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Method for preparing microorganism immobilization embedded particles

A technology for immobilizing microorganisms and granules, applied in biochemical equipment and methods, immobilized enzymes, immobilized on or in inorganic carriers, etc., can solve the problem of weak resistance to temperature changes and pH changes, and poor particle diffusion and mass transfer performance , Unfavorable fixation of strains of other genera, etc., to achieve the effects of enhanced competitiveness, good heat preservation performance, and improved diffusion and mass transfer performance

Inactive Publication Date: 2008-05-14
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the embedding immobilization method has been widely used in the remediation of contaminated fluid media, it has not been successfully applied to the remediation of contaminated non-fluid media.
The main reason: on the one hand, because of the complexity and particularity of the non-fluid medium itself, this technology poses many scientific problems that are different from those faced when applying this technology in fluid media, such as how to choose the carrier of immobilized microbial particles , how to overcome the non-recoverability of immobilized particles in non-fluid media, how to overcome the impact of the heterogeneity of pollutant concentration on immobilized particles; how to improve the mechanical strength of immobilized particles to resist physical damage caused by environmental stress On the other hand, there are defects in the embedding method itself, such as: poor diffusion and mass transfer properties of particles, uneven voids, weak resistance to temperature changes and pH changes, etc.
In addition, the traditional Na Alg immobilization method often corresponds to a single bacterial species, lacks broad-spectrum adaptability, and is unfavorable for the immobilization of other genera strains.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 (comparative example)

[0030] (1) Bacterial slant culture

[0031] Insert Bacillus sp. into test tubes of beef extract protein base medium (0.3% beef extract, 1.0% protein base, 0.5% NaCl, 1.5-2.0% agar, pH 7.0-7.2) and place them at 28 Cultivate in ~30°C incubator for 48h.

[0032] (2) Preparation of bacterial seed solution

[0033] Receive 2 loops of bacterial lawns on the slant medium with an inoculation loop respectively, and insert into the liquid seed medium (glucose 1.25%, beef extract 0.25%, NH 4 NO 3 0.1%, MgSO 4 7H 2 O 0.02%, KCl 0.02%, pH 7.0-7.2), under the conditions of shaking table rotation speed 130rpm-150rpm, 28-30°C, cultivate for 16-20 hours to obtain bacterial seed liquid.

[0034] (3) Preparation of gel solution

[0035] According to the total mass of 200g, weigh 2% sodium alginate (Na·Alg) and 1% gelatin, soak in 40g tap water for 12-20 hours, after constant volume, sterilize with damp heat at 110°C for 30min, cool to 35-45°C...

Embodiment 2

[0047] Embodiment 2 (comparative example)

[0048] One of the differences from Example 1 is: when preparing the cross-linking agent, according to the total 600g, mix 4% CaCl 2 ~ Saturated boric acid aqueous solution with natural pH.

[0049] The second difference is that: when preparing the reinforcing agent, according to the total mass of 600g, mix 1.0% chitosan aqueous solution, first dissolve it with a small amount of concentrated HCl, then add water to make it constant, and the pH is natural.

[0050] The mechanical strength of the prepared immobilized particles is only 1.00kg / cm 2 , when granulating, the granules are thin, the granulation is uneven, and the granules contain a lot of foam. The prepared immobilized granules were multiplied and cultured on a shaking table, and used for the remediation of PAHs pollution in the soil. When the initial PYR / BaP concentration in the soil was 50mg / kg, the pellet ratio was 12%, and the removal rate of PYR was 42 days after remedia...

Embodiment 3

[0052] One of the differences from Example 1 is that when the gel solution is prepared, 3% sodium alginate (Na Alg) and 0.3% of activated carbon (150 mesh) are weighed according to the total mass of 200 g, and soaked in 40 g of tap water for 12 hours. After storage, sterilize with moist heat at 110°C for 30 minutes, and cool to 35°C.

[0053] The second difference from Example 1 is that when preparing the reinforcing agent, a 4% PVA aqueous solution is mixed with a total mass of 600 g, and the pH is natural.

[0054] The third difference from Example 1 is: during the preparation of immobilized granules, the seed liquid is mixed with the gel liquid, stirred rapidly for 5 minutes, packed into a glass granulator with an outlet diameter of 1.00 mm, and the outlet droplet velocity is controlled by vacuum suction. 20 drops / min

[0055] The mechanical strength of the immobilized particles prepared according to the above steps reaches 22.12kg / cm 2 ,. After multiplication and cultiv...

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Abstract

The invention relates to microorganism immobilization technology, in particular to a method for preparing immobilized granules for remediation of polycyclic aromatic hydrocarbons (PAHs) polluted soil. Mix the glue evenly, and pass through a granulator with a diameter of 1-4.00mm under natural conditions of 25-40°C and pH to prepare immobilized granules at a dripping speed of 20-30 drops / min. Chemically cross-link, then stand in the fixative for 23-58 hours, and finally soak in sterile water for 24-72 hours, rinse off the residual liquid, multiply and cultivate, and set aside; the mass ratio of the gel solution is: alginic acid Sodium (Na·Alg) 2-3%, (150 mesh) activated carbon, red brick powder, peat soil and / or fly ash 0.3-0.6%, and the balance is water. The invention can greatly improve the mechanical strength, elasticity and slow-release performance of the immobilized particles.

Description

technical field [0001] The invention relates to microorganism immobilization technology, in particular to a method for preparing immobilized particles for polycyclic aromatic hydrocarbons (PAHs) polluted soil remediation, which uses sodium alginate (Na·Alg) as the main carrier material and peat The technology of embedding and immobilizing microorganisms with soil, red brick powder, fly ash, etc. as auxiliary carriers can greatly improve the mechanical strength, elasticity and slow-release performance of the immobilized particles. Background technique [0002] There are some defects when the traditional free microbial technology is used to remediate PAHs-contaminated soil, such as low concentration of effective degrading bacteria per unit volume, slow reaction initiation, inferior competition with indigenous bacteria, poor resistance to toxicity, and sensitivity to environmental conditions. Microorganisms in the remediation site environment may have large discrepancies betwee...

Claims

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Application Information

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IPC IPC(8): C12N11/00C12N11/04C12N11/14B09C1/10
Inventor 苏丹李培军鞠京丽
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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