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Nanometer magnetic fluorescent microsphere and its preparation and application

A fluorescent nano and microsphere technology, applied in the field of biological nanomaterials, can solve the problems of untraceable magnetic nanomaterials, lack of dual-functional magnetic fluorescent nanospheres, etc.

Inactive Publication Date: 2009-03-18
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved in the present invention is to provide a magnetic fluorescent nanosphere and its preparation method and application to overcome the lack of dual-functional magnetic fluorescent nanospheres with both fluorescence and magnetism in the prior art, which cannot be displayed by fluorescent substances. Tracking defects in magnetic nanomaterials

Method used

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  • Nanometer magnetic fluorescent microsphere and its preparation and application
  • Nanometer magnetic fluorescent microsphere and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Eu 3+ Y-Fe 2 o 3 Preparation and preparation:

[0046] 0.2mol / L FeSO 4 .7H 2 O, 0.2mol / L FeCl 2 .6H 2 O and 0.2mol / L EuCl 2 .6H 2 O mixed solution, while stirring, dropwise add 2.5mol / L NaOH solution (final concentration is FeCl 2 : FeSO 4 .: EuCl 2 : NaOH=1:2:2:5). The resulting precipitate was aged at 65 °C for 2.5 h. Filter and repeat with ddH 2 Washed with O, dried at 65°C for 24 hours, and ground in an agate mortar, the product obtained was Eu 3+ Y-Fe 2 o 3 Complex.

[0047] SiO2 wrapped Eu 3+ Y-Fe 2 o 3 :

[0048] Mix Triton X-100, n-hexanol, and cyclohexane at a ratio of 1:1:4, ultrasonically treat it into a uniform, transparent and stable microemulsion, and add 0.1g of Eu 3+ Y-Fe 2 o 3 After ultrasonication for 30 minutes for the complex, take the supernatant and put it in a Erlenmeyer flask, place it in a constant temperature water bath at 25°C and stir for 30 minutes. Then add 1ml of 28% ammonia water dropwise to the system, stir for 30 ...

Embodiment 2

[0054] Implementation Effect

[0055] Eu processed through several steps in Example 1 3+ Y-Fe 2 o 3 The compound is observed by a scanning electron microscope, and it is determined that the particle size of the magnetic fluorescent nanosphere is relatively uniform, and the surface of the particle is smooth. It can be used as a carrier and probe for research. See Figure 1-4 .

Embodiment 3

[0057] Amino-modified Fluorescent Nanoparticles for Nucleic Acid Separation

[0058] Take 1 to 3 mg of the surface-modified amino magnetic fluorescent nanospheres prepared in Example 1, and add them to a phosphate buffer system with a pH of 7.0 to 8.0, and add a crosslinking agent such as glutaraldehyde in an amount of 50 to 200 μL. A mixed solution was formed. The mixed solution is treated with ultrasonic wave for 10-30 minutes. Under appropriate temperature conditions, react for 4 to 6 hours. After centrifuging to remove the supernatant, and then ultrasonically washing with phosphate buffer saline for 2-3 times, the particles were re-dispersed in the phosphate buffer solution.

[0059] Take a certain amount of Oligo(dT), and perform a cross-linking reaction with the above-mentioned microspheres according to a conventional method. The supernatant was removed by magnetic separation, washed 1-2 times with phosphate buffer solution, and finally magnetic fluorescent nanosphere...

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Abstract

The present invention relates to nanometer magnetic fluorescent microsphere and its preparation process and application. The nanometer magnetic fluorescent microsphere is one core-shell including core of Eu3+Y-Fe2O3 compound, shell of SiO2 coated fluorescent material and surface modifying organic functional group. It is prepared through reverse micro emulsion process to synthesize nanometer particle, and the subsequent surface chemical modification to become magnetic nanometer particle capable of combining with probe. The nanometer magnetic fluorescent microsphere is used as biological research tool with integrated fluorescent indication and magnetic separation, and may have important application in biomicromolecule detection, medical diagnosis and other fields.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and more specifically relates to a nanomaterial that can be used in biology. Background technique [0002] Nanotechnology is a cutting-edge and interdisciplinary emerging discipline developed in the late 1980s and early 1990s. It is a technology for studying the law of motion of matter or its characteristics on the scale of 0.1-100nm. Since the goal of this technology is to enable human beings to manipulate the microcosm according to their own wishes and make him serve human beings, it is considered a series of researches in the 21st century. The emergence and development of high technology is one of the hot subjects that have a very important impact, and the rapid development of nanomaterials involves all aspects of human life. [0003] Nanomaterials, also known as nanoparticles (nanoparticles), have some unique effects, showing special characteristics of light, heat, force and magnetism....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02G01N33/52
Inventor 李兴玉李静程呈
Owner SHANGHAI NORMAL UNIVERSITY
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