51results about How to "Selection inefficiency" patented technology

The invention discloses a rapeseed pod number major QTL molecular marker and its application. The marker can be used for marker-assisted selection in pod number character improvement and fine mapping and map-based cloning of QTL site. Primers of the molecular marker are CNU400F:5'-CGAGTTTTTGTGTGTACGTATAGTAAT-3' and CNU400R:5'-CCAAAGTGCGTAAAGGAAGG-3'. Rapeseed Zhongshuang 11 and 73290F3 generation are selected by the use of the marker, and silique number of the screened F3 single plant is higher than that of Zhongshuang 11 by 92.3%. Thus, the marker is utilized for assistant selection so as to greatly enhance selection efficiency of high yield breeding.

The invention relates to the technical field of maize molecular breeding and biology, in particular to a maize rough dwarf disease resistance main-effect QTL, molecular markers closely linked with themain-effect QTL and application of the molecular markers. The main-effect QTL for controlling maize rough dwarf disease resistance is positioned on a maize chromosome 2 for the first time. In a conventional breeding method, identification of the maize rough dwarf disease resistance needs to wait for maize pollinating, the time and labor are wasted, and selection accuracy is poor; and infected single plants can be eliminated in a seedling stage by detecting the main-effect QTL of maize rough dwarf disease resistance, the production cost is saved, and the selection efficiency is greatly improved. The main-effect QTL of maize rough dwarf disease resistance is clear in position and high in contribution rate, the rough dwarf disease resistance character of a maize inbred line material can be remarkably improved, and a detection method is convenient and rapid and is not affected by the environment. The resistance to rough dwarf disease can be predicted by detecting the molecular markers closely linked with rough dwarf disease resistance, so that a rough dwarf disease resistance material is accurately and quickly screened.

The invention relates to a dimensionality reduction method for a hyperspectral image, in particular to a hyperspectral band selection algorithm based on normalized multidimensional mutual information and clone selection. A method for selecting the number of cloning iterations in hyperspectral image band selection is provided. The steps of the present invention are as follows: 1. Read in the hyperspectral image, define the antigen and randomly generate an initial set, and select the best individual composition set according to the individual fitness value. 2. Clone the best individual to generate a temporary clone set, perform high-frequency mutation operation on the clone set, and select the best individual to form a set again. 3. Use the normalized multidimensional mutual information to judge the correlation degree of the two sets before and after to decide whether to stop the iteration. The invention can achieve the purpose of reducing the dimension of hyperspectral images. In order to make the numerical calculation more accurate, the number of iterations is selected by using the normalized multi-dimensional mutual information, which reduces the unnecessary excessive iteration process in the selection process, and is suitable for hyperspectral images. Image band selection application.

The invention discloses a molecular marker of a pot shattering resistance trait major gene locus of rapes and an application. The steps are as follows: 1) hybridizing double 11 variety and 73290 variety (which have obvious differences in the pot shattering resistance traits) in a cabbage type rape varieties, and carrying out selfing on progenies to obtain F2 and F2:3 segregation populations with the segregated pod shattering resistance traits; 2) carrying out polymorphic screening on parent DNA (deoxyribonucleic acid) by utilizing an SSR (simple sequence repeat) primer, and establishing a genetic linkage map by carrying out SSR molecular marker genotyping on the F2 progeny segregation populations; 3) carrying out field experiments and plant inquisition on the F2 and F2:3 segregation populations so as to obtain phenotype data of the pod shattering resistance traits; and 4) carrying out QTL (quantitative trait locus) detection by combining with the developed high-density molecular marker genetic linkage map and genotype and the phenotype data of the segregation populations, utilizing QTL Cart2.5 software to obtain the major gene locus Psr.A9 for controlling the pod shattering resistance of the rapes on a cabbage type rape A9 linkage group and also obtain the molecular marker BrSF0007-39 closely linked with the major gene locus. The selection efficiency of breeding with the pod shattering resistance can be greatly improved by utilizing the marker for assistant selection.

A simple method for screening the wheat genotype able to generate unreduced gamete for breaking through the 'reduplicative bottle neck' of reduplicating monoploid includes such steps as distant hybridizing between wheat and Aegilops variabilis to obtain plant F1, selfing, and screening.

