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Hydrophobia split vaccine for human body

A rabies virus and vaccine technology, applied in the field of virus split vaccine preparation, can solve the problems of inability to further increase antigen content, increase of vaccine side reactions, etc.

Active Publication Date: 2007-02-28
安徽智飞龙科马生物制药有限责任公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this type of vaccine is that it cannot further increase the antigen content to generate a faster and higher immune response in the human body and provide longer-lasting immune protection
The reason is that if the viral antigen content is further increased, the components in the virus particles that have nothing to do with the antigen, especially those allergens, such as viral miscellaneous proteins, viral nucleic acids and lipids, and residual proteins of the tissues or cells in which the virus was cultured, will also follow. Increase, so vaccine side effects increase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Lysis, extraction and purification of rabies whole virus particles and vaccine preparation:

[0034] The Vero cells were cultured at 37°C and passed through four consecutive passages. After the 4th passage cells were cultured for 3 to 5 days, they were washed with phosphate buffer, and the virus titer inoculated was not less than 7.5LgLD 50 / ml of rabies virus fixed virus CTN-1V strain. Then add cell maintenance solution and incubate at 35°C for 24 hours. Replace with fresh cell maintenance solution, and continue culturing for 2 to 4 days according to the above conditions. When the cytopathic effect (CPE) reaches ++ to +++, harvest and combine the virus solution. Add β-propiolactone at a ratio of 1:4000, and place at 22°C for 8 days to inactivate the virus. After passing the inactivation test, it was concentrated by ultrafiltration with a 100KD filter membrane. 10 mg / ml of protamine sulfate was added to the concentrated virus liquid to make the final concentration 0....

Embodiment 2

[0036] Lysis, extraction and purification of rabies whole virus particles and vaccine preparation:

[0037] The Vero cells were cultured at 37°C and passed through four consecutive passages. After the 4th passage cells were cultured for 3 to 5 days, they were washed with phosphate buffer, and the virus titer inoculated was not less than 7.5LgLD 50 / ml of rabies virus fixed virus aGV strain. Then add cell maintenance solution and incubate at 35°C for 24 hours. Replace with fresh cell maintenance solution, and continue culturing for 2 to 4 days according to the above conditions. When the cytopathic effect (CPE) reaches ++ to +++, harvest and combine the virus solution. Add β-propiolactone at a ratio of 1:4000, and place at 22°C for 8 days to inactivate the virus. After passing the inactivation test, it was concentrated by ultrafiltration with a 100KD filter membrane. 10 mg / ml of protamine sulfate was added to the concentrated virus liquid to make the final concentration 0.1 ...

Embodiment 3

[0039] Safety test of rabies virus split vaccine:

[0040] The safety of the rabies virus split vaccine was assessed by abnormal toxicity tests in mice and guinea pigs. Choose clean-grade guinea pigs with a body weight of 250-350 g, use 2 guinea pigs for each vaccine specification, and inject 5.0 ml of rabies virus split vaccine intraperitoneally into each guinea pig, weigh them before and 7 days after immunization, and observe the vaccination reaction at any time. Choose clean-grade mice with a body weight of 18-22 g, use 5 mice for each vaccine specification, and inject 0.5 ml of rabies virus split vaccine into each mouse, weigh them before and 7 days after immunization, and observe the vaccination reaction at any time . The test results are listed in Table 1.

[0041]

[0042] Animal safety tests have shown that rabies virus split vaccines of different specifications prepared with different fixed strains of rabies are free from contamination by exogenous toxic s...

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PUM

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Abstract

The invention discloses a hydrophobia cracking vaccine and preparing method, which comprises the following steps: fixing toxic plant seeded cell, culturing, obtaining virus liquid, cracking the hydrophobia virus particle through chemical method, extracting, condensing, purifying hydrophobia film segment with barb protrusion and base protein, allocating further to obtain the product.

Description

technical field [0001] The invention relates to a rabies split vaccine. Specifically, the present invention relates to a rabies whole virus particle harvested after cell culture is chemically lysed, and the virus envelope (Envelope) fragment containing spike (G glycoprotein spike) and matrix protein (Matrix protein) is extracted, Reconstituted into a rabies split virus vaccine (Rabies split virus vaccine). This split virus vaccine is used to prevent rabies in humans caused by rabies virus infection. The invention also relates to the preparation method of the virus split vaccine. Background of the invention [0002] Rabies is an acute infectious disease caused by rabies virus infection, and is a zoonotic acute infectious disease with natural foci. Rabies virus infects humans mainly through skin wounds and mucous membranes. The incubation period of rabies can range from several days to several years, with an average of 20 to 90 days. The crowd is generally susceptible. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/205A61P31/12
Inventor 薛平
Owner 安徽智飞龙科马生物制药有限责任公司
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