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Method for preparing astragaloside iv by enzymic hydrolysis for astragalus saponin glycosyl

A technology of astragaloside IV and astragaloside, which is applied in the field of enzymatically hydrolyzing astragaloside glycosides to prepare astragaloside IV, can solve the problems of low content of astragaloside IV, overcome the destructiveness of saponins, improve the extraction rate, and have strong purpose Effect

Inactive Publication Date: 2008-02-27
金凤燮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The present invention aims to solve the technical problem of low content of astragaloside in the prior art, and provides a kind of saponin glycosyl (containing three and four glycosyls) with high content in astragalus by enzymatic hydrolysis to prepare astragaloside Ⅳ method, no pollution, strong purpose, high conversion rate

Method used

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  • Method for preparing astragaloside iv by enzymic hydrolysis for astragalus saponin glycosyl
  • Method for preparing astragaloside iv by enzymic hydrolysis for astragalus saponin glycosyl
  • Method for preparing astragaloside iv by enzymic hydrolysis for astragalus saponin glycosyl

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Embodiment 1

[0015] a. Aspergillus niger (Aspegillus niger) was cultured with agitation and ventilation at a temperature of 28-30°C for 50-100 hours in a medium containing 5% malt water extract and 1% enzyme-producing inducer-Astragalus water extract , centrifuged and sterilized to get the supernatant (containing enzyme mixture), in the supernatant, use 60-75% saturated ammonium sulfate to precipitate the enzyme protein, collect the protein, and dissolve it in acetic acid buffer commonly used in 1 / 10 of the fermentation broth volume. Solution (0.02M, pH 5.0), or phosphate buffer, Tris buffer, dialyzed to remove ammonium sulfate, centrifuged to remove slag, which is the enzyme solution;

[0016] b. Mix 50 ml of the above enzyme solution, 3 g of astragaloside and 100 ml of acetic acid buffer (0.02M, pH 5.0), react at a temperature of 20-55°C for 6-24 hours, and then add 1 / 3 volume of The saponins were extracted three times with n-butanol, and evaporated to dryness under reduced pressure to o...

Embodiment 2

[0019] a. Aspergillus oryzae in water containing 5% wheat bran water extract, 0.02% enzyme-producing inducer-soybean isoflavone or rutin containing flavonoids, or 0.6% of its flavonoid source plant-Sophora japonica In the culture medium of the extract, under the condition of a temperature of 28-30°C, agitate and ventilate for 50-100 hours, centrifuge and sterilize to obtain a supernatant (containing an enzyme mixture), and use 60-75% saturation of the supernatant in the supernatant Ammonium sulfate precipitates enzyme protein, collects protein, dissolves in commonly used acetate buffer (0.02M, pH 5.0), or phosphate buffer, Tris buffer of 1 / 10 of the fermentation broth volume, dialyzes to remove ammonium sulfate, centrifuges to remove slag , which is the enzyme solution;

[0020] b. Treat astragaloside with enzyme liquid according to the method of Example 1, purify and detect the result of the enzyme reaction product: the product is mainly astragaloside IV, and the conversion r...

Embodiment 3

[0023] Use the method of Example 1 or Example 2 to prepare 40 ml of enzyme liquid, add it to 10 grams of Astragalus slices or powder, soak the Astragalus slices at a temperature of 35-40°C for 4-40 hours, dry the Astragalus slices, and use high-efficiency liquid Phase chromatography (document 7) measures the content of astragaloside IV in Huangqi tablets, which is more than 6 times higher than that in the original Huangqi tablets or powder.

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Abstract

The present invention discloses a method for preparing astragalus methylglucoside by utilizing enzymatic hydrolysis of astragalus saponin glycosyl. It is characterized by that it uses an enzyme method to hydrolyze astragalus saponin glycosyl to prepare astragalus methylglucoside. The described enzyme is prepared by using astragalus saponin or flavone or isoflavone as fermentation enzyme-producing inductor and utilizing bacteria, streptomycete, mold, saccharomycetes and basidiomycetes to make fermentation. Said invention can be used for preparing astragalus tablet and powder products with high astragalus methylglucoside content.

Description

Technical field: [0001] The present invention relates to a method for preparing low-glycoside astragaloside, in particular to a method for preparing astragaloside IV by enzymatically hydrolyzing astragaloside sugar base with an enzyme capable of hydrolyzing astragaloside sugar base to increase the content of astragaloside IV Methods. Background technique: [0002] Astragalus is one of the commonly used traditional Chinese medicines, which has the effects of improving human immunity, invigorating qi, lowering blood pressure, strengthening the heart, reducing inflammation and swelling, and antiviral. At present, the main varieties used for medicine in my country are Astragalus membranaceus [Fisch.] Bge var. mongholicus and Astragalus membranaceus [Fisch.] Bge [Phaca membranacea Fisch.]. The main active ingredients in Astragalus membranaceus are astragalus saponins (fresh The saponin glycosyl in Astragalus root often has acetyl groups, but the acetyl groups are removed during t...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12R1/66
Inventor 鱼红闪金凤燮
Owner 金凤燮
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