40results about How to "Possesses anti-insect activity" patented technology

The invention provides a Bacillus thuringiensis new bacterial strain HS18-1 with the preserving number CGMCC No.2718. Shown by the toxicity test of HS18-1, the HS18-1 has enormous toxicity towards Lepidoptera and Diptera insets. The Bacillus thuringiensis HS18-1 can be prepared into insecticides for the prevention and control of major crop insects, thus facilitating the diversification and systemization of Bacillus thuringiensis insecticide products, enlarging the usage scope of Bacillus thuringiensis insecticides, reducing the use of agricultural chemical, alleviating environment pollution, and having significant economic value and application prospect.

The invention provides a novel strain BtMC28 of Bacillus thuringiensis, which has a preservation number of CGMCC No.2719. Tests of the virulence activity of the BtMC28 show that the BtMC28 has extremely high virulence on Lepidoptera, dipteral pests, and the like. The Bacillus thuringiensis BtMC28 can be made into a pesticide for controlling major farm crop pests. As a result, the novel strain BtMC28 of Bacillus thuringiensis diversifies and serializes the products of Bacillus thuringiensis pesticides, enlarges the application range of the Bacillus thuringiensis pesticides, reduces the pesticide dosage and environmental pollution, and has significant economic value and application prospect.

The invention provides an insect-resistant gene Cry1F-t and application thereof. A Cry1F gene is completely modified to obtain the Cry1F-t insect-resistant gene as shown by SEQ ID NO: 1; an AdhI enhancer is added in the front of the Cry1F-t insect-resistant gene; the Cry1F-t insect-resistant gene is put on an expression vector including a strong promoter 35S; and the Cry1F-t gene is transferred into a corn genome through an agrobacterium-mediated genetic transformation method to obtain a transgenic material including the Cry1F-t. Proved by an experiment, the Bt toxalbumin modified and synthesized by the invention has an obvious insect killing effect on corn borers; and the Cry1F-t and a target protein expressed by the Cry1F-t can be stably and efficiently expressed in a corn, so that the Cry1F-t and the target protein are used for culturing a Cry1F-t transgenic insect-resistant corn. The Bt insect-resistant gene Cry1F-t can be also applied in the improvement of the insect resistance of other farm crops, fruit trees or vegetables and the like, and an effective approach is provided for solving insect pests in the production of plants such as the corn.

The invention provides an insect-resistant gene Cry1Ab-Material. The synthesis of the gene is as follows: according to the amino acid sequence of the N-terminal of the original Bacillus thuringiensis (Bt) protein (Cry1Ab), the preferred codons of monocotyledon (corn) is used to perform human reformation and synthetize a new Cry1Ab DNA sequence; and the prokaryotic expression vector and plant expression vector are constructed, and transformation is performed in the host cells. Vitro tests prove that the transformed and synthetized Bt gene toxic protein has obvious insecticidal effect on Ostrinia nubilalis. The expression of the insect-resistant gene Cry1Ab-Ma in monocotyledon is stable and efficient, thus the gene can be used to produce insect-resistant transgenic plant.

The invention provides a new Bt protein Cry54Aa1 and an encoding gene thereof. The protein has an amino acid sequence shown in an SEQ ID No.2 or has coordination activity after replacing, deleting and / or increasing one or more amino acids in the amino acid sequence shown in the SEQ ID No.2. The protein can be used for preparing the Bt pesticide, and the gene transformation can be performed to agricultural crops such as cotton, corn, rice, vegetables, and the like so that the agricultural crops have the corresponding binding activities, therefore, the consumption of the pesticide and the environmental pollution are reduced, and the invention has important economic value and application prospect.

The invention provides an insecticidal protein Cryl A. 301 which has 1) an amino acid sequence shown by SEQ ID No.2; or 2) an amino acid sequence which is obtained by substituting the amino acid sequence shown by SEQ ID No.2 or decreasing and or increasing one or more amino acid(s) of the amino acid sequence shown by SEQ ID No.2, and has the same function. The experiment in vitro proves that the reformed and synthesized Bt gene toxin production protein has remarkable insecticidal effect for european corn borer. The insecticidal protein Cryl A. 301 can be efficiently expressed in monocotyledons, thus being used for producing insect-resistant transgenic plants.

The invention provides a novel Bt protein Cry4Cb2 and a coding gene thereof, wherein the protein has an amino acid sequence shown as SEQ ID No.2 or an amino acid sequence which is shown as the SEQ ID No.2, is subjected to substitution, deletion and / or increasing one or more amino acids, and has protein with the same activity. The protein can be used for preparing Bt pesticides, and the gene can convert crops such as cotton, corn, rice, vegetables and the like and make the crops have corresponding anti-insect activity, thereby reducing the using amount of pesticides, reducing environmental pollution, and having important economic value and application prospect.

