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Peanut protein active polypeptide and method for making same

A technology for active polypeptide and peanut protein, which is applied in the field of biologically active polypeptide and its preparation, can solve the problems of low peptide yield, high production cost, side reactions, etc., and achieves the effects of good solubility, stability and good solubility

Inactive Publication Date: 2008-01-30
张吉秀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous development of science and technology and the maturity of peanut protein development and utilization technology, peanut protein deep-processing products with good functional properties and physiological activities are booming. Traditional methods for preparing bioactive peptides include natural extraction, chemical synthesis, acid-base method and genetic engineering method, but the disadvantages of these methods are: low peptide yield, high production cost and side effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: The preparation process of peanut protein active polypeptide:

[0020] Peanut → low temperature oil extraction → crude oil refining → refined oil

[0021] ↓

[0022] Peanut soybean meal→ultrafine grinding→semi-skimmed protein powder→enzymolysis→separation→fermentation enzymolysis→separation→ultrafiltration→desalination→concentration→drying→peanut protein active polypeptide (finished product). The specific preparation process is:

[0023] 1. Raw material pretreatment: Peanut cake is super crushed, diluted with water, passed through a 150-350 mesh sieve to make powdered peanut protein, adjusted the pH value to slightly alkaline with alkali and adjusted the solution temperature to the optimum temperature for enzyme reaction, and then added compound Enzyme A is reacted, and then the peanut protein powder is further purified:

[0024] (1) Peanut protein powder→water soluble (1:15 is the weight ratio of powder to water)→alkaline extraction (use ...

Embodiment 2

[0030] Embodiment 2: Functional experiment of peanut protein active polypeptide

[0031] (1) Determination of hypertensive activity

[0032] Add 100ul sample to 100ul 0.05M borate buffer solution, the pH of the buffer solution is 8.5, including 0.3M Nacl, 25mU angiotensin-converting enzyme (ACE), preheat at 37°C for 9 minutes; add 150ul hippuryl histidyl Start the reaction with leucic acid (HHL), react at 37°C for 0.8h, add 250ul of 1M hydrochloric acid to terminate the reaction; then add 1.5ml of ethyl acetate to extract hippuric acid, vortex and mix and then centrifuge (2000g, 15min); absorb 1ml of ethyl acetate layer into another test tube, dried, redissolved in 3ml deionized water after cooling, and measured its absorbance value at 228nm.

[0033] ACE inhibitory activity (%)=(A a -A b ) / (A a -A c )*100%

[0034] where A a : optical density in the absence of inhibitor

[0035] A b : optical density in the presence of inhibitor and enzyme

[0036] A c : optical de...

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Abstract

The invention discloses a continuous zymolysis synthesis technique of peanut protein active peptides and the preparation method thereof. The active peptides account for more than or equal to 90 percent of the total proteins, and the protein active peptides account for more than or equal to 80 percent of the total proteins, fat is less than equal to 1 percent, water is less than equal to 6 percent, ash is less than equal to 2 percent. The invention uses peanut protein as raw materials, and prepares peanut protein active peptide through continuous zymolysis synthesis technique, compared with powder protein, the peanut protein active peptides have better dissolubility, fluidity and stability, therefore, the peanut protein active peptides can be widely applied to such fields as medicine, everyday chemical and foods, etc.

Description

Technical field: [0001] The invention relates to a bioactive polypeptide and a preparation method thereof, in particular to a continuous fermentation enzymolysis synthesis process and a preparation method of a peanut protein active polypeptide. technical background: [0002] Peanut is one of the main oil crops in my country, and most of the peanut cake after oil extraction is used for processing feed. With the continuous development of science and technology and the maturity of peanut protein development and utilization technology, peanut protein deep-processing products with good functional properties and physiological activities are booming. Traditional methods for preparing bioactive peptides include natural extraction, chemical synthesis, acid-base method and genetic engineering method, but the disadvantages of these methods are: low peptide yield, high production cost and side effects. Invention content: [0003] In order to overcome the deficiencies in the prior art...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/14
Inventor 张吉秀
Owner 张吉秀
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