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Process for the production of diglyceride using holoenzyme

A technology of diglyceride and enzymatic method, which is applied in the field of full enzymatic production of diglyceride, can solve the problems of complex equipment, high process cost, bad influence on product quality, etc.

Inactive Publication Date: 2010-09-08
广东润隆再生资源股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This production method requires deep hydrolysis of oils, complex equipment, and high energy consumption during the hydrolysis process. Moreover, the high-temperature hydrolysis process will also lead to unfavorable reactions such as color deepening and fatty acid isomerization, and a large amount of substrates must be esterified. reaction, the process cost is high, and it will also have a negative impact on product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Commercially available canola rapeseed oil 180kg, add 30% water and 1% lipase Lipase F-AP15 (Amano Enzyme Inc. product) to carry out mechanical stirring hydrolysis reaction, reaction temperature 40 ℃, stop reaction when controlling degree of hydrolysis to be 20% . After the reactant was naturally settled for 2 hours, the oil and water were separated, the water phase was discarded, and the remaining water was removed from the oil phase under vacuum at 80°C to obtain a hydrolyzate. The hydrolyzate is separated by molecular distillation. The distillation process is divided into three stages of distillation. The first stage degasses and dehydrates at a temperature of 100°C; the second stage removes most of the fatty acids at a temperature of 180°C; the third stage removes most of the Glyceride, the temperature is 210°C. The high-temperature fraction is then subjected to decolorization and deodorization in a conventional manner to obtain oil composition 1 mainly composed of...

Embodiment 2

[0018] The hydrolysis reaction conditions were the same as in Example 1, the degree of hydrolysis was controlled to be 55%, the hydrolyzed product was separated by molecular distillation, and the high-temperature fraction was then decolorized and deodorized in a conventional manner to obtain the oil composition 3 mainly composed of diglyceride and triglyceride. The cryogenic fraction was composition 4 dominated by free fatty acids and monoglycerides. The conditions of molecular distillation in this example are as follows: the distillation process is divided into three stages of distillation, the first stage degassing and dehydration, the temperature is 80°C; the second stage removes most of the fatty acids, the temperature is 150°C; the third stage removes large Partial monoglyceride, temperature 190°C.

Embodiment 3

[0020] Carry out hydrolysis reaction with soybean oil, lipase adopts Palatase20000L (Novozymes company product), other hydrolysis reaction conditions are the same as embodiment 1, and control hydrolysis degree is 30%, and hydrolyzate is separated through molecular distillation, and high-temperature distillate then carries out decolorization, deodorization in conventional manner , to obtain the oil composition 5 mainly composed of diglyceride and triglyceride, and the low-temperature fraction is composed of composition 6 mainly composed of free fatty acid and monoglyceride. The conditions of molecular distillation in this example are as follows: the distillation process is divided into three stages of distillation, the first stage degassing and dehydration, the temperature is 90°C; the second stage removes most of the fatty acids, the temperature is 160°C; the third stage removes large Partial monoglyceride, temperature 220°C.

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Abstract

This invention is associated with the producing method of diglyceride with enzyme. The steps include: hydrolyze fat or oil using 1, 3 special lipase, the hydrolysis degree is 20 - 55%. After molecular distillation or short path distillation the obtained hydrolysis products can generate the high temperature fractions with the main contents of diglyceride and triglyceride as well as the low temperature fractions with the main contents of fatty acid and monoglycerides. This invention gets the fat or oil catalyzed by specific lipase, and then appropriately hydrolyzes it. The hydrolyzed products contain a lot of diglycerides, but the contents of by-product monoglycerides are very low. This invention also combined the enzymatic hydrolysis and enzymatic deacidification so to increase the output of diglyceride.

Description

technical field [0001] The present invention relates to a method for producing diacylglycerol by enzymatic method. Background technique [0002] Diacylglycerol is a fat molecule with only two fatty acid chains, which is a natural component of oil and an intermediate product of oil metabolism in the body. Studies in recent years have shown that the absorption and metabolism patterns of diglycerides and triglycerides are different. Diglycerides rarely accumulate in the body after consumption, and diglycerides can reduce visceral fat. Diacylglycerol has highly versatile properties and nutritional functions with common edible oils, and diacylglycerol can also be used to produce functional foods with weight loss effects. Hara K. reported in 1993 that diacylglycerol has the function of lowering blood lipids (Ann Nutr Metab 1993; 37:185), which can be used to prevent and treat hyperlipidemia and cardiovascular and cerebrovascular diseases closely related to hyperlipidemia . Digl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/62C12R1/66C12R1/785C12R1/845C12R1/01
Inventor 杨博杨继国王永华乔海强李汉坤
Owner 广东润隆再生资源股份有限公司
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