A desert dry and semi-dry turf transplantation and re-laying method suitable for roadbed construction is that turf transplantation is performed before roadbed earthwork construction, turf re-laying is performed after the roadbed earthwork construction, and finally turf maintenance is performed. The turf transplantation step further includes turf selection, turf excavation, moving and flat laying, temporary planting and watering fertilization operations, and after earthwork activities and infrastructure are finished, the turf re-laying step further includes re-laying face leveling, temporary planted turf transportation, re-laying face fertilization watering and fertilization turf joint filling operations. The final turf maintenance step includes grazing prohibition, enclosure and insect and disease prevention. Soil seed bank resources existing in an original turf are fully utilized, the turf survival rate is high, the regeneration capacity is good, the construction speed can be improved, a construction period can be shortened, an ecological environment in a construction region can be quickly recovered, and resources are saved. The desert dry and semi-dry turf transplantation and re-laying method is simple and convenient to operate, low in maintenance cost and remarkable in revegetation restoration effect, can obtain high biological diversity and landscape naturalness, has very high cost performance and is beneficial for popularization and application.