Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hematopoietic growth factor inducible neurokinin-1 gene and uses thereof

a technology of hematopoietic growth factor and neurokinin, which is applied in the direction of genetic material ingredients, peptides, drug compositions, etc., can solve the problems of stem cells, bone marrow may not produce enough stem cells, breast cancer metastasis to the bone marrow, etc., to reduce the toxicity of normal tissues and increase the effectiveness of chemo- and radiotherapy

Inactive Publication Date: 2008-03-13
RUTGERS THE STATE UNIV
View PDF1 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is about a new gene, its antisense sequence, and the protein and antibodies that are coded for it. These new compositions can be used to treat hyperproliferative diseases like cancer and inhibit the growth of white blood cells. The invention is especially useful for breast cancer. The methods can also be used to prevent and treat lymphoproliferative syndromes and improve the effectiveness of chemo- and radiotherapy. The use of monoclonal antibodies with the invention offers the possibility of delivering therapeutic agents selectively to tumor sites and limiting toxicity to normal tissues.

Problems solved by technology

Typically, cancer is due to failure of the immune surveillance system in an individual.
Breast cancer metastasis to the bone marrow is a clinical dilemma since the prognosis for the patient is generally poor.
However, in the throes of a diseased state, the bone marrow may not produce enough stem cells, may produce too many stem cells or various ones produced may begin to proliferate uncontrollably.
Further complications arise when these stem cells or their associated progenitors are not able to differentiate into the various morphologically recognizable and functionally capable cells circulating in the blood.
These cells are unable to mature and differentiate properly leading to a significant reduction of normal blood cells in the circulation.
The accumulation of blast cells in the bone marrow prevents production of other cell types resulting in anemia and low platelet blood counts.
Traditional methods of treating these B-cell malignancies, which include chemotherapy and radiotherapy, have limited utility due to toxic side effects.
Short-term side effects of chemotherapy may include significant toxicity, extreme nausea, vomiting, and serious discomfort.
The short-term side effects of radiotherapy may include extreme nausea, vomiting, serious discomfort, sterility and infertility.
These side effects may be moderated by reduced dosages, however, this also increases the risk of remission.
However, these procedures are plagued with exorbitant cost and high rates of failure.
It is both difficult and costly to locate a sufficient donor and even when one is located, rejection of the transplanted cells often takes place, which in turn can lead to graft versus host disease.
Most often, these treatments also include a combination of both chemo and radiotherapies, hence, the concomitant risks involved therein would apply here as well.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hematopoietic growth factor inducible neurokinin-1 gene and uses thereof
  • Hematopoietic growth factor inducible neurokinin-1 gene and uses thereof
  • Hematopoietic growth factor inducible neurokinin-1 gene and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

Materials and Methods

[0203] Unless otherwise specified, general cloning procedures, such as those set forth in Sambrook et al., Molecular Cloning, supra or Ausubel et al. (eds) Current Protocols in Molecular Biology, John Wiley & Sons (2000) were used herein.

[0204] Reagents. Hoffman-La Roche (Nutley, N.J.) provided recombinant human (rh)IL-1α. Stem cell factor (rhSCF), rhIL-6, rhIL-11 and alkaline phosphatase (Alk Phos)-conjugated goat anti-rabbit IgG were purchased from R&D Systems (Minneapolis, Minn.). IL-1β and nerve growth factor (NGF) were purchased from Collaborative Research (Bedford, Mass.) and Amersham Life Science (Cleveland, Ohio) respectively. The following was purchased from Sigma (St Louis, Mo.): lsopropyl-D-Thioglactopyranoside (IPTG), SP, FICOLL HYPAQUE, lipopolysaccharide (LPS), Fibronectin-Fragment III-C (FN-IIIC), 12-0-tetradecanoylphorbol diester (TPA), dimethylsulfoxide (DMSO) and cytochemical staining kits for 2-naphthyl-acetate esterase and naphthol AS-D chl...

