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Chip copying method for DNA computation

A replication method and DNA probe technology, applied in the field of DNA chips, can solve the problem of only replicating a few chips, and achieve the effect of large-scale mechanization

Inactive Publication Date: 2006-08-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a chip replication method for DNA calculation, which overcomes the disadvantages of using the same surface multiple times in the existing surface calculation method and the defects caused by multi-step enzyme reactions. and the defect that only a few chips can be replicated in the chip replication method

Method used

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  • Chip copying method for DNA computation
  • Chip copying method for DNA computation
  • Chip copying method for DNA computation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1, based on the unrestricted replication of the DNA chip of thiol and polyacrylamide gel system

[0013] The first step is to prepare three sets of DNA materials A, B, and C. The A set is used to construct the mother chip. The sequence and length of the C set are the same as those of the A set except for the sulfhydryl label at the end. The sequence and length of the B set are the same as those of the A set. The materials in group A and C are complementary, and they are complementary probes for the materials in group A and C, and the ends of the DNA in group B are labeled with sulfhydryl groups.

[0014] In the second step, the mother chip is constructed with group A DNA materials.

[0015] The third step is to use group B probes to hybridize with the mother chip at 25-50° C. for half an hour, and then wash.

[0016] The fourth step is to pour the polymerization reaction solution system of acrylamide monomer and its crosslinking agent on the mother chip area...

Embodiment 2

[0021] Embodiment 2, based on the unrestricted replication of the DNA chip of thiol and polyacrylamide gel system

[0022] The first step is to prepare three sets of DNA materials A, B, and C. The A set is used to construct the mother chip. The sequence and length of the C set are the same as those of the A set except for the sulfhydryl label at the end. The sequence and length of the B set are the same as those of the A set. The materials in group A and C are complementary, and they are complementary probes for the materials in group A and C, and the ends of the DNA in group B are labeled with sulfhydryl groups.

[0023] In the second step, the mother chip is constructed with group A DNA materials.

[0024] The third step is to use group B probes to hybridize with the mother chip at 25-50° C. for half an hour, and then wash.

[0025] The fourth step is to pour the polymerization reaction solution system of acrylamide monomer and its crosslinking agent on the mother chip area...

Embodiment 3

[0030] Embodiment 3, based on the unrestricted replication of the DNA chip of thiol and polyacrylamide gel system

[0031] The first step is to prepare three sets of DNA materials A, B, and C. The A set is used to construct the mother chip. The sequence and length of the C set are the same as those of the A set except for the sulfhydryl label at the end. The sequence and length of the B set are the same as those of the A set. The materials in group A and C are complementary, and they are complementary probes for the materials in group A and C, and the ends of the DNA in group B are labeled with sulfhydryl groups.

[0032] In the second step, the mother chip is constructed with group A DNA materials.

[0033] The third step is to use group B probes to hybridize with the mother chip at 25-50° C. for half an hour, and then wash.

[0034] The fourth step is to pour the polymerization reaction solution system of acrylamide monomer and its crosslinking agent on the mother chip area...

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Abstract

This invention provides a method for reproducing chips used in computation of DNA, starting from a mother chip, the method intercrosses all the complementary DNA probes with the mother chip, in which, the chemical group connected on the copmplementary probes are reacted with the double bonds in an acrylamide monomer to be tramsferred onto the surface of the acrylamide carrier to get the mirror-image chip of a first operation result, then utilizes the probes in conformity with the DNA when setting up the mother chip to be intercrossed and cleaned on the mirror-image chip to be aged with mercapto-alcohol solution together with the polyacrylamide gel block to clean and remove dissociated mercapto-alcohol then to pour the acrylamide polymer into the polycrylamide region to be heated and the mercapto group of the intercrossed probes is reacted with the monomer double bond of the acxrylamide then to be transferred to the gel surface to form a reproduced chip same to the mother chip.

Description

technical field [0001] The invention relates to a method in the technical field of DNA chips, in particular to a chip replication method for DNA calculation. technical background [0002] Since the concept of DNA computing was proposed in 1994, it has aroused people's great interest, and it seems to see the dawn of solving the completeness problem and the multi-parameter satisfaction problem. The early DNA computing is based on the solution system, due to the reaction control of DNA in the solution and Separation is difficult, especially for multivariate computational problems. Therefore, a surface-based DNA computing method in which DNA is immobilized on the surface in advance has been developed. Surface computing has the following advantages, which makes DNA calculation relatively simple; saves the amount of DNA; the result is easy to purify; it represents the development direction of DNA computing. Existing surface DNA computing methods require multiple hybridizations, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G06F17/00G06F7/00
Inventor 欧阳振乾张治州胡钧孙洁林
Owner SHANGHAI JIAO TONG UNIV
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