Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immunogen of cycloproply thaastral and preparation process thereof

A technology of ciprofloxacin and immunogen, which is applied in the field of enzyme-linked immunosorbent assay of ciprofloxacin

Inactive Publication Date: 2005-01-05
SHANDONG UNIV
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as we know, there is no report on the synthesis of the immunogen of ciprofloxacin in the world

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunogen of cycloproply thaastral and preparation process thereof
  • Immunogen of cycloproply thaastral and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] (1) Preparation of solution A: In a 50ml circular reaction bottle, add 13.9mg (0.042mmol) of ciprofloxacin (0.042mmol), 19.33mg (0.168mmol) of hydroxysuccinic acid imide (NHS), ethyl [3 -(Dimethylamino)propyl]carbodiimide hydrochloride (EDC.HCl) 48.3mg (0.252mmol), dissolved in 10ml of dimethylformamide (DMF). React for 4 hours. Solution A is obtained. spare.

[0030] (2) Preparation of Solution B: In a 100ml round reaction bottle, add 476mg BSA and 50ml PBS (pH=7.4). Solution B is obtained. spare.

[0031] (3) Preparation of ciprofloxacin immunogen: gradually add solution A to solution B at 20° C. under stirring. React for 4 hours. A ciprofloxacin immunogen solution was obtained. Dialyze against PBS buffer for three days, changing the dialysate every 12 hours. Centrifuge at high speed (20,000g) for 30 minutes. The supernatant was freeze-dried to obtain 412 mg of white solid powder, which was the crude ciprofloxacin immunogen. Sephadex-G75 (Sigma) was used for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention is a Ciprofloxacin immunogen, coupling Ciprofloxacin with bovine serum albumin at room temperature, using PBS buffer liquor to dialyze, freeze-drying supernatant by high-speed centrifugation and obtaining Ciprofloxacin crude product, then using glucose gel chromatography for purifying, freeze-drying to obtain Ciprofloxacin pure product. The research shows it succeeds in synthesizing the immunogen able to be used in preparing Ciprofloxacin specific antibody. It lays the foundation of development of ELISA test method of Ciprofloxacin medicine residues.

Description

Technical field [0001] The invention relates to the field of detection of animal source food drug residues. related to the enzyme-linked detection method. More specifically, it relates to the production technology of detection reagents used in the enzyme-linked immunoassay method for ciprofloxacin. Background technique [0002] The following definitions apply throughout the specification and claims: [0003] BSA = bovine serum albumin (Bovine Serum Albumin) [0004] PBS=Phosphate Buffered Saline (0.01M, [0005] pH=7.4) [0006] Sephadex-G75 = dextran gel [0007] Ciprofloxacin-EDC = active intermediate formed by ciprofloxacin and carbodiimide [0008] Ciprofloxacin (Ciprofloxacin) belongs to the quinolone antibiotics. Because of its broad antibacterial spectrum and strong bactericidal ability, this kind of antibiotics is widely used in livestock and poultry aquaculture after oxytetracycline. Such drugs can effectively prevent and treat bacterial and mycoplasma ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765G01N33/531
Inventor 郗日沫卢圣欣
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com