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Cordyceps sinensis asexual propagation method

A technology of Chinese hairy sporophyte and asexual reproduction, applied in the direction of fungi, etc., can solve problems such as production cost constraints and technical difficulties, and achieve the effect of stable traits

Inactive Publication Date: 2008-02-20
姜善清
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Completely imitating the law of sexual reproduction of Cordyceps sinensis in nature to cultivate Trichosporum, currently there are still considerable technical difficulties, and is restricted by production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0034] According to the preparation of 100kg medium,

[0035] (1), soak 4 kg of shelled and peeled scallop meat with clear water for 48 hours, then change the water for more than 6 times to desalinate, finally grind it into a slurry, and use it as component A;

[0036] (2), 0.5 kg of peanuts were soaked in water for 10 hours, and then refined into component B;

[0037] (3), 2kg of fresh beef is refined into C component;

[0038] (4), 8 kg of silkworm chrysalis are dehumidified and peeled to make D component;

[0039] (5) Mix A, B, C, and D components, add water to make up to 80kg, boil, simmer for 30 minutes, precipitate, filter out slag, and make E component;

[0040] (6), boil 5 kg of fresh beef bones with a pressure cooker and boil for 30 minutes, precipitate and filter out the slag, and make the F component;

[0041] (7) Mix components E and F evenly, add 5kg of fresh milk, 1kg of soybean peptone, 1.2kg of agar, 50g of potassium dihydrogen phosphate, and 20g of magnesium ...

example 2

[0043] According to the preparation of 100kg medium,

[0044] (1) Soak 3 kg of shelled and peeled scallop meat in clear water for 36 hours, then change the water for more than 6 times to desalinate, and finally grind it into a slurry to make A component;

[0045] (2), 0.6 kg of peanuts were soaked in water for 8 hours, and then refined into component B;

[0046] (3), 1.5kg of fresh beef is refined into C component;

[0047] (4), 9 kg of silkworm chrysalis are dehumidified and peeled to make D component;

[0048] (5), mix A, B, C, and D components, add water to make up to 85kg, boil, simmer for 20 minutes, precipitate, filter out the slag, and make E component;

[0049] (6), boil 4 kg of fresh beef bones with a pressure cooker and boil for 20 minutes, precipitate and filter out the slag, and make the F component;

[0050] (7) Mix components E and F evenly, add 6kg of fresh milk, 0.8kg of soybean peptone, 1.4kg of agar, 40g of potassium dihydrogen phosphate, 15g of magnesium ...

example 3

[0052] According to the preparation of 100kg medium,

[0053] (1), soak 5 kg of shelled and peeled scallop meat with clear water for 60 hours, then change the water for more than 6 times to desalinate, and finally grind it into a slurry to make A component;

[0054] (2), 0.4 kg of peanuts were soaked in water for 12 hours, and then refined into component B;

[0055] (3), 2.5kg of fresh beef is refined into C component;

[0056] (4), 7kg of silkworm chrysalis is refined and peeled to make D component;

[0057] (5) Mix A, B, C, and D components, add water to make up to 75kg, boil, simmer for 40 minutes, precipitate, filter out residue, and make E component;

[0058] (6), boil 6 kg of fresh beef bones with a pressure cooker and boil for 40 minutes, precipitate and filter out the slag, and make the F component;

[0059] (7) Mix components E and F evenly, add 4kg of fresh milk, 1.2kg of soybean peptone, 1.0kg of agar, 60g of potassium dihydrogen phosphate, 25g of magnesium sulfa...

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Abstract

The invention relates to a cloning method of a Chinese hirsutella, which is characterized in that: a bacteria tissue is cut into small pieces by operation knife in a steam deinfected glass bottle, inoculated on the surface of a medium in the glass bottle; the glass bottle is covered with an air permeable cover, and placed in a culativation region until the bacteria tissue is ripe. The invention makes use of the monosporous splitting breeding principle of fungus hirsutella, and successfully prepares qualified bacteria tissue with sexual characteristics of hirsutella that can be a substitute of natural Cordyceps aweto by cloning method.

Description

technical field [0001] The invention relates to a method for asexual reproduction, in particular to a method for asexual reproduction of Mortierella chinensis. The synonym of Hirsutella Sinensis Liu, Guo, Yu et Zeng is the species of the genus Cephalosporum of the fungus Cordyceps sinensis. Background technique [0002] The growth process of Cordyceps sinensis that grows in nature is actually that the larvae of the bat moth are infected by the trichospores, which are fungi, during the feeding process. The head of the fungus forms a collection of bacteria, which is called a spore, and the top produces spores, which burst out after maturity, reinfect, and go round and round to complete the entire process of sexual reproduction by the hairy sporophyte. Strictly speaking, Cordyceps sinensis is neither a worm nor a grass, but a combination of insect larvae and fungus hairy spores. [0003] Completely imitating the law of sexual reproduction of Cordyceps sinensis in nature to cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 姜善清
Owner 姜善清
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