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Fluorescence rubber latex quantitative chromatography indicator paper and manufacture method thereof

A fluorescent glue and test strip technology, applied in the field of medical testing, can solve the problems of single, unable to change color, unable to realize quantitative detection, etc., and achieve the effect of wide application range and variable color.

Active Publication Date: 2007-08-15
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (3) The colloidal gold labeling process is a physical adsorption process, so the stability in the liquid phase is poor
[0006] (4) After color development, it can only be a single color - purple, and the color cannot be changed according to different varieties or different requirements
[0007] (5) Accurate quantitative detection cannot be achieved

Method used

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  • Fluorescence rubber latex quantitative chromatography indicator paper and manufacture method thereof
  • Fluorescence rubber latex quantitative chromatography indicator paper and manufacture method thereof
  • Fluorescence rubber latex quantitative chromatography indicator paper and manufacture method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1: Fluorescent latex quantitative chromatography test strip (double antibody sandwich) for diagnosis of hepatitis B surface antigen

[0035] As shown in Figure 1a, fluorescent latex quantitative chromatography test paper strip 11, this test paper strip is to stick sample pad 112, glass fiber membrane 113, coating film 114 and water-absorbing paper 115 on the backing 111 successively, wrapping A detection zone 114a and a control zone 114b are arranged on the film, the detection zone 114a is coated with hepatitis B surface antigen monoclonal antibody, the control zone 114b is coated with anti-mouse antibody, and the distance between the detection zone 114a and the control zone 114b is about 5mm. The glass fiber membrane 113 is coated with fluorescent latex particles labeled hepatitis B surface antigen monoclonal antibody protein, as shown in FIG. 1 and FIG. 2 .

[0036] The fluorescent latex quantitative chromatography test strip for diagnosing HBsAg is prepare...

Embodiment 2

[0050] Embodiment 2: Fluorescent latex quantitative chromatography test strip (double antigen sandwich) for diagnosing AIDS HIV1 / 2 type antibody

[0051] Fluorescent latex quantitative chromatography test strip 11, the test strip is a bottom substrate 111, which is successively pasted with a sample pad 112, a glass fiber membrane 113, a coating film 114 and a water-absorbing paper 115, and the coating film is provided with a detection area 114a and the control area 114b, the detection area 114a is coated with HIV gp36, gp41 and gp120 genetically engineered recombinant antigens, the control area 114b is coated with anti-mouse antibody, and the distance between the detection area 114a and the accusation area 114b is about 5mm. The glass fiber membrane 113 is coated with fluorescent latex particles labeled H1Vgp36, gp41 and gp120 genetically engineered recombinant antigens.

[0052] The fluorescent latex quantitative chromatography test strip for diagnosing HIV1 / 2 antibodies is p...

Embodiment 3

[0060] Embodiment three: Realize AIDS, hepatitis B surface antigen (one inspection multiple items)

[0061] Fluorescent latex quantitative chromatography test strip 11, the test strip is a bottom substrate 111, which is successively pasted with a sample pad 112, a glass fiber membrane 113, a coating film 114 and a water-absorbing paper 115, and the coating film is provided with a detection area 114a (detection area 114a has two detection lines) and control area 114b (control line), one detection line is coated with HIV gp36, gp41 or gp120 genetically engineered recombinant antigen, and the other detection line is coated with hepatitis B surface antigen monoclonal antibody, The control area 114b is coated with an anti-mouse antibody, and the distance between the two detection lines and the control line is about 5mm. The glass fiber membrane 113 is coated with HIV gp36, gp41 or gp120 genetically engineered recombinant antigens marked with fluorescent latex particles and monoclon...

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Abstract

This invention discloses one fluorescent emulsion quantitative diagnostic apparatus bar and its process method, wherein, the test bar comprises orderly on the underlay earth connection sample pad, glass fiber film, cover film and water absorptive water, wherein, the cover film is set with cover antibody or antigen test area and anti-mouse control area; the said glass fiber film is coated with label protein. the process method comprises the following steps: emulsion covalence activating; fluorescence emulsion micro particle label protein processing; antigen or antibody covering onto aqua fortis fiber film and its enclosing; on underlay orderly connecting sample pad, glass fiber film of the protein, cover film and water absorptive paper to get the test board.

Description

technical field [0001] The invention belongs to the field of medical examination, and relates to a fluorescent latex quantitative chromatography diagnostic test strip and a preparation method thereof. Background technique [0002] In immunoassay, methods such as radioimmunoassay, enzyme-linked immunosorbent assay (ELISA) and colloidal gold labeling are mainly used at home and abroad to achieve quantitative or qualitative detection. These methods all have defects in varying degrees. Taking the immunocolloidal gold chromatography test strip widely used at present as an example, there are following deficiencies: [0003] (1) Colloidal gold is a kind of nanoparticle. It is difficult to prepare colloidal gold nanoparticles, and it is difficult to control the particle diameter uniformly. [0004] (2) A strong reducing agent is used in the colloidal gold labeling process, and at the same time, it is exposed to heavy metal ions, which has a certain impact on human health and safet...

Claims

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Application Information

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IPC IPC(8): G01N33/544G01N33/558G01N33/577G01N21/64
Inventor 王继华
Owner GUANGZHOU WONDFO BIOTECH
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