The invention discloses a molecular marker closely linked with a major QTL of a wheat plant height character, and an application of the molecular marker. The invention provides a primer pair for detecting a to-be-detected wheat plant height, which is a primer pair used for amplifying target fragments; the target fragments are fragments including fragments obtained by amplification through a primer1 and a primer 2; the primer 1 is a single-stranded DNA molecule as shown in a sequence 1; and the primer 2 is a single-stranded DNA molecule shown in a sequence 2. An experiment proves that the primer pair SSR-2433 is used for carrying out assisted selection on wheat segregation populations. A result shows that a single plant with a gene type consistent with that of a high-straw parent Jing 411is obviously higher than a single plant with a gene type consistent with that of a dwarf parent, which indicates that the primer pair SSR-2433 is feasible and effective for molecular marker-assisted selection of wheat dwarf breeding.

The invention discloses a seed number per pod character major gene site of rape and application thereof. The process comprises the following steps: (1) hybridizing by Brassica napus variety with obvious difference on each seed number per pod character; (2) carrying out polymorphism screening for parent DNA by public and developed SSR (Simple Sequence Repeat) and SNP (Single Nucleotide Polymorphism) primers, and building a genetic linkage map by molecular mark gene type analysis to an F2-generation segregation population; (3) obtaining the phenotype data of each seed number per pod character by field experiments and variety research to F2 and F2:3 segregation population; and (4) combining the gene type and the phenotype data of the segregation population to carry out QTL (Quantitative Trait Loci) detection. The major gene site qQN.A6 and the molecular marker BrSF50-18 for controlling seed number per pod of rape on an A6 linkage group are obtained. The F3 generation derived from two parents is analyzed by the marker, and an individual plant with the marker is kept. A variety research result shows that the ratio of the seed number per pod is 83.4% higher than the F3 individual plate of F2:F3 family mean value, and thus, the marker is used for assisted selection to greatly improve the selection efficiency of high-yield breeding.

The invention belongs to the technical field of molecular biology and genetic breeding, and specifically discloses a molecular marker primer and application of a main effect gene locus of the number of seeds per corner of rapeseed, through the F2 and F2:3 families of Zhongshuang 11 and 73290 The segregation population was subjected to field experiments and breeding tests to obtain the phenotypic data of the trait of kernel number per corner; combined with the genotype and genetic map of the F2 segregation population, QTL detection was carried out. Obtained the main effect gene loci controlling the number of seeds per corner of rapeseed on the A7 linkage group qSN.A7 and the molecular marker Ni201. Through the genotype analysis of the F3 generation derived from the two parents by this marker, the average number of grains per corner of the selected single plant carrying the favorable gene exceeds the average value of the single plant carrying the unfavorable gene, and the test results show that the single plant carrying the favorable marker has a The proportion of grain number higher than the average value of individual plants carrying unfavorable genes was as high as 88.0%, so the use of this marker for assisted selection can greatly improve the selection efficiency of high-yield breeding.

The invention belongs to the technical field of molecular biology and genetic breeding, and specifically discloses the molecular marker BrSF0239 primer of the main QTL locus in flowering stage and maturity stage of Brassica napus and its application. The phenotype data of flowering period traits were obtained by field experiment and trait investigation of F2:3 family segregation population; combined with the genotype and genetic map of F2 segregation population, QTL detection was carried out, and the joint control of flowering and maturity stages of rapeseed on A2 linkage group was obtained The main effect loci of the rapeseed flowering stage contribute 17.2%, the additive effect is 2.1, and the dominant effect is 0.9; the contribution rate to rape maturity stage is 9.4%, the additive effect is 0.6, and the dominant effect is 0.9. The effect is 0.4. By detecting molecular markers related to maturity traits, the length of maturity can be predicted, and early-maturing varieties can be accurately and quickly screened.

The invention discloses a molecular marker in close linkage with transverse-diameter QTL (quantitative trait loci) of kiwifruit fruits and application of the molecular marker. Primers designed according to the molecular marker are TD-F:5'-GATTGCCCCTTTGCTCTATGA-3' and TD-R:5'-CATGGTCTTGTGCCACCAAT-3. The molecular marker and the application have the advantages that the major effective QTL for controlling the transverse diameters of the fruits are positioned in kiwifruits for the first time, 14.61% of phenotypic variance can be explained, the molecular marker related to transverse-diameter traits of the fruits is detected, and accordingly the transverse diameters of the kiwifruit fruits can be predicted and further can be accurately and quickly screened.