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Biological preparation for poisoning nematode on pine tree and its preparation method and use

A technology of pine wood nematodes and biological preparations, applied in the directions of botanical equipment and methods, biocides, applications, etc., can solve problems such as ineffectiveness of pine wood nematodes

Inactive Publication Date: 2005-02-16
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no effective pesticides and control methods for pine xylophilus at home and abroad. At present, the main conservative control measures are: eliminate the sowing medium pine beetle, cut down and burn susceptible pine trees, strengthen quarantine, and control further transmission

Method used

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  • Biological preparation for poisoning nematode on pine tree and its preparation method and use
  • Biological preparation for poisoning nematode on pine tree and its preparation method and use
  • Biological preparation for poisoning nematode on pine tree and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The mycelium of Caryospora callicarpa ZD2 was inoculated on the slant of test tube agar medium, and the medium formula was PDA medium, and cultured at 20° C. for 9 days to obtain test tube species.

[0042] The test tube seed is inoculated in the 250ml Erlenmeyer flask (70ml per bottle) liquid medium again, and the medium formula is 2% soybean meal; 2% glucose; 1% starch; 0.2% yeast extract; 0.4% sodium chloride; 0.05 % Potassium dihydrogen phosphate; 0.05% magnesium sulfate; 0.2% calcium carbonate, the rest is water, the pH is natural; cultured on a shaker at 22°C with a rotation speed of 250 rpm for 7 days.

[0043] Then put the fermented liquid containing mycelia into a centrifuge for centrifugation at a speed of 3600 rpm for 20 minutes, and filter to obtain the biological agent for killing pine wood nematodes.

Embodiment 2

[0045] The test tube culture method is the same as in Example 1.

[0046] Inoculate the test tube seed on the wheat grain solid medium, the medium formula is 1% calcium carbonate; 3% soybean flour; 0.5% ammonium sulfate; 0.5% superphosphate; wheat grain 95; natural pH, water content 60 %. Soak the wheat grains in clean water for 24 hours, pick them up and put them in boiling water to cook. It is suitable that the cooked skin is not broken. Pick up the water and mix it with auxiliary materials and put it into a 1000ml triangular flask. Each bottle is 700ml, 120 After sterilizing at ℃ for 30 minutes, culture at 22℃ for 16 days after inoculation.

[0047] Then dig out the solid-cultured bacterial strain, freeze-dry, use chloroform and methanol in a ratio of 1:1 to prepare a solvent for leaching, and concentrate after leaching to obtain the total crude extract. The crude extract is dissolved in a small amount of dimethyl sulfoxide (DMSO), and then dissolved in a certain concentr...

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Abstract

The invention relates to a biopreparation for poisoning pine nematodes and its preparing method and application. Its producing bacterial strain-Caryospora callicarpa ZD2 has a preserved number of CGMCC NO.0983. After fermented by liquid and solid, the ZD2 bacterial strain is prepared into a biopreparation with poisoning activity for pine nematodes. The invention has a good poisoning effect on the pine nematodes and a good prospect in application and development.

Description

Technical field: [0001] The invention relates to a biological agent for poisoning and killing pine wood nematodes, a preparation method and application thereof, and belongs to the technical field of biological pesticides. Background technique: [0002] The pine wood nematode (Bursaphelenchus xylophilus) is a kind of harmful organism that is extremely harmful to pine trees, and is called "pine tree cancer". Mainly distributed in the United States, Japan, Canada, China and other countries, it is a catastrophic disaster for pine trees and an important international quarantine object. Since the first discovery of pine xylophilus in the Zhongshanling forest area of ​​Nanjing in 1982 in my country, it has spread to more than 10 provinces and regions including Jiangsu, Anhui, Guangdong, Zhejiang, Shandong, Taiwan and Hong Kong. It will cause serious damage to pine forest resources, ecological environment and natural landscape, and pose a serious threat to pine forests in vast suit...

Claims

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Application Information

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IPC IPC(8): A01N63/04
Inventor 张克勤赵智娴董瑾艳周薇
Owner YUNNAN UNIV
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