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Preparation method of immune nanometer gold test paper strip for fast detecting furazolidone

A technology of furazolidone and nano-gold, which is applied in the field of preparation of immune nano-gold test strips, can solve the problems of time-consuming and manpower, and achieve the effects of easy portability, improved sensitivity, and broad application prospects

Inactive Publication Date: 2008-07-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the deficiencies and limitations of the existing technology for detecting furazolidone, and provides a method for preparing an immune nano-gold test strip for rapid detection of furazolidone, so that the means for detecting furazolidone by using immune nano-gold labeled antibody technology is faster and more sensitive. And it can realize the on-site detection of furazolidone in feed and animal products (meat samples or urine samples), which overcomes the shortcomings of other methods that require special instruments and consume time and manpower.

Method used

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  • Preparation method of immune nanometer gold test paper strip for fast detecting furazolidone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] ① Synthesize furazolidone-bovine serum albumin conjugates and furazolidone-catalase conjugates, and identify the binding ratio of these two conjugates. Furazolidone was made into a 225 μg / ml solution before the identification of the binding ratio, and furazolidone-bovine serum albumin conjugate and furazolidone-catalase conjugate were made into 0.4mg / ml and 0.32mg / ml aqueous solutions respectively , and at the same time prepare 0.4mg / ml bovine serum albumin solution and 0.32mg / ml catalase aqueous solution respectively, and then carry out ultraviolet scanning, according to OD 280 Calculate the respective molar absorptivity ε, measure respectively the binding ratio of furazolidone and bovine serum albumin and the binding ratio of furazolidone and catalase, binding ratio=[ε280 (conjugate)-ε(bovine serum albumin or hydrogen peroxide enzyme)] / ε280 (furazolidone). According to the calculation results, the binding ratios of furazolidone to bovine serum albumin and furazolidon...

Embodiment 2

[0058]① The specific process of synthesizing furazolidone-bovine serum albumin conjugate and furazolidone-catalase conjugate and the method of identifying the binding ratio of these two conjugates are basically the same as in Example 1. The difference is that the dosage of furazolidone was reduced to 56.3 mg when synthesizing the furazolidone-catalase conjugate. The finally obtained binding ratio of furazolidone to catalase is 25:1. When this furazolidone-catalase conjugate was immobilized on the nitrocellulose membrane and carried out the immunogold nanometer test paper test experiment, under the premise of not adding the test sample furazolidone, the degree of color development was not statistically different from that in Example 1. Significant difference; but after adding the sample to be tested, furazolidone, the detection sensitivity is slightly higher than the implementation result of Example 1, but there is no statistically significant difference.

[0059] ② The specif...

Embodiment 3

[0066] ① The specific process of synthesizing furazolidone-bovine serum albumin conjugate and furazolidone-catalase conjugate and the method of identifying the binding ratio of these two conjugates are basically the same as in Example 1. The difference is that when synthesizing the furazolidone-catalase conjugate, the dosage of furazolidone was increased to 225.2 mg. The final binding ratio of furazolidone to catalase was 82:1. When the furazolidone-catalase conjugate was immobilized on the nitrocellulose membrane to perform the immunogold nanometer test strip test experiment, under the premise of not adding the test sample furazolidone, the degree of color development was significantly lighter than that of Example 1. and after adding furazolidone, the sample to be tested, the detection sensitivity also decreased significantly, indicating that the synthesized furazolidone-catalase conjugate and antibody immunoreactivity was not strong, resulting in significant detection accura...

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Abstract

A method for preparing immune nanogold test paper used to quickly detect furazolidone includes synthesizing coupled matter of flurazolidone-cattle seralbumin and coupled matter of flurazolidone-catalase ; using coupled matter of flurazolidone-cattle seralbumin as immune total antigen; making immune experiment on rabbit to obtain multiple clone antibody of rabbit resistance furazolidone; preparing nanogold reagent being used to label said antibody; fixing IgG antibody and coupled matter of furazolidone-catalase on nitrocellulose film; sealing unreacted active group; using above-prepared film, antibody and paper as well as sample pad to form said test paper.

Description

technical field [0001] The invention relates to a method in the technical field of detection, in particular to a method for preparing an immune nano-gold test strip for rapid detection of furazolidone. Background technique [0002] Furazolidone, whose chemical name is 3-(5-nitrofurfurylidene) 2-oxazolidinone, is an antibacterial substance, mainly used to prevent and treat bacterial infections in humans and animals and as an antibacterial and growth-promoting agent for livestock and poultry. agent. However, because the drug can produce a variety of strong side effects in animals and humans, it is banned by some countries such as Europe, America and China. The European Union (EU) N0807 / 2001 regulations stipulate the use of high performance liquid chromatography for the detection of furazolidone, with a detection limit of 2-4ppb; China currently uses the industry standard SN0530-1996, requiring the national standard method for the detection of furazolidone residues in animal-d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/532G01N33/558G01N21/78
Inventor 柴春彦刘国艳
Owner SHANGHAI JIAO TONG UNIV
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