The invention relates to an automatic pressure adjustment type pulse signal acquisition device and method based on multiple sensors. The acquisition device comprises a base, a wrist support, a slideable adjusting mechanism, a sensor fixing mechanism supporting column, a sensor fixing mechanism, a stepping motor driver, a stepping motor, pulse sensors, a signal preprocessing circuit, a single-chip microcomputer and a computer, wherein the wrist support and the slideable adjusting mechanism are arranged on the base; the sensor fixing mechanism is arranged on the slideable adjusting mechanism through the sensor fixing mechanism supporting column; the pulse sensors comprise a cun position pulse sensor, a guan position pulse sensor and a chi position pulse sensor, which are independently connected onto the sensor fixing mechanism respectively; the signal preprocessing circuit, the single-chip microcomputer and the computer are sequentially connected, and the single-chip microcomputer, the stepping motor driver, the stepping motor and the sensor fixing mechanism are sequentially connected. Compared with the prior art, the automatic pressure adjustment type pulse signal acquisition device and method based on multiple sensors have the advantages that the measured signals are accurate, the automatic search of the best pulse feeling force is realized and the like.