation, simple process and high production efficiency

Inactive Publication Date: 2008-07-16
ZHEJIANG UNIV
View PDF2 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the separation and purification of tocopherol homologs from SMBC has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating single tocopherol from mixed tocopherol
  • Method for separating single tocopherol from mixed tocopherol
  • Method for separating single tocopherol from mixed tocopherol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Simulated moving bed system CSEP _ 916 (Norr, Germany), equipped with 8 chromatographic columns (ID2×10cm), 2 in each zone, the chromatographic columns are filled with silica gel, and the columns are packed by high-pressure wet method. The mobile phase was n-hexane / ethanol solution (99.5 / 0.5, v / v), and the temperature was 30°C. The purity of the mixed tocopherols used for feeding is 97.5%, which contains 22.8% of α-tocopherol, 2.8% of β-tocopherol, 34.7% of γ-tocopherol and 37.2% of δ-tocopherol. mg / ml feed solution.

[0034] a. Operating conditions

[0035] Injection liquid flow rate: U F =1ml / min

[0036] Eluent flow rate: U D =2.8ml / min

[0037] Raffinate flow rate: U R =2.0ml / min

[0038] Extraction flow rate: U E =1.8ml / min

[0039] Zone 1 velocity: U E =6.4ml / min

[0040] Switching time: t s =757s

[0041] b. Finished product analysis

[0042] The composition of raffinate and extract was analyzed by silica gel column. The purity and yield of δ-toco...

Embodiment 2

[0044] Simulated moving bed system CSEP_ 916 (Norr, Germany), equipped with 8 chromatographic columns (ID2×10cm), 2 in each zone, the chromatographic columns are filled with powdered activated carbon, and packed by high-pressure wet method. The mobile phase was n-hexane / methanol solution (99.5 / 0.5, v / v), and the temperature was 30°C. The purity of the mixed tocopherols used for feeding is 97.5%, which contains 22.8% of α-tocopherol, 2.8% of β-tocopherol, 34.7% of γ-tocopherol and 37.2% of δ-tocopherol. mg / ml feed solution.

[0045] a. Operating conditions

[0046] Injection liquid flow rate: U F =1ml / min

[0047] Eluent flow rate: U D =2.6ml / min

[0048] Raffinate flow rate: U R =1.8ml / min

[0049] Extraction flow rate: U E =2.0ml / min

[0050] Zone 1 velocity: U E =6.7ml / min

[0051] Switching time: t s =720s

[0052] b. Finished product analysis

[0053] The composition of raffinate and extract was analyzed by silica gel column. The purity and yield of δ-tocoph...

Embodiment 3

[0055] Simulated moving bed system CSEP _ 916 (Noel, Germany), equipped with 8 chromatographic columns (ID2×10cm), 2 in each zone, the chromatographic columns are filled with activated alumina, and the columns are packed by high-pressure wet method. The mobile phase was n-hexane / acetone solution (99 / 1, v / v), and the temperature was 30°C. The purity of the mixed tocopherols used for feeding is 97.5%, which contains 22.8% of α-tocopherol, 2.8% of β-tocopherol, 34.7% of γ-tocopherol and 37.2% of δ-tocopherol. mg / ml feed solution.

[0056] a. Operating conditions

[0057] Injection liquid flow rate: U F =1ml / min

[0058] Eluent flow rate: U D =3.1ml / min

[0059] Raffinate flow rate: U R =2.2ml / min

[0060] Extraction flow rate: U E =1.9ml / min

[0061] Zone 1 velocity: U E =6.9ml / min

[0062] Switching time: t s =708s

[0063] b. Finished product analysis

[0064] The composition of raffinate and extract was analyzed by silica gel column. The purity and yield of δ-t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for separating single tocopherol from mixed tocopherol, which takes the mixed tocopherol with a content of 50 percent to 100 percent as a raw material and adopts a four-area simulated moving bed chromatography system to separate a d-alpha-tocopherol, a d-beta-tocopherol, a d-gamma-tocopherol and a d-delta tocopherol with the purity and yield all more than 98 percent. As the simulated moving bed chromatography separation is a continuous process, the method has the advantages of high degree of production automation, simple technique, high production efficiency, continuous production and stable product quality. As an adsorbent and a solvent have low consumption, the whole separating device does not relate to chemical reactions.

Description

technical field [0001] The invention relates to chemical separation technology, in particular to a method for separating individual tocopherols from mixed tocopherols. Background technique [0002] Natural vitamin E (scientific name tocopherol) is a mixture mainly composed of four tocopherol homologues derived from tocol, including: d-α-, d-β-, d-γ- and d-delta-tocopherol, they all have saturated C16 side chains, and differ only in the number and position of methyl groups on the benzene ring. Although their structures are very similar, their biological activities are different, among which: d-α-tocopherol (activity is 100)>d-β-tocopherol (activity is 10-50)>d-γ-tocopherol (activity 10)>d-δ-tocopherol (activity 1). [0003] Natural vitamin E is widely used in medicine, cosmetics and food industry as an antioxidant and nutritional supplement. It is superior to synthetic vitamin E in terms of biological activity, nutritional physiological effects and use safety. How...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/72
Inventor 杨亦文吕裕斌任其龙吴平东苏宝根吴彩娟苏云黄梅
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com