Composition containing arazyme for the prevention and treatment of cancer

a cancer and arazyme technology, applied in the direction of drug compositions, peptide/protein ingredients, chewing gum, etc., can solve the problems that patients with cancers who have been treated by chemotherapy have suffered serious side effects of systemic chemotherapy, cannot be properly treated by surgical operation, and cannot be properly treated by surgery

Inactive Publication Date: 2009-12-24
INSECT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015]Arazyme of the present invention produced by Aranicola proteolyticus reduces the expressions of MMP-9, NF-κB, p21, PCNA (Proliferating Cell Nuclear Antigen), VEGF (Vascular Endothelial Growth Factor), BCl2 (B-cell CLL / lymphoma 2), p-p38, PKC (Protein Kinase C) and MMP-1 (Matrix MetalloProteinase-1) which are involved in the growth, differentiation, proliferation and metastasis of tumor cells, but increases the expression of catalase playing a role in inhibiting tumor development.

Problems solved by technology

However, a tumor in vertebra or dispersive tumor like leukemia cannot be properly treated by the surgical operation.
Though, patients with cancers who have been treated by chemotherapy have seriously suffered from the side effects of systemic chemotherapy.
Motion sickness and vomiting are common but serious examples of all.
The side effects of chemotherapy can even affect the life of a patient since they might drop the adaptability of a patient rapidly.
So, side effects of chemotherapy and radiotherapy are the biggest problem for the treatment of cancer patients.

Method used

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  • Composition containing arazyme for the prevention and treatment of cancer
  • Composition containing arazyme for the prevention and treatment of cancer
  • Composition containing arazyme for the prevention and treatment of cancer

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Arazyme

[0119]To prepare arazyme, the active ingredient of the present invention, Aranicola proteolycius HY-3 (KCTC 0268BP) was cultured in a culture medium (bacto-trypton 0.5%, yeast extract 0.5%, sodium chloride 0.1%, potassium chloride 0.05%, calcium chloride 0.02%, magnesium sulfate 0.02%) at 22° C. for 18 hours. The culture solution was filtered by membrane filtration (2 μm filter, Satorius, USA) to separate supernatant from the cells. The supernatant was concentrated by membrane filtration (10 kDa Membrane filter, Pall sept, PALL Corporation, USA). Arazyme of the invention has the characteristics of anion. So, the concentrated solution was purified by ion exchange resin (Sigma USA) using DEAE-cellulose (Sigma, USA) pre-treated with 50 mM tris-HCl buffer (pH 7.6) and gel filtration exchange resin using Sephadex G-75 (Sigma USA) pre-treated with 20 mM tris-HCl buffer (pH 7.6). The purified enzyme solution was electrophoresed on 10% SDS-PAGE (Sodium dodecyl sulfate-...

example 2

Arrangement of Experimental Mice and Administration

[0120]5-week old specific pathogen (SPF) free CAnN.Cg-Foxnlnu / CrljBgi line nude mice (15 g, Orient, Korea) were used in this invention.

[0121]The mice were inspected and adapted for 7 days in an animal laboratory. During the adaptation, general symptoms were observed and only healthy animals were selected for the experiment.

[0122]The mice were adapted and raised in an animal laboratory equipped with automatic temperature / humidity regulator by which temperature was set at 22±3° C. and the relative humidity was regulated to be 55±10% and light interval was set at 12 hours (light on at 08:00 and light off at 20:00). The environmental conditions were checked regularly (once every three months). From environmental checkup, any environmental changes that might affect the experiment did not detected. During the whole experimental period, the mice were accommodated less than 5 per each polycarbonate cage [240 W×390 L×175 H (mm)]. For individ...

example 3

Cell Culture

[0125]In the present invention, an estrogen receptor negative human breast cancer cell line MDA-MB-231 (HTB-26) provided from ATCC (American Type Culture Collection, USA) distributed from ATCC was used.

Cell Culture Conditions

[0126]The human breast cancer cell line (MDA-MB-231) was subcultured every 2-3 days in DMEM supplemented with 10% FBS (fetal bovine serum) and 1% ampicillin in a 37° C. 5% CO2 incubator. Sterilized culture dishes were used for the experiment.

Arazyme Treatment

[0127]The human breast cancer cell line (MDA-MB-231) was inoculated in a 6-well dish (6×105 cells / well), followed by culture at 37° C. for overnight. The medium was replaced with a starvation medium containing 1% FBS 4 hours before arazyme was treated, resulting in cell cycle arrest. Arazyme was diluted in sterilized injectable solution at the concentration of 60 μg / ml, which was treated to the culture dish. The culture dish was cultured again in a 37° C. incubator for further 34 hours.

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Abstract

The present invention relates to a pharmaceutical composition for the prevention and treatment of cancer comprising arazyme as an active ingredient. More precisely, when arazyme produced by Aranicola proteolycius was administered to the nude mice transplanted with human lung carcinoma cell line, weight gaining, inhibition of tumor cell growth and infiltration, and down-regulations of MMP-9, NF-κB and PCNA, were observed. In addition, when arazyme was treated to human breast cancer cells (MDA-MB-231), down regulations of p21, PCNA (Proliferating Cell Nuclear Antigen), VEGF (Vascular Endothelial Growth Factor), BCl2 (B-cell CLL / lymphoma 2), p-p38, PKC (Protein Kinase C) and MMP-1 (Matrix MetalloProteinase-1) which are involved in tumor cell growth, differentiation, proliferation and metastasis, were observed along with the up-regulation of catalase which inhibits tumor development. Therefore, the arazyme can be effectively used as a pharmaceutical composition for the prevention and treatment of cancer.

Description

TECHNICAL FIELD[0001]The present invention relates to a composition for the prevention and treatment of cancer which comprises arazyme as an active ingredient, more precisely a composition for the prevention and treatment of cancer comprising arazyme that is produced by Aranicola proteolyticus. BACKGROUND ART[0002]A tumor is developed by uncontrollable disordered abnormal cell proliferation. If this tumor shows a destructive growth, invasiveness and metastasis, it is regarded as a malignant cancer. Invasiveness is a character to infiltrate or destroy surrounding tissues, and in particular, a basal layer forming a boundary of tissues is destroyed by the character, resulting in the local spread and sometimes inflow of a tumor through circulatory system. Metastasis means the spread of tumor cells from the original birthplace to other areas through lymphatic or blood vessels. In a broad sense, metastasis also means the direct extension of tumor cells through serous body cavity or other ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/48A61P35/00A23L13/40A23L23/00
CPCA23C15/065A23G1/423A23G3/366A23G4/123A23G9/363A61K38/4886A23L1/31427A23L1/3149A23L1/39A23L2/02A23L2/66A23L1/3056A23L33/19A23L13/424A23L13/48A23L23/00A61P35/00A61P43/00
Inventor PARK, HO-YONGSON, KWANG-HEESHIN, DONG-HAJEONG, KYU-SHIK
Owner INSECT BIOTECH
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