The invention provides new Bt protein Cry 53Abl and encoding genes thereof. The protein has the amino acid sequences shown in the SEQ ID NO.2 or the protein has the amino acid sequences shown in the SEQ ID NO.2 which are substituted, lacked and / or added with one or a plurality of amino acids and have the same activity. The protein can be used for preparing Bt pesticide. The gene can be transformed into crops such as cotton, corn, vegetables and the like which are caused to have corresponding anti-insect activity, thus reducing the usage volume of the pesticide, reducing environmental pollution and having important economic value and application prospect.

The invention provides an anti-insect protein Cry1A.401, which is characterized by comprising: (1) an amino acid sequence shown as SEQ ID No.2, or (2) an amino acid sequence which is obtained by performing substitution, deletion and / or addition of one or more amino acids on the amino acid sequence shown as SEQ ID No.2 and has equal function. As proved by an in-vitro experiment, a modified and synthesized Bt gene toxoggenic protein has a remarkable insecticidal effect on corn borers. The anti-insect protein Cry1A.401 can be stably and efficiently expressed in monocotyledons, and is further applied to production of anti-insect transgenic plants.

The invention provides a novel Bt protein Cry4Cb1 and a coding gene thereof, wherein the protein has an amino acid sequence shown as SEQ ID No.2 or an amino acid sequence which is shown as the SEQ ID No.2, is subjected to substitution, deletion and / or increasing one or more amino acids, and has protein with the same activity. The protein can be used for preparing Bt pesticides, and the gene can convert crops such as cotton, corn, rice, vegetables and the like and make the crops have corresponding anti-insect activity, thereby reducing the using amount of pesticides, reducing environmental pollution, and having important economic value and application prospect.

The invention provides new Bt protein Cry 30 Fal and encoding gene sthereof. The protein has the amino acid sequences shown in the SEQ ID NO.2 or the protein has the amino acid sequences shown in the SEQ ID NO.2 which are substituted, lacked and / or added with one or a plurality of amino acids and have the same activity. The protein can be used for preparing Bt pesticide. The gene can be transformed into crops such as cotton, corn, rice, vegetables and the like which are caused to have corresponding anti-insect activity, thus reducing the usage volume of the pesticide, reducing environmental pollution and having important economic value and application prospect.

The invention provides a new strain BN23-5 of bacillus thuringiensis. The preservation number is CGMCCNO.9448. By testing the activity of virulence of the BN23-5, it shows that the BN23-5 has extremely high virulence on lepidoptera and the like. The BN23-5 of bacillus thuringiensis can be prepared into insecticide and is used for preventing and treating important crop pests. Thus, bacillus thuringiensis insecticide products can be diversified and serialized, the using range of bacillus thuringiensis insecticide is widened, the using amount of pesticide is reduced, environmental pollution is reduced, and the new strain BN23-5 of bacillus thuringiensis has important economic value and application prospects.

The invention provides a Bacillus thuringiensis new bacterial strain YWC2-8 with the preserving number CGMCC No.2860. Shown by the toxicity test of YWC2-8, the YWC2-8 has enormous toxicity towards Lepidoptera and Diptera insets. The Bacillus thuringiensis YWC2-8 can be prepared into insecticides for the prevention and control of major crop insects, thus facilitating the diversification and systemization of Bacillus thuringiensis insecticide products, enlarging the usage scope of Bacillus thuringiensis insecticides, reducing the use of agricultural chemical, alleviating environment pollution, and having significant economic value and application prospect.

The invention provides a new Bt protein Cry4Cc1 and an encoding gene thereof. The protein has an amino acid sequence shown in an SEQ ID No.2 or has coordination activity after replacing, deleting and / or increasing one or more amino acids in the amino acid sequence shown in the SEQ ID No.2. The protein can be used for preparing the Bt pesticide, and the gene transformation can be performed to agricultural crops such as cotton, corn, rice, vegetables, and the like, so that the agricultural crops have the corresponding binding activities, therefore, the consumption of the pesticide and the environmental pollution are reduced, and the invention has the important economic value and application prospect.

