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Stable, water soluble chitin derivatives fluorescent quantal-points and prepn. method therefor

A technology of chitosan derivatives and water-soluble chitosan, which is applied in the field of water-soluble chitosan derivative fluorescent quantum dots and their preparation, can solve the problems of hindering the application of quantum dots, reducing fluorescence efficiency, quantum dot biological toxicity and biological problems such as poor compatibility, to avoid toxic side effects, process safety, and meet environmental protection requirements

Inactive Publication Date: 2006-09-13
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, high-quality fluorescent quantum dots are mainly prepared from metal-organic compounds under high temperature and high pressure. If the quantum dots prepared in this oil phase are used in the biomedical field, they must undergo complex hydrophilic modification. The modification process often causes The significant reduction in fluorescence efficiency, and more importantly, the inability to solve the problems of biotoxicity and poor biocompatibility of quantum dots, greatly hindered the application of quantum dots

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: the chitosan quaternary ammonium salt that molecular weight scope is 1-2 ten thousand is dissolved in distilled water and made 5mg / ml aqueous solution, adds 5 * 10 -3 The cadmium acetate aqueous solution of mol / l, reaction 12 hours, gained product is placed in the there-necked flask, seals and ventilates nitrogen protection, high-speed stirring simultaneously adds the sodium sulfide aqueous solution that is freshly prepared and equimolar with cadmium acetate to solution rapidly, continues to stir, Heating and reflux at 80°C for 2 hours, cooling down rapidly, and dialyzing the obtained solution in distilled water for 2 days with a 20,000 dialysis bag to obtain a bright yellow fluorescent CdS quantum dot solution. The solution is centrifuged at a room temperature below 20°C for 20-30 minutes, the centrifugation speed is generally above 15,000 rpm, the supernatant is discarded, and freeze-dried to obtain the corresponding fluorescent quantum dot powder.

Embodiment 2

[0015] Embodiment 2: the carboxymethyl chitosan that molecular weight scope is 1-2 ten thousand is dissolved in distilled water and made 5mg / ml aqueous solution, adds 5 * 10 -3 mol / l lead acetate aqueous solution, react for 12 hours, put the obtained product in a three-necked flask, seal it with nitrogen protection, stir at a high speed and quickly add a freshly prepared aqueous solution of sodium sulfide equal to the molarity of lead acetate to the solution, continue stirring, 100 °C Heat to reflux for 2 hours, cool rapidly, and dialyze the obtained solution in distilled water for 2 days with a 20,000 dialysis bag to obtain a deep red fluorescent PbS quantum dot solution.

Embodiment 3

[0016] Embodiment 3: the chitosan sulfate that the molecular weight scope is 1-2 ten thousand is made 5mg / ml aqueous solution, adds 5 * 10 -3 mol / l cadmium acetate aqueous solution, reacted for 12 hours, the resulting product was placed in a three-necked flask, sealed and ventilated with nitrogen, stirred at a high speed while rapidly adding a freshly prepared aqueous solution of sodium selenium hydride equal to the molarity of cadmium acetate to the solution, and continued stirring , heated to reflux at 80°C for 2 hours, cooled rapidly, and the resulting solution was dialyzed in distilled water for 2 days with a 20,000 dialysis bag to obtain a bright red fluorescent CdSe quantum dot solution.

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Abstract

This invention discloses a stable water soluble shell polysaccharide derivant fluorescence quanta point and its preparation method. The template is the shell polysaccharide derivant, its production method is home position grown in molecular grid form nm micro-crystal, particle diameter is between 2-6nm. The preparation method is that the derivant is dissolved in pure water, metal salt solution is added under agitation condition at house temperature to form complex, chalcogen predecessor solution is high speed agitated added the formed nm semiconductor micro-crystal, it is quanta point, finally high fluorescence efficiency quanta point solution is got after heat reflow and dialysis. The fluorescence point made in neutral water solution can effectively avoid poisonous side effect of organic solvent, the energy needed is large less than ordinary method, and it is specially to combination of bio-molecular. It has great application prospect in cell recognition, organism mark and disease diagnosis fields.

Description

technical field [0001] The invention relates to a stable water-soluble chitosan derivative fluorescent quantum dot used as a fluorescent probe and a preparation method thereof, belonging to the field of biochemistry. Background technique [0002] Fluorescent quantum dots have attracted great attention in recent years due to their narrow emission, no bleaching, and highly stable fluorescence properties. Compared with traditional fluorescent dyes, fluorescent quantum dots have unique advantages in fluorescent probes, biomarkers, and disease diagnosis. At present, high-quality fluorescent quantum dots are mainly prepared from metal-organic compounds under high temperature and high pressure. If the quantum dots prepared in this oil phase are used in the biomedical field, they must undergo complex hydrophilic modification. The modification process often causes The significant reduction in fluorescence efficiency, and more importantly, the inability to solve the problems of biolo...

Claims

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Application Information

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IPC IPC(8): C09K11/06G01N33/50
Inventor 杜予民王小慧
Owner WUHAN UNIV
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