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Progenitor cells, methods and uses related thereto

a technology of progenitor cells and cells, applied in the field of progenitor cells, can solve the problems of excess sugar accumulation, raised sugar levels, and body's cells not being able to absorb sugar from the blood stream in normal fashion

Inactive Publication Date: 2005-12-01
CURIS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a method for obtaining a substantially pure population of animal stem or progenitor cells from various tissues such as pancreatic, liver, muscle, and pancreatic tissue. These stem cells can be induced to differentiate into pancreatic islet cells and have the ability for self-regeneration and differentiation to pancreatic lineages. The stem cells can be used for various applications such as cell-based therapy for diabetes and other metabolic disorders. The invention also provides markers for identifying and enriching stem cells. The stem cells can be derived from non-neuronal tissue and can be used for research and development purposes.

Problems solved by technology

The cause of the raised sugar levels is insufficient secretion of the hormone insulin by the pancreas.
In the absence of this hormone, the body's cells are not able to absorb sugar from the blood stream in normal fashion, and the excess sugar accumulates in the blood.
Chronically elevated blood glucose damages tissues and organs.
For instance, diabetes is one of the most prevalent chronic diseases in the United States, and a leading cause of death.
Diabetes is also a very disabling disease, because medications do not control blood sugar levels well enough to prevent swinging between high and low blood sugar levels, with resulting damage to the kidneys, eyes, and blood vessels.
The limiting factor in this approach is the availability of an islet source that is safe, reproducible, and abundant.
However, significant problems to overcome are the low availability of donor tissue, the variability and low yield of islets obtained via dissociation, and the enzymatic and physical damage that may occur as a result of the isolation process (reviewed by Secchi et al., 1997; Sutherland et al., 1998).
In addition are issues of immune rejection and current concerns with xenotransplantation using porcine islets (reviewed by Weir & Bonner-Weir, 1997).
A number of diseases or conditions result frown inappropriate levels or inadequate function of blood platelets.
The radiation is lethal to the patient's endogenous bone marrow stem cells.
Currently, these are replaced by transplantation in a procedure fraught with complications.
Adult nerve cells regenerate poorly and nerve death often causes irreparable damage to congnitive and sensorimotor functions.
However, obtaining sufficient fetal tissue is difficult and presents many ethical problems.

Method used

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  • Progenitor cells, methods and uses related thereto
  • Progenitor cells, methods and uses related thereto
  • Progenitor cells, methods and uses related thereto

Examples

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Effect test

example 1

Isolation of Pancreatic Progenitor Cells

Methods

Duct Isolation and Culture

[0178] Pancreata from two litters of 2 week old Sprague-Dawley rat pups were isolated and placed into 10 ml of (1 U / ml in DMEM) Collagenase A (Boehringer-Mannheim, St. Louis) and digested for 40 min at 37° C. in a shaking water bath at 150-175 rpm. The digest was vortexed briefly and washed once with Ca++ / Mg++-free HBSS (Gibco BRL, Grand Island, N.Y.). The pellet was resuspended in HBSS and filtered through a 500 μm mesh (Costar Corning, Cambridge, Mass.) and washed again. The pellet was resuspended to 50 ml in HBSS; 10 ml was transferred to a 10 cm culture plate (Costar Corning) and placed under a dissecting scope. Individual duct fragments were selected by aspiration with a micropipette and transferred to a plate containing medium with serum. The fragments were selected from this plate a second time, transferred to a fresh tube of medium and serum, and washed before being plated. Fragments were cultured ...

example 2

Induction of Pancreatic Progenitor Cell Differentiation

[0205] The monolayer can be grown in the presence of EGF (10 ng / ml) or TGF-a (10 ng / ml) to enhance growth. Induction of differentiation is believed to be cAMP dependent. Agents which induce an increase in intracellular cAMP levels are anticipated to induce differentiation.

[0206] The cocktail DCE (1 μM Dexamethasone, 100 ng / ml Cholera toxin, 10 ng / ml EGF) induces an increase in the number of insulin positive cells in the cultured duct monolayers. FIG. 9 shows the comparison of monolayers treated with DCE+5% FCS versus 5% FCS alone. Ducts were cultured for five days and then treated for an additional 48 hours. Note that there is an approximate 5-fold increase in the total number of insulin positive cells in the culture in response to DCE treatment. The total number of cells in the culture also increases by approximately 20%. The bars represent the average of quadruplicate wells.

[0207] Dexamethasone, Cholera toxin, Forskolin, Di...

example 3

Isolation of Pancreatic Progenitor Cells using Lectin Cell-Surface Marker

[0212] We also set out to identify cell-type specific markers which could be used to isolate / purify pancreatic progenitor cells, or the pancreatic / ductal epithelial which gives rise to such progenitor cells. Amongs the various canidate reagents we tested, we discovered that certain lectins preferentially bound to, and therefor facilitate the isolation of, duct epithelial cells ultimately able to produce pancreatic progenitor cells.

[0213]Arachis hypogaea (Peanut Agglutinin, PNA) is a plant lectin that binds to specific carbohydrate groups on cell surfaces. PNA binds to galactosyl (β-1,3) N-acetyl galactosamine. It was initially selected for study as a beta cell marker (ref: Heald K A, Hail C A, Hurst R P, Kane N, Downing R, Diabetes Res 1991 May; 17(1):1-6, Separation of beta-cells from dispersed porcine pancreas by selective lectin binding); however, in our hands, PNA did NOT label islet cells from rat, but D...

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Abstract

The present invention relates to a substantially pure population of viable pancreatic progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny.

Description

RELATED APPLICATIONS [0001] This application claims priority to U.S. Utility application Ser. No. 09 / 499,362 filed 2 Feb. 2000 and U.S. Provisional Applications: U.S. Ser. No. 60 / 119,576 filed 10 Feb. 1999; U.S. Ser. No. 60 / 142,305 filed 2 Jul. 1999; and U.S. Ser. No. 60 / 171,338 filed 21 Dec. 1999. The specifications of which are incorporated by reference herein.BACKGROUND OF THE INVENTION [0002] Pluripotent stem cells have generated tremendous interest in the biomedical community. With the realization that stem cells can be isolated from many adult tissues has come the hope that cultures of relatively pure stem cells can be maintained in vitro for use in treating a wide range of conditions. Stem cells, with their capability for self-regeneration in vitro and their ability to produce differentiated cell types, may be useful for replacing the function of aging or failing cells in nearly any organ system. By some estimates, over 100 million Americans suffer from disorders that might b...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/074
CPCC12N5/0678C12N2501/01C12N2501/70C12N2501/115C12N2501/39C12N2501/11
Inventor LU, KUANGHUIPANG, KEVINRUBIN, LEE
Owner CURIS INC
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