Method for separating and purify dermatansulfate and low-molecular heparan sulfate from sodium heparan product
A technology of heparan sulfate and dermatan sulfate, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve the problem that it is difficult to obtain a single component of mucopolysaccharide, and there is no pure dermatan sulfate. Heparan sulfate products come out and other problems, to achieve the effect of simple method
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Embodiment 1
[0022] (1) The raw material is 100g of by-products produced by heparin sodium, the measured optical rotation is -2.5 degrees, and the potency is 28USP u / mg. Dissolve the raw material in 1200ml of water, add 36g of sodium chloride, adjust the pH to neutral, add ethanol until the volume content reaches 36.5%, let stand for 3 hours, collect the precipitate and dehydrate and dry to obtain 55.4g of crude heparan sulfate.
[0023] The measured optical rotation of the crude heparan sulfate was +22.25 degrees, and the anticoagulant potency was 38 USPu / mg.
[0024] (2), add ethanol in the supernatant liquid that step (1) separates gained, make ethanol volume content reach 50%, leave standstill overnight, collect precipitation, add ethanol dehydration, dry, obtain dermatan sulfate crude product 40.9g.
[0025] The measured optical rotation of the dermatan sulfate crude product is -32.75 degrees, and the potency is 8USPu / mg.
[0026] (3), take 5 g of the dermatan sulfate crude product o...
Embodiment 2
[0031] (1) The raw material is 100 g of by-products produced by heparin sodium, the measured optical rotation is +1.55 degrees, and the potency is 30 USPu / mg. Dissolve the raw material in 1200ml of 3% NaCl solution, adjust the pH to neutral, add ethanol until the volume content reaches 36.0%, let stand for 4 hours, collect the precipitate, dehydrate and dry to obtain 44.4g of crude heparan sulfate.
[0032] The measured optical rotation of the crude heparan sulfate was +27.25 degrees, and the anticoagulant potency: 40USPu / mg.
[0033] (2) Add ethanol to the supernatant obtained in step (1) until the volume content reaches 51%, overnight, collect the precipitate, dehydrate with ethanol, and dry to obtain 49.9 g of crude dermatan sulfate.
[0034] The measured optical rotation of the dermatan sulfate crude product is -26.25 degrees, and the potency is 10USPu / mg.
[0035] (3) Take 5 g of crude dermatan sulfate, dissolve it in 45 ml of water, adjust the pH to 2.7, add 0.03 gram o...
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