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Method for preparing bone grafting material with pilose antler and deer bone as material and the bone grafting material prepared by the method

A technology of velvet antler and deer bone, which is applied in the field of preparation of bone graft materials, can solve problems such as adverse effects of clinical application, cross-infection, and fear of patients, and achieve the effect of good bone conduction, reasonable price, and abundant resources

Inactive Publication Date: 2008-03-26
王喜文
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Bone transplantation is an important means of orthopedic repair surgery. Research on bone banks and bone graft materials has always been an important topic in orthopedics. Bone banks at home and abroad mainly store allogeneic bone, but due to the limited source of allogeneic bone, there are potential risks of hepatitis B and AIDS. The risk of cross-infection cannot meet the various needs of clinical bone graft materials. In recent years, there have been recombined heterogeneous bone materials in China. This product is a composite of BMP extracted from bovine cortical bone and bovine cancellous bone carrier without antigen Through a series of animal experiments and clinical studies, it has been proved that it not only induces osteogenesis and good bone conduction, but also has good biocompatibility. It is an applicable bone graft material. Due to the mad cow disease in Europe, the The clinical application of domestic recombined heterogeneous bone products has brought adverse effects, and some patients have a psychological fear of bone graft materials extracted from bovine bones that can promote osteogenesis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Take the Ergang of velvet antler as a raw material, weigh 100 grams, and grind it into a fine powder of 100 mesh with a pulverizer. Take out half and add 200ml of 0.1Mole / L sodium carbonate buffer solution, the pH is 8.5-8.8, stir at room temperature for 3 hours, add acetone 100ml and continue stirring for 2 hours, filter to obtain the extract for use. Add the other half of deer antler powder to 200ml of 0.001 / L hydrochloric acid solution, the pH is 3.5-4.0, stir at room temperature for 3 hours, add 100ml of acetone and continue to stir for 2 hours, filter the extract and mix it with the obtained extract, and then extract the mixture Place the solution in a freezer for refrigeration, add 2500ml of cold acetone, and dehydrate for two times to obtain a precipitate. Dissolve the precipitate with 250ml of pyrogen-free water, and ultrafilter it through an ultrafilter with a cut-off of 70,000 Daltons, and collect molecular weight less than 70,000 Daltons The part with a cut-o...

Embodiment 2

[0045] Take the lotus flower of red deer antler as raw material, weigh 100 grams, and grind it into 100-mesh fine powder with a pulverizer. Weigh 100 grams and grind it into 100 mesh fine powder with a pulverizer. Take out half and add 200ml of 0.1Mole / L sodium carbonate buffer solution to adjust the pH to 8.5. After stirring at room temperature for 3 hours, add 100ml of acetone and continue stirring for 2 hours, and filter to obtain the extract for use. Add the other half of deer antler powder to 200ml of 0.001Mole / L hydrochloric acid solution, adjust the pH to 4, stir at room temperature for 3 hours, add 100ml of acetone and continue stirring for 2 hours, filter the extract and mix it with the obtained extract, and then extract the mixture Place the solution in a freezer for refrigeration, add 2500ml of cold acetone, dehydrate twice to obtain a precipitate, dissolve the precipitate with 250ml of sterile water, and ultrafilter through an ultrafilter with a cut-off of 70,000 D...

Embodiment 3

[0049] Take the Sancha of velvet antler as a raw material, weigh 100 grams, and grind it into a fine powder of 100 mesh with a pulverizer. Take out half and add 200ml of 0.1Mole / L sodium carbonate buffer solution, the pH is 8.5-8.8, stir at room temperature for 3 hours, add acetone 100ml and continue stirring for 2 hours, filter to obtain the extract for use. Add the other half of deer antler powder to 200ml of 0.001Mole / L hydrochloric acid solution, adjust the pH to 3.5-4, stir at room temperature for 3 hours, add 100ml of acetone and continue to stir for 2 hours, filter the extract and mix it with the obtained extract, then Put the mixed extract in a freezer, add 2500ml of cold acetone, and dehydrate twice to obtain a precipitate. Dissolve the precipitate in 250ml of sterile water, ultrafilter through an ultrafilter with a cut-off of 70,000 Daltons, and collect molecular weight less than 70,000 The part with a molecular weight of less than 50,000 Daltons is filtered through ...

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Abstract

The present invention discloses a method for preparing bone grafting material by using velvet deerhorn and deer's bone as raw material and bone grafting material prepared by adopting said preparation method. It is characterized by that said invention utilizes the extract of velvet deerhorn and deer's cancellous bone to prepare bone-grafting material, its resource is rich and its application is safe, and said bone-grafting material contains several growth factors and velvet deerhorn polypeptide, so that it has obvious bone induction action, and can obtain extensive application.

Description

technical field [0001] The invention relates to a method for preparing a bone graft material, in particular to a method for preparing a bone graft material with deer antler and deer bone as raw materials and the bone graft material prepared by the method. Background technique [0002] There are many farms in Jilin, Liaoning, Heilongjiang, Hebei, Beijing and other places in China to raise sika deer and red deer. The medical and pharmaceutical value of sika deer and red deer has attracted more and more attention. The first is deer antler, which is the young horns of sika deer. More than 1 to 2 branches. Those with 1 branch are commonly called "Ergang", and those with 2 branches are commonly called "Sancha". Red deer antler is also known as green hairy. It is the young antler of red deer, which is thicker than flower antler and has many branches. One side branch is called "Single Gate", two are called "Lotus", three are called "Sancha", and four are called "Sicha". ",Or mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 李德山王喜文
Owner 王喜文
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