Method for accurately simulating response of grassland soil seed bank to soil temperature increase
A soil seed bank and grassland technology is applied in the field of simulating the response of soil seed banks to soil temperature increase, which can solve the problems of uneven soil temperature changes, not easy to stabilize, and cannot accurately quantify the temperature increase range, and achieves convenient operation and tools. Simple, powerful results
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specific Embodiment approach 1
[0026] Specific implementation mode one: the method for accurately simulating the response of grassland soil seed bank to soil warming in this implementation mode is carried out according to the following steps:
[0027] 1. Sampling of the soil seed bank: In the selected grassland sample plot, select a sampling point, dig soil with a side length of 5-20cm and a depth of 5-30cm, or dig a soil body with a diameter of 5-20cm and a depth of 5 For a soil column of -30cm, fully mix 3-20 soil columns or soil bodies as one repetition, take a total of 5-10 repetitions, and clean the soil samples;
[0028] 2. Punching holes in the germination vessel: select a cylindrical flowerpot or a conical flowerpot, mark the first point with a marker pen at a distance of 7cm from the upper port of the flowerpot, and mark the second point at a horizontal distance of 3cm from the first point. On the other side of the pot that is symmetrical to the first point and the second point, mark the third poin...
specific Embodiment approach 2
[0037] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the cleaning of the soil sample in step 1 is to remove the plant rhizomes and stone impurities in the soil sample. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0038] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the size of the cylindrical flower pot in step 2 is: 20-25 cm in diameter and 18-20 cm in height. Others are the same as in the first or second embodiment.
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