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Acid and conjugated enzyme process for preparing fucan in lower molecular weight

A technology of fucoidan and low molecular weight, applied in the field of preparation of low molecular weight fucoidan by acid method combined with enzymatic method, can solve the problems of severe acid hydrolysis reaction conditions, difficult operation, expensive price, etc., and achieve sulfate group shedding Low efficiency, low cost, simple operation

Active Publication Date: 2009-12-30
厦门市燕之屋丝浓食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the strong acid hydrolysis reaction conditions are severe, it is difficult to operate, and the product needs to be desalted; while the specific seaweed polysaccharide sulfate hydrolase is expensive and the output is small

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for preparing low-molecular-weight fucoidan by an acid method combined with an enzyme method, comprising the following steps:

[0014] (1) Take 1000kg of dried kelp and add acid in a ratio of 1:9. After extracting for 2 hours, centrifuge to collect the supernatant and add ethanol solution to 30% saturation. Collect the supernatant and then add ethanol solution to 70% saturation and stir Evenly, let it stand for 20 minutes to precipitate, and centrifuge the solution to collect the precipitated fucoidan, which is freeze-dried.

[0015] (2) The extracted fucoidan is ultrafiltered with a membrane with a molecular weight cut-off of 1000 Da, and protein is removed by protease at 37° C. and pH=2-3. 21 g of crude fucoidan product was obtained. Take 1 g of crude fucoidan, and use protease to remove protein at 37°C and pH=2-3.

[0016] (3) Add pectinase, heat and hydrolyze at 30°C for 2 hours; adjust the pH value to 4 with acid, let stand for 2 hours, filter or centrif...

Embodiment 2

[0019] A method for preparing low-molecular-weight fucoidan by an acid method combined with an enzyme method, comprising the following steps:

[0020] (1) Take 1000kg of dried kelp and add acid at a ratio of 1:10. After extracting for 4 hours, centrifuge to collect the supernatant and add ethanol solution to 40% saturation. Collect the supernatant and then add ethanol solution to 80% saturation and stir Evenly, let it stand for 20 minutes to precipitate, and centrifuge the solution to collect the precipitated fucoidan, which is freeze-dried.

[0021] (2) The extracted fucoidan was ultrafiltered with a membrane with a molecular weight cut-off of 5000 Da, and protein was removed by protease at 45° C. and pH=2 to obtain 21 g of crude fucoidan product. Take 1 g of crude fucoidan, and use protease to remove protein at 45° C. and pH=2.

[0022] (3) Add pectinase, heat and hydrolyze at 45°C for 3 hours; adjust the pH value to 5 with acid, let stand for 3 hours, filter or centrifuge ...

Embodiment 3

[0025] (1) Take 1000kg of dried kelp and add acid in a ratio of 1:8. After extracting for 3 hours, centrifuge to collect the supernatant and add ethanol solution to 35% saturation. Collect the supernatant and then add ethanol solution to 75% saturation and stir Evenly, let it stand for 20 minutes to precipitate, and centrifuge the solution to collect the precipitated fucoidan, which is freeze-dried.

[0026] (2) The extracted fucoidan is ultrafiltered with a membrane with a molecular weight cut-off of 1000 Da, and the protein is removed by enzyme at 40° C. and pH=2.5 to obtain a crude fucoidan product. Take the crude fucoidan, and use protease to remove protein at 40°C and pH=2.5.

[0027] (3) Add pectinase, heat and hydrolyze at 35°C for 2.5 hours; adjust the pH value to 4.5 with acid, let stand for 2.5 hours, filter or centrifuge to remove impurities, and take the supernatant.

[0028] (4) The polysaccharide aqueous solution concentrated by membrane ultrafiltration with a m...

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PUM

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Abstract

The present invention discloses acid and conjugated enzyme process for preparing fucan in lower molecular weight. Coarse fucan is enzymolyzed with commercial pectinase under controlled enzymolysis time, temperature and pH value and intercepted with ultrafiltering film of molecular weight 1000-5000 Da to obtain low molecular weight fucan. The present invention has simple operation, mild condition, high yield, low sulfate radical dropping rate, low cost and other advantages, and is easy use in industrial production. The present invention has simultaneous fucan extraction and lower molecular weight fucan preparation, and has simplified technological process, high production efficiency and over 30 % lowered production cost. In addition, the process uses no harmful chemical reagent, and the product is safe, non-toxic and especially suitable for producing functional food and medicine.

Description

technical field [0001] The invention relates to a method for extracting fucoidan, in particular to a method for preparing low molecular weight fucoidan by using an acid method combined with an enzyme method. Background technique [0002] Fucoidan is a natural substance with high biological activity in seaweed. It has anti-tumor, anti-virus, anti-coagulation, lowering blood sugar and blood lipids, regulating immunity, scavenging active oxygen free radicals, and anti-oxidation in vivo and in vitro. effect. Fucoidan is a water-soluble polysaccharide containing a variety of monosaccharides and sulfate groups, with molecular masses ranging from tens of thousands to hundreds of thousands. Its special structure and function have attracted the attention of many experts and scholars. With the deepening of research on the antiviral mechanism of polysaccharides, it is found that there are many factors that affect the activity of polysaccharides, such as the high-level structure of po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04
Inventor 吴永沛刘明
Owner 厦门市燕之屋丝浓食品有限公司
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