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Microflow pump for combining use between capillary electrophoresis/electrochromatography and mass spectrum

A technology of capillary electrophoresis and electrochromatography, which is applied in the field of microflow pumps, can solve the problems of low detection sensitivity, inability to achieve electrospray conductivity, increase the cost of instruments, etc., and achieve the effects of wide application range, easy popularization and application, and reduced instrument cost

Inactive Publication Date: 2004-05-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In 2000, Agilent Corporation and Beckman Corporation launched their respective commercialized capillary electrophoresis / electrochromatography and mass spectrometry instruments. Since mass spectrometry is a general-purpose high-sensitivity detector, its combination It basically solves the disadvantages of low detection sensitivity of capillary electrophoresis and electrochromatography; there are many types of interface between electrophoresis / electrochromatography and mass spectrometry, of which the most widely used is the electrospray interface (ESI); due to capillary electrophoresis and capillary electrochromatography The flow rate is very small, and stable electrospray and stable conductivity cannot be achieved simply by such a small flow rate; in order to solve the above problems, ESI / MS generally needs to provide a sheath fluid, and the flow rate of the sheath fluid is generally required to be in nanoliters or microliters. In the range of liters per minute, it is difficult for ordinary high-pressure liquid phase pumps to meet the above requirements. Generally, CEC / CE-ESI / MS needs to be equipped with micro-flow pumps, which further increases the cost of the instrument

Method used

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  • Microflow pump for combining use between capillary electrophoresis/electrochromatography and mass spectrum
  • Microflow pump for combining use between capillary electrophoresis/electrochromatography and mass spectrum
  • Microflow pump for combining use between capillary electrophoresis/electrochromatography and mass spectrum

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Embodiment Construction

[0024] In order to improve the stability of the capillary electrochromatographic column, it is generally necessary to bond the stationary phase to the inner wall of the capillary. For the polymer monolithic column, most of them are realized by using a bifunctional reagent (γ-MAPS). One end of the bifunctional reagent is an alkoxy group. , which reacts with the silanol on the inner wall of the capillary; the double bond at the other end participates in the polymerization when the monomer is polymerized (see the following example for the molecular structure of the bifunctional reagent I). The stationary phase achieves indirect bonding with the inner wall of the capillary through a bifunctional reagent, which improves the stability of the bed.

[0025]

[0026] capillary monolith

monomer mixture

Porogen

BMA

EDMA

AMPS

n-propanol

1,4-Butanediol

water

a

60

59.7

0.3

60

30

...

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Abstract

The microflow pump is composed of liquid phase pump in high pressure and cascaded columns with thin internal diameter (less than 2 mm) with pad being filled for providing sheath flow liquid for capillary electrophoresis / electrochromatography and mass spectrum combination instrument. The invention lowers cost of electrophoresis / electrochromatography and mass spectrum combination instrument, increases adaptability of experimentation as well as possessing wider suitable range and it is apt to expand the application.

Description

technical field [0001] The invention relates to capillary electrophoresis / electrochromatography and mass spectrometry technology, in particular to a microflow pump for capillary electrophoresis / electrochromatography and mass spectrometry. Background technique [0002] Capillary electrophoresis is one of the most effective techniques for separating charged samples according to the difference in electrophoretic mobility of charged samples in the electric field; reversed-phase high performance liquid chromatography separates samples according to the strength of the hydrophobic interaction between the sample and the surface of the stationary phase. It is a widely used separation mode of high performance liquid chromatography; capillary electrochromatography is a micro-separation technology combining high performance liquid chromatography and capillary electrophoresis, adhering to the high selectivity of high performance liquid chromatography and the high efficiency of capillary e...

Claims

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Application Information

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IPC IPC(8): G01N30/36G01N30/72
Inventor 平贵臣飞利浦·施密特考普林张玉奎张维冰张丽华张凌怡安童尼尔斯·凯楚普
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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