Method for preparing HAP/beta-TCP structured tissue engineering bone

Abstract

Disclosed is a method for perparation of biological structure HAP / beta-TCP tissue engineering bones, comprising 1.obtaining HAP by using spongy bones of cattle femoral bone lower part as raw material, treating same by removing bone marrow, lipid, and prorein, then rinsing, drying, and high-temperature processing, reacting HAP with NH4H2PO4, high-temperature processing again, and obtaining a biological structure HAP / beta-TCP material support frame; 2.isolating bone marrow stroma stem cells in vitro and seeding same in culture bottles, adding DMEM culture medium, cuturing at 37 DEG C with 5% CO2, changing the culture medium every 2-3 days, digesting cells with parenzyme as cells grow up to 90%, subculturing same, reserving same when the number of bone marrow stroma reaches to 106-107 / ml; 3. rinsing biolofical structure HAP / beta-TCP material support frame with ultrasonic, dropping the subcultured cells on the support frame homogeneously, induced culturing same, obtaining said biological structure HAP / beta-TCP tissue engineering bone when cells fill internal void of support material. The microstructure of disclosed biological structure HAP / beta-TCP tissue engineering bone is completely analogical with spongy bone, facilitates degradation of osteogenesis and support fame materials in vivo which can be controlled, and so accelerating bone recovery.

Description

Technical field: [0001] The invention relates to the fields of medicine and biomedical engineering, in particular to a method for preparing biologically structured HAP / β-TCP tissue-engineered bone Background technique: [0002] For the repair of bone defects caused by various reasons such as complex trauma and curettage of bone tumors, especially the repair of larger defects, the source of bone supply is still an unsolved problem. Due to the limited amount of autologous bone and the possibility of complications such as hematoma, infection, and pain at the donor site, allogeneic bone has potential risks such as immune inflammatory response and disease transmission. Therefore, it is of great significance to study tissue engineered bone as a bone defect repair material. Tissue engineering involves scaffold materials, seed cells and induction or growth factors, among which scaffold materials and seed cells are the key to tissue engineering. [0003] Bio-inorganic materials are...

AI Technical Summary

Problems solved by technology

[0006] In patients with senile osteoporosis, tumors, diabetes, etc., the repair of bone defects after fractures is slow. One of the important reasons is that the number of stem cells differentiated into osteoblasts that is the source of new bone formation in the whole body and / or the defect is greatly reduced. Therefore, it is necessary to develop bone marrow stromal stem cells implanted with biological structure HAP / β-TCP inorganic scaffold materials and be osteogenicly oriented. Induced construction of tissue-engineered bone, which provides both a scaffold for new bone formation and seed cells to speed up the repair of bone defects

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