Preparation process of collagen-based surface wound repairing membrane possessing tissue induction

Abstract

The invention discloses a preparation method of a tissue-inducible collagen-based wound repair membrane, which is characterized in that the collagen-based repair membrane is physically and chemically modified, and gelatin-based slow-release microspheres containing cell growth factors are introduced. The repair membrane material has a pore size of 20-200 microns, a porosity of ≥80%, a tensile strength of ≥1MPa, and a pH value of 5.0-6.0. It has good degradability and biocompatibility. The pore structure and active collagen components of the membrane determine that it can fully absorb wound exudate, and can induce autologous cell adhesion, proliferation, differentiation, and ingrowth of blood vessels, and the compound slow-release microspheres can make cell growth factors last for a long time. The effective release to the wound can significantly shorten the wound healing time, and the healed new tissue is well integrated with the surrounding tissue, avoiding the generation of scar. The collagen-based composite film is used for various causes of mechanical injuries, burns, scalds, skin ulcers, cosmetic plastic surgery and hemostasis.

Description

technical field [0001] The invention relates to a preparation method of a collagen matrix surface wound repair membrane material with tissue induction, belonging to the field of preparation of medical materials. Background technique [0002] Skin is the natural barrier of the human body, which plays an important role in maintaining the stability of the internal environment and preventing microbial invasion. If the skin is damaged in a large area, it will cause many local or even systemic problems, such as increased metabolism, excessive loss of water and protein, immune system disorders, etc., and even life-threatening. Reconstructing or restoring the skin barrier function is the ultimate goal of body surface wound therapy. Before this goal is achieved, a wound dressing with excellent performance can temporarily function as part of the skin and provide an environment conducive to wound healing, waiting for the epithelialization of the wound or Transition to rebuilding the p...

AI Technical Summary

Problems solved by technology

①Epidermal cell membrane: The disadvantage of this membrane (KC) is that the patient’s skin biopsy specimen must be taken, which requires 2-3 weeks of preparation time, lacks dermal components, and the membrane is thin and fragile [O'ConnerNE, Malliken JB, Bankaclilegcls, et al al. Grafting of burns with cultured epitheliunt prepared from autologous epidermal cells [J]. Lancet, 1981, 1: 75-78]

② Acellular dermal matrix: its disadvantage is that it may contain unclean antigenic substances, which may cause the risk of virus infection, and the material has poor pore penetration

③ Collagen sponge membrane: such as Chinese patents 94118836.1 and 01134743.0, its disadvantage is that the material does not contain cell growth factors, and its mechanical properties are poor, and it cannot resist infection; Chinese patent 200510022581. Microsphere slow-release technology, growth factors are easy to lose biological activity

④ Collagen gel: such as Chinese patents 02117585.3 and 03130382.X, the disadvantage is that the production process is complicated, the collagen gel will shrink by about 80%, the ability to resist collagenase degradation is poor, and it is easy to be infected by viruses and immune rejection. difficult to operate