The invention relates to the technical field of tray disinfection for surgical operation, and in particular to a working method of an anti-sticking double-rotation tray disinfecting device for a surgical operation. The device is characterized in that a rotating rod on the left side is rotationally connected with a box body, the rotating rod on the left side leftwards penetrates through the box body to be connected with a motor, and the motor is fixed on the box body. In this way, a tray is immersed into a disinfectant, so that hands are prevented from being in contact with the disinfectant, amotor switch is started, the motor drives the rotating rod on the left side to rotate, bristles on the two sides extrude the tray, a supporting plate is in an arc shape, so that the tray is driven torotate on the supporting plate, stirring blades are arranged at the right end of a rotating disc on the right side, the resistance is increased, the speed difference is generated between the rotatingdisc on the right side and the rotating disc on the left side, then the cleaning effect of the tray is improved, the stirring blades drive the disinfectant to flow, so that the cleaning effect is improved, the motor switch is turned off after cleaning is completed, a box cover is opened, the tray is jacked up by the supporting plate, hand contact is avoided, so that the hands are well protected, the tray is lifted up, a hot air blower switch is turned on, then the tray can be blow-dried through a blowing plate, the use is convenient.