Establishing method of rat primary liver cancer model

Abstract

The invention relates the method for building mouse primary hepatic carcinoma model. The method comprises the following steps: culturing HepG2 cell in ICR mouse abdominal cavity, pumping ascites, separating cell, making cell suspension, opening mouse abdominal cavity, injecting cell suspension into mouse hepar with microinjector, using 75% alcohol cotton swab to press the hepar, using alpha-cyanoacrylate binding agent to seal pinhole, then using normal saline to wash hepar, closing abdominal cavity. The result displays that the mouse hepar generation rate is 100%, and the natural disappearing rate is 0%. The model can be used to research human primary hepatic carcinoma generation mechanism, and develop antineoplastic.

Description

1. Technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a method for establishing a mouse primary liver cancer model for the purpose of studying the mechanism of occurrence and development of human liver cancer and the development of antitumor drugs. 2. Background technology [0002] Primary liver cancer is one of the common malignant tumors in my country, and its mortality rate is high, ranking third in the death order of malignant tumors. About 110,000 people die from liver cancer every year in my country, accounting for 45% of the death toll from liver cancer in the world. The treatment of liver cancer needs to be improved urgently. An ideal animal model of liver cancer is an indispensable experimental tool for the development of anti-tumor drugs, the mechanism of liver cancer development, interventional therapy and diagnosis of liver cancer. Therefore, the successful production of experimental animal models of l...

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Problems solved by technology

In the experiments of Han et al., the method of subcutaneously implanting solid tumors in mice was adopted, so the tumor tissue blocks transplanted in each experiment could not come from the same mouse body, and the size of the cut tissue blocks was not easy to be consistent, and the contents were not uniform. Contains other non-cancerous cells, which will seriously affect the repeatability and reliability of the experiment, and H22 is a mouse ascites-type liver cancer cell line, and the drug used in this model cannot fully reflect the effect of anti-liver cancer drugs

Li Qi et al. and Shao Chengwei et al. established a rat transplanted liver cancer model by intrahepatic injection of Walker-256 cells. The Walker-256 cell line used in the model can better simulate the expansibility and expansibility of human liver cancer after implantation into the liver. Invasive growth mode, but the cell line is derived from the spontaneous carcinosarcoma of Wistar rat mammary gland, alpha-fetoprotein (AFP) negative, so this model cannot well simulate the occurrence and development of liver cancer, let alone meet the research and development and screening of anti-liver cancer drugs , The research needs of molecular biology of liver cancer

In addition, Wistar rats and SD rats are more expensive and require a larger breeding space, so it is not easy to produce in large quantities

Although the existing human-nude mouse xenograft liver cancer model has a high transplantation success rate, the immune system of nude mice is deficient, which cannot reflect the real situation of normal organisms, and it is impossible to study the role of the immune system in the occurrence of liver cancer and the The impact on the subsequent development of liver cancer cannot simulate the normal metabolism of drugs in the body

In addition, the nude mouse-human liver cancer transplantation model has high technical requirements, low postoperative survival rate, high sterility requirements, difficult feeding, and high price, so the practicability of this model is greatly limited.

Various existing animal models of liver cancer have certain defects and cannot meet the needs of the current biomedical field

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