Microecological preparation-tetragenous viable bacteria preparation and method for preparing same

A live bacteria preparation and live bacteria technology, applied in the field of micro-ecological preparations, can solve the problems of unsustainable effect and single effect

Inactive Publication Date: 2008-08-20
江苏省苏微微生物研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Single-strain type, such as photosynthetic bacteria preparations, bacillus preparations, etc., contains only one kind of microorganism, and has no advantages of synergistic effect of multiple strains. Relatively speaking, there are limitations of single effect and short-lasting effect

Method used

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  • Microecological preparation-tetragenous viable bacteria preparation and method for preparing same
  • Microecological preparation-tetragenous viable bacteria preparation and method for preparing same
  • Microecological preparation-tetragenous viable bacteria preparation and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Example 1: Pure culture of live bacteria

[0117] (1) Pure cultivation of Bacillus subtilis (Bacillus subtilis CGMCC1.1414)

[0118] Strain activation

[0119] Open the freeze-dried strain of Bacillus subtilis aseptically, inoculate it on the nutrient broth agar slant of a test tube with a streak, culture at 37°C for 18-24h, then streak it and transfer it to the nutrient broth agar eggplant bottle slant, cultivate at 37°C for 20-24h , Microscopic examination, when more than 90% of the bacteria form spores, it is mature and ready to transplant.

[0120] Seed culture

[0121] Prepare a sterile conical flask containing glass beads, scrape off the mature bacterial sludge on the slope of the eggplant bottle with sterile water, put it into the conical flask, shake and disperse the bacterial sludge to obtain a uniform bacterial suspension. Heat the bacterial suspension in a water bath at 85°C for 5 minutes, and insert a 5% inoculum volume to 0.1m 3 Seed pot. The filling factor of ...

Embodiment 2

[0156] Example 2: Mixed culture of Clostridium butyricum, Lactobacillus casei and live yeast cells

[0157] Strain activation

[0158] The activation of the lyophilized species of Clostridium butyricum, Lactobacillus casei, and yeast is the same as described in Example 1.

[0159] Seed culture

[0160] The seed culture of Clostridium butyricum, Lactobacillus casei, and yeast is the same as described in Example 1.

[0161] Fermentation tank culture

[0162] The seed culture solution of Clostridium butyricum, Lactobacillus casei, and yeast was inserted into 1m at the inoculum of 6%, 12%, and 2% respectively. 3 Fermentation tank, the filling factor is 80% (v / v).

[0163] The composition of the culture medium of the fermenter is: 20g brown sugar, 5g peptone, 3g yeast extract, KH 2 PO 4 1g, (NH 4 ) 2 SO 4 2g, NaCl 3g, CaCO 3 3g, 3g of NaAc, 1000ml of tap water, pH 7.0, and stand anaerobic culture at 35°C for 48h.

[0164] Bacteria collection

[0165] The culture solution was pumped int...

Embodiment 3

[0166] Example 3: Preparation of pure microbe powder

[0167] (1) Bacillus subtilis original bacteria powder

[0168] Mix the wet cells of Bacillus subtilis collected after pure seed cultivation with dry starch in a mass ratio of 1:2, dry at a low temperature of 40-50℃ for 20-24h, crush with a pulverizer, pass through a 40-mesh sieve, and adjust with dry starch The total number of live bacteria of Bacillus subtilis in the original bacteria powder ≥5×10 8 CFU / gram.

[0169] (2) Clostridium butyricum original bacteria powder

[0170] The wet cells of Clostridium butyricum collected after the pure breed culture are mixed with dry starch in a mass ratio of 1:2.5, dried at a low temperature of 40-50℃ for 20-24h, crushed by a pulverizer, passed through a 40-mesh sieve, and dried with a The total number of viable bacteria of Clostridium butyricum in the starch-regulating original bacteria powder ≥5×10 8 CFU / gram.

[0171] (3) Original Lactobacillus casei powder

[0172] The wet cells of ...

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Abstract

This invention relates to a micro-ecological agent and the preparation method thereof, belonging to the micro-ecological agent technological field. The preparation of the active bacteria agent of this invention comprises: using L-calcii lactas and four useful micro-organisms of clostridium bytyricum, Lactobacillus casei, bacillus subtilis and saccharomycete as characteristic elements, cultured purely or mixedly by means of universal fermentator, producing active bacteria powder by freeze-drying, and acquiring the finished products by double crossing of the powder at a specific proportion. By using the anaerobic and aerobic characteristics of related bacterial strains, this agent has the following merits: playing the synergy effect in enhancing the disease resistance of marine animals and the water purification of aquaculture, substituting antibiotics fully or partly for the disease prevention and cure in fresh water aquiculture and mariculture, and improving the economic benefits of aquaculture. The L-calcii lactase component can accelerate the husking growth and prevent the disease of soft husk of shellfish marine animals like shrimp and crab. This agent is characterized of being nontoxic, free from side effects, nonpersistent, without secondary pollution and no drug tolerance, and will have a wide commercial application in the fresh water aquiculture mariculture.

Description

Technical field [0001] A microecological preparation-quadruple viable bacteria preparation and a preparation method thereof belong to the technical field of microecological preparations. technical background [0002] In recent years, with the rapid development of my country's aquaculture industry, the scale of high-density and intensive aquaculture has been expanding. The high concentration of aquatic animal excrement in the aquaculture water body, the bait residue caused by a large amount of bait feeding and the arbitrary discharge of sewage The pollution of natural waters in China has worsened the ecological environment of breeding. Aquatic animals, especially crustaceans such as shrimps and crabs, have a low immune system. They mainly rely on non-specific substances produced by various blood cells, such as clusterin lysozyme, to exert the body’s non-specific immune function. In the deteriorating aquaculture environment, it is easy to Attacked by pathogenic bacteria and viruses...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/07C12R1/245C12R1/125
Inventor 陆茂林孙梅匡群施大林刘淮何义进
Owner 江苏省苏微微生物研究有限公司
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