The invention discloses an insect-resistant fusion gene, a protein encoded by the insect-resistant fusion gene, as well as an expression vector and application of the insect-resistant fusion gene; andthe purpose of the invention is to solve the technical problem of irresistible trend of drug resistance of insects to pesticides. According to the invention, an insect-resistant fusion gene and an insect-resistant fusion gene CryAb-VIP3A are designed. The invention also provides a protein encoded by the insect-resistant fusion gene CryAb-VIP3A, and designs an expression vector constructed by using the insect-resistant fusion gene CryAb-VIP3A as well as a recombinant bacterium constructed by using the expression vector. The invention further discloses application of the insect-resistant fusiongene CryAb-VIP3A in preparation of insect-resistant plant cells. The invention still further discloses application of the protein encoded by the insect-resistant fusion gene CryAb-VIP3A in preparation of pesticides. The insect-resistant fusion gene CryAb-VIP3A disclosed by the invention can be stably expressed in corn so as to avoid biological toxicity caused by transferring of an original VIP3A(a) gene into the plant alone and overcome the phenomenon of gene silencing in foreign gene expression, so that more choices are provided for plant pest control; and thus, a new method is provided forkeeping down the trend of the drug resistance of the insects to the pesticides.

The invention provides a new Bt protein Cry30Bal as well as an encoding gene thereof; the protein is provided with an amino acid sequence as shown by SEQ ID No.2 or the protein which has the same activity and is obtained by substituting, deleting and / or adding one or plural amino acid to an amino acid sequence as shown by SEQ ID No.2. The protein of the invention can be used for preparing Bt insecticides, the gene can be used for converting cotton, maize, rice, vegetables and other crops to ensure that the crops have corresponding anti-insect activity, thereby reducing the use of agricultural chemical, alleviating environment pollution, and having significant economic value and application prospect.

The invention provides a new Bt protein Cry56Aal as well as an encoding gene thereof; the protein is provided with an amino acid sequence as shown by SEQ ID No.2 or the protein which has the same activity and is obtained by substituting, deleting and / or adding one or plural amino acid to an amino acid sequence as shown by SEQ ID No.2. The protein of the invention can be used for preparing Bt insecticides; the gene can be used for converting cotton, maize, rice, vegetables and other crops to ensure that the crops have corresponding anti-insect activity, thereby reducing the use of agricultural chemical, alleviating environment pollution, and having significant economic value and application prospect.

The invention provides a new Bt protein Cry52Bal as well as an encoding gene thereof; the protein is provided with an aminoacid sequence as shown by SEQ ID No.2 or the protein which has the same activity and is obtained by substituting, deleting and / or adding one or plural aminoacid to an amino acid sequence as shown by SEQ ID No.2. The protein of the invention can be used for preparing Bt insecticides, the gene can be used for converting cotton, maize, rice, vegetables and other crops to ensure that the crops have corresponding anti-insect activity, thereby reducing the use of agricultural chemical, alleviating environment pollution, and having significant economic value and application prospect.

The invention provides an insect-resistant gene Cry1Ab-t and an encoding protein thereof. In the gene, by using a plant bias codon and reducing a sequence rich in AT, an inverted repeat sequence and a subsequence-containing undefined eucaryotic deoxyribose nucleic acid (DNA) sequence in the original DNA sequence on the premise of keeping part of amino acid sequences of a Bacillus thuringHansis (Bt) protein Cry1Ab unchanged, a new Cry1Ab-t gene sequence is synthesized by artificial modification, then a prokaryotic expression carrier and a plant expression carrier are constructed for the gene, and a corresponding host cell is converted. In vitro experiments prove that a synthesized Bt toxoprotein has an obvious insecticidal effect on corn borers. The insect-resistant gene Cry1Ab-t and a target protein expressed by the gene can be steadily and efficiently expressed in corns, so that the gene can be used for culturing Cry1Ab-t transgene insect-resistant corns. The artificially synthesizedBt insect-resistant gene Cry1Ab-t can be also used for improving the insect resistance of other crops, fruit trees, vegetables and the like.

The invention discloses artificially synthesized insect-resistant protein mCry1Ia2. The invention also discloses a nucleic acid molecule of the protein, and a recombinant vector, a host cell or a recombinant bacterium of the nucleic acid molecule. The invention also discloses applications of the protein, the nucleic acid molecule and the recombinant vector of the nucleic acid molecule, the host cell or the recombinant bacteria to regulation and control of plant insect resistance, insect killing or improvement in insect resistance of transgenic plants, and preparation of insecticides or insectcontrol preparations. In vitro experiments show that the modified Bt toxin-producing protein has higher insecticidal activity against ostrinia nubilalis, and has significant insecticidal effect on thepopulation of ostrinia nubilalis resistant to Cry1Ab, Cry1F and Cry1Ie toxin protein. A novel plant expression vector is constructed by a constitutive promoter, and the insect-resistant gene mCry1Ia2can be stably and efficiently expressed in monocotyledonous plants to be further used for producing the insect-resistant transgenic plants.

The invention provides a Bacillus thuringiensis new bacterial strain HS18-1 with the preserving number CGMCC No.2718. Shown by the toxicity test of HS18-1, the HS18-1 has enormous toxicity towards Lepidoptera and Diptera insets. The Bacillus thuringiensis HS18-1 can be prepared into insecticides for the prevention and control of major crop insects, thus facilitating the diversification and systemization of Bacillus thuringiensis insecticide products, enlarging the usage scope of Bacillus thuringiensis insecticides, reducing the use of agricultural chemical, alleviating environment pollution, and having significant economic value and application prospect.

