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Bioderived amnion, composite bioderived amnion and its preparation method

An amniotic membrane and biological technology, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve the problems of high cost of trypsin treatment, ineffective removal of antigen components, etc., to shorten skin healing time, promote cell adhesion, The effect of preventing tissue adhesion

Active Publication Date: 2008-02-13
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method of the amniotic membrane graft is "removing cellular substances from human placental amniotic membranes", that is, using trypsin to remove cellular substances, or cross-linking. The use of trypsin to treat the cost is high, and other antigenic components in the material cannot be effectively removed ( Such as antigenic components in cells such as soluble macromolecules and fat-soluble proteins), still have certain antigenicity

Method used

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  • Bioderived amnion, composite bioderived amnion and its preparation method
  • Bioderived amnion, composite bioderived amnion and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of biologically derived amniotic membrane

[0051] Take fresh healthy human amniotic membrane, rinse with normal saline for 3 times, degrease with chloroform / methanol (1:1, v / v) until the supernatant is clear, cross-link with 0.25% glutaraldehyde for 10 minutes, rinse with normal saline for 3 times, each time for 10 minutes; Treat with 0.5% w / v SDS (sodium dodecylsulfonate) for 4 hours, rinse with normal saline 3 times, 10 minutes each time; digest with 0.25% w / v trypsin for 8 hours, rinse with normal saline 3 times, 10 minutes each time , freeze-dried, subpackaged, sealed, and sterilized with ethylene oxide.

Embodiment 2

[0052] Example 2 Preparation of Composite Biologically Derived Amniotic Membrane

[0053] Collagen is extracted from human tendon by acid extraction, and 0.3% acetic acid solution is used to prepare a 0.5% collagen solution. The biologically derived amniotic membrane prepared in Example 1 that has not been lyophilized after treatment is covered in stainless steel containers of different specifications (such as 40x40x8mm ) bottom, add collagen solution according to the required material thickness, freeze at -80°C for half an hour, freeze-dry in a freeze dryer for 24 hours, cross-link in 0.25% glutaraldehyde solution at 4°C for 12 hours, fully rinse with pure water, wash The residual glutaraldehyde was removed, and then freeze-dried to obtain the composite bio-derived amniotic membrane. Pack and seal, and sterilize by ethylene oxide or gamma ray irradiation.

Embodiment 3

[0054] Physical properties of embodiment 3 biologically derived amniotic membrane and composite biologically derived amniotic membrane

[0055] The physical properties of the biologically derived amniotic membrane prepared in Example 1 and the composite biologically derived amniotic membrane prepared in Example 2 are as follows:

[0056] Bioderived amnion is a colorless film that is easily rehydrated and becomes transparent after rehydration (Figure 1). Composite bio-derived amnion is a yellow spongy film whose thickness can be determined according to needs (Figure 2).

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Abstract

The present invention relates to a biological deriving amniotic membrane, composite biological deriving amniotic membrane and method for making same, as a material used for repair engineering of skins, corneas and nervous tissues and clinical application, especially for the biological deriving amniotic membrane treated through a de-cell process and the composite biological deriving amniotic membrane formed by recombination with collagen sponges. The biological deriving amniotic membrane and the composite biological deriving amniotic membrane prepared by adopting the process in accordance with the present invention keep the complete spatial structure of amniotic membrane and entirely remove cells therein, with a very low antigen character. The biological deriving amniotic membrane and the composite biological deriving amniotic membrane in accordance with the present invention are used for defending tissue adhesions in surgical operations, biological dressings, eye surface transplantation and disease treating in tissue engineering skins, corneas, nerves and the like; can guard against tissue adhesions, promote cell adhesions, shorten the time for skin healing, alleviate patient aches, rebuild normal structure of eye surfaces, without leading to immunoreactions and with high security.

Description

technical field [0001] The invention relates to a biologically derived amniotic membrane, a composite biologically derived amniotic membrane and a preparation method thereof. Specifically, it is a biologically derived material obtained from human placental amniotic membrane as a raw material through treatment. It is a skin, cornea, nerve tissue repair project and The materials used in clinical surgery belong to the field of biology. Background technique [0002] The amniotic membrane has five layers: epithelial cell layer, basement membrane, compact layer, fibroblast layer and spongy layer. Studies have shown that amniotic membrane has the following characteristics: 1. Amniotic membrane contains fibronectin (fibernectin, FN), laminin (lamnine, LN), type IV collagen, heparan sulfate proteoglycans (HSPGs) and some other proteoglycan macromolecules and some Cell growth factors such as EGF and BFGF can promote important functions such as cell adhesion, migration, proliferation,...

Claims

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Application Information

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IPC IPC(8): C12N5/08
Inventor 杨志明罗静聪
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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