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Fucosyl transferase gene

a technology of fucosyl transferase and polynucleotide coding, which is applied in the direction of transferases, peptide/protein ingredients, immunological disorders, etc., can solve the problems of plant protein induced immunological reactions in the human body

Inactive Publication Date: 2008-09-25
ALTMANN FRIEDRICH +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0032]Furthermore, the invention relates to a biologically functional vector comprising a DNA molecule according to one of the above-described embodiments, or parts thereof of differing lengths in reverse orientation to the promoter. If this vector is transfected in a host cell, an “antisense mRNA” will be read which is complementary to the mRNA of the GlcNAc-α1,3-fucosyl transferase and complexes the latter. This bond will either hinder correct processing, transportation, stability or, by preventing ribosome annealing, it will hinder translation and thus the normal gene expression of the GlcNAc-α1,3-fucosyl transferase.
[0079]The invention also relates to a method of preparing “plantified” carbohydrate units of human and other vertebrate glycoproteins, wherein fucose units as well as GlcNAc-α1,3-fucosyl transferase encoded by an above-described DNA molecule are admixed to a sample that comprises a carbohydrate unit or a glycoprotein, respectively, so that fucose in α1,3-position will be bound by the GlcNAc-α1,3-fucosyl transferase to the carbohydrate unit or to the glycoprotein, respectively. By the method according to the invention for cloning GlcNAc-α1,3-fucosyl transferase it is possible to produce large amounts of purified enzyme. To obtain a fully active transferase, suitable reaction conditions are provided. It has been shown that the transferase has a particularly high activity at a pH of approximately 7, if 2-(N-morpholino)-ethane sulfonic acid-HCl is used as the buffer. In the presence of bivalent cations, in particular Mn2+, the activity of the recombinant transferase is enhanced. The carbohydrate unit is admixed to the sample either in unbound form or bound to a protein. The recombinant transferase is active for both forms.

Problems solved by technology

The immunological reactions triggered in the human body by plant proteins are the main problem in the medicinal use of recombinant human proteins produced in plants.

Method used

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Examples

Experimental program
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Effect test

example 1

[0093]Isolation of the Core-α1,3-Fucosyl Transferase

[0094]All the steps were carried out at 4° C. Mung bean seedlings were homogenized in a mixer, 0.75 volumes of extraction buffer being used per kg of beans. Subsequently, the homogenate was filtered through two layers of cotton fabric, and the filtrate was centrifuged for 40 min at 30000×g. The supernatant was discarded, and the pellet was extracted with solution buffer over night with continuous stirring. Subsequent centrifugation at 30000×g for 40 min yielded the triton extract.

[0095]The triton extract was purified as follows:

[0096]Step 1: The triton extract was applied to a microgranular diethyl amino ethyl cellulose anion exchanger DE52 cellulose column (5×28 cm) from Whatman, which previously had been calibrated with buffer A. The non-bound fraction was further treated in step 2.

[0097]Step 2: The sample was applied to an Affi-Gel Blue column (2, 5×32) column calibrated with buffer A. After washing of the column with this buffe...

example 2

[0127]SDS-PAGE and Isoelectric Focussing

[0128]An SDS-PAGE was carried out in a Biorad Mini-protean cell on gels with 12.5% acrylamide and 1% bisacrylamide. The gels were stained either with Coomassie Brilliant Blue R-250 or Silver. Isoelectric focussing of the fucosyl transferase was carried out on prefabricated gels having a pI range of between 6-9 (Servalyt precotes 6-9, Serva). The gels were stained with silver according to the producer's protocol. For the two-dimensional electrophoresis, lanes were cut out of the focussing gel, treated with S-alkylating reagents and SDS and subjected to an SDS-PAGE, as described above.

[0129]FIG. 2 shows the illustration of an electrophoresis gel of GlcNAc-α1,3-fucosyl transferase, the two-dimensional electrophoresis being indicated on the left-hand side, and the one-dimensional SDS-PAGE being illustrated on the right-hand side. The lane denoted by A is a standard, the lane denoted by B is the GlcNAc-α1,3-fucosyl transferase from the GnGn-Sepharo...

example 3

[0131]Peptide Sequencing

[0132]For sequencing of the protein, bands were cut out of the Coomassie-stained SDS-Polyacrylamide gel, carboxyamidomethylated and cleaved with trypsin according to Görg et al. 1988, Electrophoresis, 9, 681-692. The tryptic peptides were separated with the reverse phase HPLC on a 1.0×250 mm Vydac C18 at 40° C. at a flow rate of 0.05 ml / min, wherein a HP 1100 apparatus (Hewlett-Packard) was used. The isolated peptides were separated with a Hewlett-Packard G1005 A Protein Sequencing System according to the producer's protocol. Furthermore, the peptide mixture was analyzed by Ingel digestion with MALDI-TOF MS (see below).

[0133]FIG. 4 shows the N-terminal sequences of 4 tryptic peptides 1-4 (SEQ ID NO: 5-8). Departing from the first three peptides, primers S1, A2 and A3 were prepared (SEQ ID NO: 9-11).

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Abstract

A DNA molecule is provided which comprises a sequence according to SEQ ID NO: 1 having an open reading frame from base pair 211 to base pair 1740 or having at least 50% homology to the above-indicated sequence, or hybridizing with the above-indicated sequence under stringent conditions, or comprising a sequence which has degenerated to the above-indicated DNA sequence because of the genetic code, the sequence coding for a plant protein having fucosyltransferase activity or being complementary thereto.

Description

[0001]This is a continuation of application Ser. No. 09 / 913,858, filed Aug. 20, 2001, pending, which is a 371 application of International Application No. PCT / AT00 / 00040, filed Feb. 17, 2000, which claims priority to A270 / 99, filed Feb. 18, 1999, all of which are incorporated herein by reference.FIELD OF THE INVENTION[0002]The invention relates to polynucleotides coding for a fucosyl transferase. Furthermore, the invention relates to partial sequences of these polynucleotides as well as to vectors comprising these polynucleotides, recombinant host cells, plants and insects transfected with the polynucleotides or with DNA derived therefrom, respectively, as well as to glycoproteins produced in these systems.BACKGROUND OF THE INVENTION[0003]Glycoproteins exhibit a variety and complexity of carbohydrate units, the composition and arrangement of the carbohydrates being characteristic of different organisms. The oligosaccharide units of the glycoproteins have a number of tasks, e.g. they...

Claims

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Application Information

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IPC IPC(8): C07K14/00C12N15/11A01H5/00A01K67/033A61K38/45A61P37/08A61P43/00C12NC12N5/10C12N9/00C12N9/10C12N15/09C12N15/54C12PC12P19/18C12P19/44C12P21/00
CPCC12N9/1051C12P19/18C12N15/8218C12N15/8201C12P21/005C12N5/10C12N9/00C12N9/10C12N15/11C12N15/52C12P21/00C12Q1/68A61P37/08A61P43/00
Inventor ALTMANN, FRIEDRICHMUCHA, JANLEITER, HARALTGLOSSL, JOSEFSTAUDACHER, ERIKA
Owner ALTMANN FRIEDRICH
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