example 2

Purification of HGFIN from a Prokaryotic Expression Vector

[0224] PCR was used to amplify the coding region of HGFIN, +60 / +1760 (GENBANK accession number AF322909). The following primer pairs were used in the PCR reaction: 5′-CGG GGT ACC ATG GAA TGT CTC TAC TA-3′ (SEQ ID NO:17) (upstream with KpnI linker) and 5′-CCG GAA TTC TCG AAA TTT AAG AAA CT-3′ (SEQ ID NO:18) (downstream with EcoRI linker). The HGFIN-specific sequences are underlined for both the upstream and downstream primers.

[0225] The amplified DNA fragment was cloned into pHAT10, hereafter referred as pHAT10-HGFIN. The vector was transformed into bacteria and HGFIN-HAT induced with IPTG. Induced bacterial cultures (20 ml) were sonicated in 2 ml of 100 mM Tris, pH 6.8, 4% SDS. After this, HGFIN was verified in the cell-free lysates by western blot analysis using 15 μg of total protein and rabbit anti-HAT. Details on the technique for western blot are described herein. The lysates that showed a band at the predicted size of...

example 3

ProteinChip Analyses for HGFIN-SP Interaction

[0226] Before studying the interaction between SP and HGFIN, each protein was profiled by the Surface Enhanced Laser Desorption / Ionization (SELDI) ProteinChip Array technology (Ciphergen Biosystems Inc., Fremont, Calif.). Normal phase (NP1) arrays were used for profiling and preactivated surface arrays (PSi) for HGFIN-SP interaction. For profiling studies, 2 μg of purified HGFIN or 2 μg of SP were spotted directly onto the NP1 arrays. Prior to adding of the proteins, chips were pre-wet with PBS. Arrays were incubated at room temperature until the protein was absorbed, which took approximately 5 to 10 minutes. After this, 0.5 μl of sinapinic acid (SPA) (Ciphergen Biosystems), diluted at 1:50 in 50% acetonitrile and 0.5% trifluoroacetic acid was added to the arrays. Chips were immediately analyzed using linear, time-lag focusing laser desorption / ionization SELDI-time-of-flight mass spectrometer (Model PBS II). Accurate mass was determined ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pHaaaaaaaaaa
temperatureaaaaaaaaaa
Tmaaaaaaaaaa
Login to View More

Abstract

The present invention discloses the cloning of a new cDNA, HGFIN, from stimulated bone marrow stromal cells that was retrieved with a probe specific for the neurokinin-1 (NK-1) receptor. The novel gene, HGFIN, encodes a protein receptor that is involved in the regulation of hematopoietic proliferation and differentiation. HGFIN is implicated in the treatment of hyperproliferative disorders, particularly bone and breast cancer, because it acts to suppress the proliferating cells.

Description

INTRODUCTION [0001] This application is a continuation-in-part application of U.S. patent application Ser. No. 10 / 463,106, filed Jun. 17, 2003, which is a continuation-in-part of U.S. patent application Ser. No. 10 / 039,272, filed Oct. 20, 2001, now U.S. Pat. No. 6,939,955, which claims priority to provisional patent application U.S. Ser. No. 60 / 241,881, filed Oct. 20, 2000, the disclosures of which are incorporated by reference in their entirety herein. [0002] This invention was made with government support by the following Public Health Service grants: HL-54973 and HL-57675 from the National Institute of Health and CA89868 from the National Cancer Institute. The government may own certain rights in the present invention.BACKGROUND OF THE INVENTION [0003] Bone Marrow is the major source of both lymphocytes (immune cells) and erythrocytes in the adult. Among the various cells that constitute the bone marrow are primitive hematopoietic pluripotent stem cells and progenitor cells. An i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K48/00A61P35/00C12N5/08A61K47/48C07K14/72
CPCC07K14/723A61K47/48269A61K47/642A61P35/00
Inventor RAMESHWAR, PRANELA
Owner RUTGERS THE STATE UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com