Facility vegetable mycorrhiza production method
A production method and technology for mycorrhizal seedlings are applied in the production field of facility vegetable mycorrhizal seedlings, which can solve the problems of poor environmental regulation ability of facilities, serious soil salinization, soil continuous cropping obstacles, etc. Disease tolerance and drought tolerance, the effect of reducing the damage of soil-borne diseases
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[0030] Embodiment: the production method of facility vegetable mycorrhizal seedling
[0031] (1) Propagation of strains and production of bacterial agents: plant susceptible crops cucumber, corn, sorghum or clover by inoculating strains on sterilized organic soil or soil, and cultivate plants to allow mycorrhizal microorganisms to amplify and multiply in root growth The fibrous roots and mycorrhizal spores obtained with high density and high degree of infection can be used for the production of mycorrhizal seedlings as inoculants. (The root mycorrhizal infection rate (F) of susceptible crops is more than 70%, the infection intensity (M) is more than 40%, and the relative mycorrhizal rate (a) is more than 20% as the inoculum standard)
[0032] (2) Disinfection of seedling soil: Sterilization is mainly to eliminate various bacteria in the soil. The method is to put the seedling soil or seedling substrate into an oven at 160°C for 2 hours, then continue to dry at 160°C for 2 hour...
experiment example 1
[0035] Experimental Example 1: Comparison of the propagation effects of inoculating G.m mycorrhizal agents on different substrates (Table 1)
[0036] Utilize cultivated cucumber to inoculate AMF in different cultivation substrates, compare its infection rate, obtain the AMF inoculum agent with good infection rate, its infection effect is shown in table (table 1), can be seen from the table and adopt organic soil to proliferate The mycorrhizal rate of the inoculum is significantly higher than that of the original inoculum purchased in Hungary, so the AMF inoculum propagated with organic soil can be directly used in production tests, followed by the inoculum propagated in the seedling substrate prepared by peat and vermiculite.
[0037] Matrix type
experiment example 2
[0038] Experimental Example 2: Comparison of different AMF bacterial strain seedling raising conditions (Table 2):
[0039] AMF processing
Mycorrhizal infection rate (%)
Ground dry weight
(g)
root dry weight
(g)
total dry weight
(g)
mycorrhizal dependence
AMF(%)
CK
G.m
G.d
B167
B141
G.v
B168
0g
48b
37d
15.6f
45.2c
52.7a
23.3e
2e
3.2a
2.7bc
2.3d
2.8b
3.4a
2.5cd
0.24d
0.51a
0.4bc
0.27d
0.45b
0.52a
0.35c
2.24f
3.71b
3.1c
2.57e
3.25c
3.92a
2.85d
65.6b
38.4d
14.7f
45.1c
75a
27.2e
[0040] The tested strains were Glomus diuphauam (G.d), Glomus mossea (BEG167), Glomus intraradices (BEG141), Glomus etuni-catuml (BEG168), Glomus versiforme (G.v) and Glomus mosseae-2 (G.m).
[0041] Conclusion: From the above experiments, i...
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