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Compound artificial skin of double side implanted two kinds of cell and its construction method

A technology of artificial skin and construction method, applied in biochemical equipment and methods, medical science, microbiology, etc., can solve the problems of poor ability of collagen gel to resist collagen degradation, complex production process of collagen gel, and short time for culturing cells. Achieve the effects of good physical properties, good physical characteristics, and strong anti-infection ability

Inactive Publication Date: 2004-07-14
RADIOLOGY INST ACAD OF MILITARY MEDICINE SCI PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the preparation of artificial skin has continued to develop and progress. For example, the US patent US 5 282 859 discloses an artificial composite skin constructed from bovine type I and III collagen. The disadvantage is that after glutaraldehyde treatment, the remaining cells Toxic, and the strength is not enough, and the immunogenicity is strong, simply using type I and III collagen as a scaffold, and lacking the introduction of cytokines; Cultivate cells derived from mammalian skin, and then freeze-dry to produce artificial skin. The disadvantage is that ammonia is used to neutralize collagen gel, which is not suitable for cell culture. The time for culturing cells is short, and the formed cultured skin is different from natural skin in terms of tissue structure. Big difference, collagen gel has poor ability to resist collagen degradation, the skin is brittle, difficult to operate, and the production process of collagen gel is complicated

Method used

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  • Compound artificial skin of double side implanted two kinds of cell and its construction method
  • Compound artificial skin of double side implanted two kinds of cell and its construction method
  • Compound artificial skin of double side implanted two kinds of cell and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The fetal cowhide is depilated, fascia, fat and blood stains are removed according to the conventional method, cleaned, frozen, cut into 0.5mm thick slices, dehydrated with acetone three times, degreased with petroleum ether three times, and then vacuumed to remove petroleum ether to make dehydrated degreasing Fetal calf leather is used as raw material, 10g of raw material is taken, washed with double distilled water, added to the reactor, and an aqueous solution prepared by 0.1g of gastric enzyme and 100g of water is added to carry out an enzyme treatment. Under the conditions of 20°C and pH=2, mechanical Vibrate for 24 hours to degrade the covalent bonds between tissue fibers, centrifuge, discard the supernatant, take the precipitate, wash with double distilled water three times, add 500g of 0.5M acetic acid aqueous solution, and mechanically shake for 32 hours at 0°C Finally, add sodium chloride again to make the concentration of sodium chloride in the solution reach ...

Embodiment 2

[0031] The bovine Achilles tendon is dehydrated and degreased according to the method in Example 1, and petroleum ether is removed by vacuuming, and the dehydrated and degreased bovine Achilles tendon is made as a raw material, and 10 g of the raw material is taken, washed with double distilled water, and then added to the reaction vessel. The aqueous solution prepared by chymotrypsin and 100g water was subjected to an enzyme treatment, and was subjected to mechanical vibration at 30°C and pH=6 for 16 hours to degrade the covalent bonds between tissue fibers. After centrifugation, the supernatant was discarded, and the precipitate was collected. Wash with double distilled water four times, add 1000g 0.5M acetic acid aqueous solution, at 10°C, mechanically vibrate for 28 hours, then add sodium chloride to make the concentration of sodium chloride in the solution reach 2M, after the precipitation is complete, centrifuge, Discard the supernatant, take the precipitate, put it into ...

Embodiment 3

[0033] The bovine Achilles tendon dehydrated and degreased in Example 2 is used as a raw material, and 10 g of the raw material is taken, washed with double distilled water and added to a reaction vessel, and an aqueous solution prepared by 0.5 g pineapple enzyme and 100 g water is added to carry out an enzyme treatment. Under the conditions of 40°C and pH=8, mechanically vibrate for 8 hours to degrade the covalent bonds between tissue fibers, centrifuge, discard the supernatant, take the precipitate, wash four times with double distilled water, add 1500g of 0.5M acetic acid aqueous solution At 20°C, after mechanical vibration for 24 hours, add sodium chloride to make the concentration of sodium chloride in the solution reach 2M. After the precipitation is complete, centrifuge, discard the supernatant, take the precipitate, and put it into a dialysis bag for dialysis Three times, after collecting the dialysis, the collagen solution was added into the reactor, and the aqueous so...

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Abstract

The present invention relates to a kind of compound artificial skin with two kinds of cells implanted on its two sides and its construction process. On two sides of rack of ox collagen membrane with epidermal growth factor, fibroblast and epidermal cell are implanted, and the middle part is growing fibroblast of density grater than 1E6 / sq cm. The artificial skin has excellent physical characteristics, high strength, high flexibility, high infection resistance, no antigenicity, convenient operation transplantation and good fusion with peripheral skin after transplantation.

Description

technical field [0001] The invention relates to subhuman skin, in particular to a composite artificial skin implanted with fibroblasts and epidermal cells on both sides, which belongs to a medical skin wound covering. [0002] The invention also relates to the construction method of the composite artificial skin. Background of the invention [0003] Artificial skin is clinically used for transplantation of skin injuries, which can promote wound healing, reduce scar formation, and prevent infection, especially for patients with large areas of skin loss. Due to the lack of autologous skin that can be transplanted, only the use of wound coverings can solve skin loss. early and late problems. [0004] At present, the skin wound coverings in clinical application in foreign countries include: epidermal grafts, dermal grafts (such as allogeneic dermis, exfoliated dead dermis, synthetic omentum, acellular collagen sponge, etc.), and composite skin grafts, such as collagen Gel skin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61L27/60C12N5/00
Inventor 刘杰王德文崔雪梅张燕杨颖颙于淑贤
Owner RADIOLOGY INST ACAD OF MILITARY MEDICINE SCI PLA
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