Creatinine content determination method and creatinine diagnosis kit
A diagnostic kit and measurement method technology, applied in the field of medical testing and measurement, can solve problems such as slowness, inconvenient testing, and reduced accuracy of reagent testing, and achieve accurate and high-precision test results
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Embodiment 1
[0097] Embodiment one (single dose)
[0098] The creatinine diagnostic kit of the present embodiment comprises:
[0099] Buffer 80mmol / l
[0100]Coenzyme 1mmol / l
[0101] Creatinase 8000U / l
[0102] Creatinase 8000U / l
[0103] Sarcosine oxidase 6000U / l
[0104] Aldehyde dehydrogenase 8000U / l
[0105] Peroxidase 10000U / l
[0106] Stabilizer 50% (total volume)
[0107] 4-amino antiarsenic 2mmol / l
[0108] Carbolic acid 10mmol / l
[0109] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 495-505nm, test sub-wavelength above 600nm, the volume ratio of the creatinine sample to the reagent to be tested is 1 / 25, and the reaction direction is positive reaction.
[0110] After the sample and reagents are added and allowed to mix, the following reactions occur:
[0111] Creatinine + water Creatinase creatine
[0112] Creatine + water creatinase Sarcosine + urea
Embodiment 2
[0118] Embodiment two (two doses)
[0119] The creatinine diagnostic reagent of the present embodiment has:
[0120] Reagent I
[0121] Buffer 100mmol / l
[0122] Coenzyme 3mmol / l
[0123] Creatinase 14000U / l
[0124] Sarcosine oxidase 8000U / l
[0125] Aldehyde dehydrogenase 14000U / l
[0126] Peroxidase 15000U / l
[0127] Stabilizer 50% (total volume)
[0128] 4-amino antiarsenic 1mmol / l
[0129] Reagent II
[0130] Buffer 100mmol / l
[0131] Creatinase 14000U / l
[0132] Stabilizer 50% (total volume)
[0133] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 1mmol / l
[0134] When measuring creatinine content, the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 546nm, the secondary wavelength of the test is above 630nm, the volume ratio of the creatinine sample to the reagent is 1 / 25, and the reaction direction is positive reaction.
[0135] The specific measurement steps are:
[0136] Creatinine + water Creatinase ...
Embodiment 3
[0143] Embodiment three (three doses)
[0144] The creatinine diagnostic reagent of the present embodiment is three doses, has:
[0145] Reagent I
[0146] Buffer 120mmol / l
[0147] Coenzyme 5mmol / l
[0148] Stabilizer 20mmol / l
[0149] 3-methyl-2-benzothiazolone hydrazone 2mmol / l
[0150] Reagent II
[0151] Buffer 120mmol / l
[0152] Creatinase 20000U / l
[0153] Sarcosine oxidase 10000U / l
[0154] Aldehyde dehydrogenase 20000U / l
[0155] Peroxidase 20000U / l
[0156] Stabilizer 50% (total volume)
[0157] Reagent III
[0158] Buffer 120mmol / l
[0159] Creatinase 20000U / l
[0160] Stabilizer 50% (total volume)
[0161] Dimethylaniline 2mmol / l
[0162] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 578nm, test sub-wavelength above 700nm, the volume ratio of the creatinine sample to be tested is 1 / 25, and the reaction direction is positive reaction.
[0163] The specific measurement steps are:
[0164] ...
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