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Method for high density culture of lactic acid bacteria for kraut

A technology of high-density culture and lactic acid bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem that the protective agent cannot meet the requirements of freeze-drying, and achieve the goal of overcoming damage, high content, and extended logarithmic The effect of growth period

Inactive Publication Date: 2006-10-11
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A single protective agent cannot meet the requirements of freeze-drying

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0010] (1) Seed cultivation

[0011] Pick the bacterial lawn and insert it into 50ml MRS liquid medium (peptone 10g, meat extract 10g, yeast extract 5g, K 2 HPO 4 2g, diammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 801mL, MgSO 4 ·7H 2 O0.58g, MnSO 4 4H 2 (0.25g, pH6.2~6.4, the same below), 37 ℃ constant temperature culture 18h, transfer to the tomato juice culture medium that contains 2% vegetable protein powder with 5% inoculum size then (the concentration of tomato juice is based on 100ml tomato juice Containing 2g of reducing sugar as the standard, the same below), and cultured at 37°C for 18 hours to obtain the seed solution.

[0012] (2) Preparation before vaccination and vaccination process

[0013] Dissolve vegetable protein powder in 3 liters of tomato juice, use 0.2% sodium citrate, 0.2% disodium hydrogen phosphate, and 0.2% sodium dihydrogen phosphate buffered salt solution to adjust the pH value to about 6.3, and pour it into a 5L fermenter; ferm...

example 2

[0021] (1) Seed cultivation

[0022] Pick the lawn and put it into 50ml MRS liquid medium, culture at 37°C for 18h, then transfer it to 210ml tomato juice medium containing 2% plant protein powder with 5% inoculum, and culture it at 37°C for 18h. That is, the seed liquid is obtained.

[0023] (2) Preparation before vaccination and vaccination process

[0024] Dissolve vegetable protein powder in 3 liters of tomato juice, buffer salt solution of 0.3% sodium citrate, 0.3% disodium hydrogen phosphate, and 0.2% sodium dihydrogen phosphate to adjust the pH value to about 6.5, and pour it into a 5L fermenter; The tank is sterilized in an autoclave; the fermenter is cooled to room temperature, and the seed solution is inoculated into the fermenter with an inoculum of 7% above the alcohol flame circle, and the tank pressure is kept at 0.05 MPa with ordinary nitrogen, and stirred Normal temperature fermentation.

[0025] (3) Adding caustic soda

[0026] When the culture solution in...

example 3

[0032] (1) Seed cultivation

[0033] Pick the lawn and put it into 50ml MRS liquid medium, culture at 37°C for 18h, then transfer it to 210ml tomato juice medium containing 2% plant protein powder with 5% inoculum, and culture it at 37°C for 18h. That is, the seed liquid is obtained.

[0034] (2) Pre-plant preparation and inoculation process

[0035] Dissolve vegetable protein powder in 3 liters of tomato juice, buffer salt solution of 0.3% sodium citrate, 0.3% disodium hydrogen phosphate, and 0.1% sodium dihydrogen phosphate to adjust the pH value to about 6.6, and pour it into a 5L fermenter; The tank is sterilized in an autoclave; the fermenter is cooled to room temperature, and the seed solution is inoculated into the fermenter with an inoculum of 7% above the alcohol flame circle, and the tank pressure is kept at 0.05 MPa with ordinary nitrogen, and stirred Normal temperature fermentation.

[0036] (3) Adding caustic soda

[0037] When the culture solution in the ferm...

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PUM

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Abstract

This invention relates to a high cell density cultivation mehtod of a lactic acid bacterium for pickles, wherein: the conventional Lactobacillus plantarum being applied as the seed strain, activated by routine method for enlarging cultivation, inoculated into fermentating culture media prepared by routine method with the characteristic composition of 0.2-0.3% sodium citrate, 0.2-0.3% disodium phosphate, 0.1-0.2% monosodium orthophosphate and pH 6.3-6.6, during the cultivation process, keeping the pH stable between 5.8-6.2 by flowing ammonia soluthion, after 20h post cultivation, adding 500ml-700ml fresh culture meida, centrifuging, adding recombinant protective agent comprising 10-15% skimmed milk, 5-7% glutavene, 2-3% glycerin and 7-8% maltose, and lyophilizing. This method is characterized of high performance and liability, high concentration of active lactic acid bacteria in the culture, and overcoming the damage and death problems of part of microorganism cells during the lyophilizing process.

Description

technical field [0001] The invention relates to a high-density culture method for lactic acid bacteria used in kimchi. Specifically, it refers to the method of adopting a fermentation medium containing buffer salt, adding ammonia water during the culture process to keep the pH value of the culture solution stable, and adding fresh culture medium in the later stage of culture to promote cell growth. Continuous growth is used to realize high-density cultivation of lactic acid bacteria for pickles; the fermented liquid obtained by the method is centrifuged, and a composite protective agent is added to vacuum freeze-dry to obtain lactic acid bacteria freeze-dried powder. Background technique [0002] High-density culture technology generally refers to the culture technology in which the cell density in liquid culture is more than 10 times higher than that of conventional culture, so as to achieve the purpose of improving bacterial enrichment culture. Not only can the culture vol...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
Inventor 董英孙乐六王文兵崔恒林张红印
Owner JIANGSU UNIV
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