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Linkage molecular markers of maize rough dwarf disease resistance main-effect QTL and application of linkage molecular markers

A technology of molecular markers and crude dwarf disease, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of restricting research results in breeding applications, and the small effect value of a single QTL, so as to achieve detection The method is convenient and fast, improves the anti-rough dwarf traits, and saves production costs

Active Publication Date: 2019-12-31
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] In recent years, domestic and foreign scholars have reported a lot of studies on the location of QTLs for resistance to rough dwarf disease in maize. Multiple resistance QTLs were detected using different populations. These studies have played a certain role in elucidating the genetic mechanism of rough dwarf disease in maize. Depending on the population type and size, genetic background, genetic map density, and statistical analysis method used in the study, there are differences in the number, location, and effect of QTLs mapped to the same trait; and the QTLs that are usually detected are distributed on different chromosomes. The effect size of individual QTLs is small, limiting the application of the findings to breeding

Method used

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  • Linkage molecular markers of maize rough dwarf disease resistance main-effect QTL and application of linkage molecular markers
  • Linkage molecular markers of maize rough dwarf disease resistance main-effect QTL and application of linkage molecular markers
  • Linkage molecular markers of maize rough dwarf disease resistance main-effect QTL and application of linkage molecular markers

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Embodiment 1

[0040] Example 1: Obtaining the linkage molecular markers of the main QTL for resistance to rough dwarf disease in maize:

[0041] (1) Construction of positioning separation groups:

[0042] In the spring of 2013, two experimental sites in Jining City, Shandong Province, planted 199 RIL populations constructed from maize inbred lines 80007 (disease-resistant parent) and 80044 (susceptible parent), and carried out the evaluation of the incidence level of rough dwarf disease under natural inoculation conditions. Phenotype identification. At the same time, 58 RILs were selected to hybridize with 80044 to obtain F 1 , F 1 Continuous selfing for multiple generations as a fine-mapped population.

[0043] (2) DNA extraction:

[0044] Total DNA was extracted from leaves of inbred lines 80007 (disease-resistant parent) and 80044 (susceptible parent) and segregation populations by conventional SDS method (Murray and Thompson 1980).

[0045] (3) Development and synthesis of primers:...

Embodiment 2

[0054] Example 2: Application of molecular markers S33 and D1116 in assisted selection of maize rough dwarf resistance traits

[0055] Its steps include:

[0056] (1) In the spring of 2019, the finely positioned F 8 The population was identified by artificial inoculation (Ren Chunmei, 2018), a single plant was listed and sampled at the seedling stage, and the total DNA of the leaves was extracted, and the qMrdd2 genotype was judged by molecular markers S33 and D1116;

[0057] Those with homozygous band A detected by molecular markers S33 and D1116, and those with one band A and the other heterozygous band or both were detected as resistant strains ;Use molecular markers S33 and D1116 to detect homozygous band type B, and record it as a susceptible individual plant.

[0058] (2) Both artificial inoculation identification and natural disease identification adopt the same disease investigation method. In the maize maturation stage, the disease severity is divided into 5 grades...

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Abstract

The invention relates to the technical field of maize molecular breeding and biology, in particular to a maize rough dwarf disease resistance main-effect QTL, molecular markers closely linked with themain-effect QTL and application of the molecular markers. The main-effect QTL for controlling maize rough dwarf disease resistance is positioned on a maize chromosome 2 for the first time. In a conventional breeding method, identification of the maize rough dwarf disease resistance needs to wait for maize pollinating, the time and labor are wasted, and selection accuracy is poor; and infected single plants can be eliminated in a seedling stage by detecting the main-effect QTL of maize rough dwarf disease resistance, the production cost is saved, and the selection efficiency is greatly improved. The main-effect QTL of maize rough dwarf disease resistance is clear in position and high in contribution rate, the rough dwarf disease resistance character of a maize inbred line material can be remarkably improved, and a detection method is convenient and rapid and is not affected by the environment. The resistance to rough dwarf disease can be predicted by detecting the molecular markers closely linked with rough dwarf disease resistance, so that a rough dwarf disease resistance material is accurately and quickly screened.

Description

technical field [0001] The invention relates to the fields of maize molecular breeding and biotechnology, in particular to a main effect QTL site for resistance to rough dwarf disease of maize, and a molecular marker closely linked with the main effect QTL and its application. Background technique [0002] Corn (ZeaMays.L) is a grain, feed, and energy crop. The global planting area accounts for the first of all major crops, and it plays an extremely important role in ensuring world food, feed, and energy security. Maize rough dwarf is a viral disease with global distribution. Maize rough dwarf disease was first discovered in Italy in 1949 (Biraghi et al., 1949), and then occurred in Argentina, France, Spain, (former) Yugoslavia, Greece, Germany, Iran and other countries to varying degrees (Dovasetal., 2004; Huth et al., 2007; Tao et al., 2013). In 2009, rough dwarf disease has become the most widespread viral disease in Spanish maize production (Achonet al., 2013). Since ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘保申张伟潇徐明良邓穗宁商伟刘庆彩赵燕张彦军张永中徐伟
Owner SHANDONG AGRICULTURAL UNIVERSITY
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