The invention provides a new Bt protein Cry30Bal as well as an encoding gene thereof; the protein is provided with an amino acid sequence as shown by SEQ ID No.2 or the protein which has the same activity and is obtained by substituting, deleting and / or adding one or plural amino acid to an amino acid sequence as shown by SEQ ID No.2. The protein of the invention can be used for preparing Bt insecticides, the gene can be used for converting cotton, maize, rice, vegetables and other crops to ensure that the crops have corresponding anti-insect activity, thereby reducing the use of agriculturalchemical, alleviating environment pollution, and having significant economic value and application prospect.

The invention provides a new Bt protein Cry4Cc1 and an encoding gene thereof. The protein has an amino acid sequence shown in an SEQ ID No.2 or has coordination activity after replacing, deleting and / or increasing one or more amino acids in the amino acid sequence shown in the SEQ ID No.2. The protein can be used for preparing the Bt pesticide, and the gene transformation can be performed to agricultural crops such as cotton, corn, rice, vegetables, and the like, so that the agricultural crops have the corresponding binding activities, therefore, the consumption of the pesticide and the environmental pollution are reduced, and the invention has the important economic value and application prospect.

The invention discloses an artificially-synthesized Bt insecticidal protein gene Cry1Ab-GM1 and an expression vector and application of the gene, and aims at solving the technical problems that an insect-resistant gene is subjected to loss of expression in corn, and transgenic corn is low in insect resistance. The artificially-synthesized Bt insecticidal protein gene Cry1Ab-GM1 is designed, the eukaryotic expression vector used for genetic transformation of plants is further designed, and the vector comprises an above gene sequence. An engineering bacterium used for genetic transformation of the plants is constructed and comprises the above gene sequence. The artificially-synthesized Bt insecticidal protein gene and the eukaryotic expression vector and the engineering bacterium thereof areapplied to transgenic plants. The artificially-synthesized Bt insecticidal protein gene Cry1Ab-GM1 is provided for the first time, can express in the corn and significantly provide the insect resistance for corn leaves and has wide application space and a wide market prospect in the aspect of providing the insect resistance of plants.

A novel Bt protein Cry4Cb2 and a gene encoding the protein are disclosed by the present invention. Said protein is 1) a protein consisting of the amino acid sequence represented by SEQ ID NO.2, or 2) a protein derived from the protein of 1) by substitution, deletion and / or addition of one or several amino acids in the amino acid sequence represented by SEQ ID NO.2 and having equivalent activity.

The invention provides a new Bt protein Cry56Aal as well as an encoding gene thereof; the protein is provided with an amino acid sequence as shown by SEQ ID No.2 or the protein which has the same activity and is obtained by substituting, deleting and / or adding one or plural amino acid to an amino acid sequence as shown by SEQ ID No.2. The protein of the invention can be used for preparing Bt insecticides; the gene can be used for converting cotton, maize, rice, vegetables and other crops to ensure that the crops have corresponding anti-insect activity, thereby reducing the use of agriculturalchemical, alleviating environment pollution, and having significant economic value and application prospect.

The invention provides a new Bt (Bacillus thuringiensis) protein Cry70Ba1 and an encoding gene thereof. The protein has the amino acid sequence shown in SEQ ID No.1 or the amino acid sequence which is formed by substituting, deleting and / or adding one or more amino acids to the amino acid sequence shown in SEQ ID No.1 and has the same function; and the encoding gene of the protein has the nucleotide sequence shown in SEQ ID No.2. The Cry70Ba1 protein can be used for preparing Bt insecticide; and the encoding gene of the protein can be used for transforming cotton, corn, rice, vegetable and other crops, so that the crops have corresponding anti-insect activity, thus the pesticide consumption is reduced, the environmental pollution is reduced and the Bt protein Cry70Ba1 and the encoding gene have important economic value and application prospects.

The invention provides a new strain BN23-5 of bacillus thuringiensis. The preservation number is CGMCCNO.9448. By testing the activity of virulence of the BN23-5, it shows that the BN23-5 has extremely high virulence on lepidoptera and the like. The BN23-5 of bacillus thuringiensis can be prepared into insecticide and is used for preventing and treating important crop pests. Thus, bacillus thuringiensis insecticide products can be diversified and serialized, the using range of bacillus thuringiensis insecticide is widened, the using amount of pesticide is reduced, environmental pollution is reduced, and the new strain BN23-5 of bacillus thuringiensis has important economic value and application prospects.