Combined test kit for bacterial vagina disease
A vaginosis and kit technology, applied in the field of bacterial vaginosis combined assay kits, can solve the problems of poor specificity, inconvenience for users, inconvenience for universal use, etc., and achieves the effects of no equipment, low cost, and comprehensive detection.
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Embodiment 1
[0034] In the three holes of the dry chemical reaction device of embodiment 1, the 3MM grade chromatographic paper of Whatman of phi 4.5mm is equipped with respectively the hydrogen peroxide index pad of carrier, leukocyte esterase index pad and sialidase activity reagent pad;
[0035]Production of hydrogen peroxide indicator pad: soak the above-mentioned carrier in the following immersion solution; the immersion solution is prepared by peroxidase 1.2U / m 2 , pH=4.6, 100mmol / L sodium phosphate buffer, 4-aminoantipyridine 5g / m 2 , 3,5 dichlorodihydroxybenzenesulfonic acid 6.7g / m 2 and Vitamin C 2.7g / m 2 composition;
[0036] Production of leukocyte esterase index pad: soak the above carrier in the following immersion solution; the immersion solution is composed of 5 bromo-4 chloro-3 indole acetate 2 and sucrose 15% composition;
[0037] Production of sialidase activity reagent pad: 5 bromo-4 chloro-3 indole neuraminic acid salt 138mg / m 2 , pH=5.2, 200mmol / L sodium acetate b...
Embodiment 2
[0047] In the three holes of the dry chemical reaction device of embodiment 2, the 3MM grade chromatographic paper of Whatman of φ 4.5mm is equipped with respectively the hydrogen peroxide index pad of carrier, leukocyte esterase index pad and sialidase activity reagent pad;
[0048] Production of hydrogen peroxide indicator pad: soak the above-mentioned carrier in the following immersion solution; the immersion solution is prepared by peroxidase 1.8U / m 2 , pH=4.6, 100mmol / L potassium phosphate buffer, 4-aminoantipyridine 7.5g / m 2 , 3,5 dichlorodihydroxybenzenesulfonic acid 10.05g / m 2 , vitamin C 2.7g / m 2 ;
[0049] Production of white blood cell esterase indicator pad: soak the above carrier in the following immersion solution; the immersion solution is composed of 5 bromo-4 chloro-3 indole acetate 2 , sucrose 12%;
[0050] Preparation of sialidase activity reagent pad: soak the above-mentioned carrier in the following immersion solution; 2 , pH=5.2, 200mmol / L potassium ...
Embodiment 3
[0055] In the three holes of the dry chemical reaction device of embodiment 3, the 3MM grade chromatographic paper of Whatman of phi 4.5mm is equipped with respectively the hydrogen peroxide index pad of carrier, leukocyte esterase index pad and sialidase activity reagent pad;
[0056] Production of hydrogen peroxide indicator pad: soak the above-mentioned carrier in the following immersion solution; the immersion solution is prepared by peroxidase 1.8U / m 2 , pH=4.6, 100mmol / L sodium phosphate buffer, 4-aminoantipyridine 8.35g / m 2 , 3,5 dichlorodihydroxybenzenesulfonic acid 11.1g / m 2 , vitamin C 2.7g / m 2 .
[0057] Production of white blood cell esterase indicator pad: soak the above carrier in the following immersion solution; the immersion solution is composed of 5 bromo-4 chloro-3 indole acetate 2 , Trehalose 10%.
[0058] Preparation of sialidase activity reagent pad: soak the above-mentioned carrier in the following immersion solution; the immersion solution is compos...
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