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Method for purifying salvianolic acid B

A technology of salvianolic acid and salvia miltiorrhiza, applied in the field of extraction and separation and purification of natural products, can solve the problems of difficulty in ensuring drug safety, many separation process steps, and long extraction time with water, so as to avoid degradation and bacterial contamination, and reduce process steps , the effect of reducing the dissolution of impurities

Inactive Publication Date: 2006-12-13
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The disadvantages of the prior art are that the extraction time with water is long, the degradation of salvianolic acid B is more, and soaking in water for a long time, it is easy to be infected with bacteria, and may produce toxic substances
The use of a large amount of organic solvents has the problem of solvent residues, which makes it difficult to ensure the safety of medication, and the more steps in the separation process, the higher the cost and the lower the yield

Method used

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  • Method for purifying salvianolic acid B

Examples

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Effect test

Embodiment 1

[0021] Take 150g of fresh medicinal materials of Salvia miltiorrhiza (with a slice length of about 3cm) and treat them with medium heat for 8 minutes in a microwave field with a frequency of 2450 Hz. 80g of Salvia miltiorrhiza is sun-dried and extracted with 800ml of water. The extract is filtered with gauze and centrifuged at 3000rpm to remove impurities. The centrifugal fluid is placed on a polyamide column (25×600mm). The resin bed volume is 250ml. First use 750ml of water and 250ml. 20% ethanol and 375ml 50% ethanol were eluted to remove impurities, and then salvianolic acid B was eluted with 375ml 70% ethanol. The eluate containing salvianolic acid B was collected and concentrated under reduced pressure and freeze-dried. Salvianolic acid B with a purity of 98.5% was obtained.

Embodiment 2

[0023] Salvia medicinal materials are crushed and passed through a 40 mesh sieve. Take 30g of Salvia miltiorrhiza powder, add 300ml of water, and treat it in a microwave field with a frequency of 2450 Hz for 3 minutes. After filtering with gauze, it is centrifuged at 3000 rpm to remove impurities, and the centrifugal liquid is placed on a polyamide column (25× 600mm), the resin bed volume is 100ml, first use 300ml water, 100ml 20% ethanol and 150ml 50% ethanol to elute and remove impurities, and then use 400ml 70% ethanol to elute the salvianolic acid B, and collect the salvianolic acid The eluate of B is concentrated under reduced pressure and freeze-dried to obtain salvianolic acid B with a purity of 97.5%.

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Abstract

The invention relates the method of extracting purification pellet phenolic acid B from Sage plant, especially extracting purification pellet phenolic acid B from salvia miltiorrhizae. The method comprises the following steps: treating the salvia miltiorrhizae in microwave field for 1-10 minutes, adding water, or disintegrating salvia miltiorrhizae to 10-60 mesh, and adding water, treating it in microwave field for 1- 20 minutes, centrifuging, removing deposition, then carrying out chromatography with polyamide, removing impurity with water and 20-50% alcohol, washing pellet phenolic acid B with 60-80% alcohol, decompressing, thickening, freezing, drying and getting pellet phenolic acid B whose purify is above 95%. The invention has the advantages of simple technology, low cost, high purify and stable quality.

Description

Technical field [0001] The invention belongs to the technical field of extraction, separation and purification of natural products, and relates to a method for extracting and purifying salvianolic acid B from Salvia plants, in particular to a method for extracting and purifying salvianolic acid B from Salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza bunge is the dried root and rhizome of Salvia miltiorrhiza bunge. It is a perennial herb. It is one of the commonly used medicines in traditional Chinese medicine. It has the effects of removing blood stasis and relieving pain, promoting blood circulation, clearing the heart and removing troubles. Salvia miltiorrhiza is mainly used to treat kidney disease, liver disease, cardiovascular and cerebrovascular diseases, etc., and has a wide range of applications. [0003] Salvianolic acid B (Salvianolic acid B or Lithospermic acid B) is about 2-8% of the dried medicinal materials of Danshen, and it is one of the main ...

Claims

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Application Information

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IPC IPC(8): C07D307/78A61K31/343A61P1/16A61P13/12A61P9/10A61P17/18
Inventor 修志龙郭永学李晓晖张代佳
Owner DALIAN UNIV OF TECH
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