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Method for preparing specific Immunoglobulin of cystvirus and adenovirus in respiratory tract

A technology of immunoglobulin and syncytial virus, applied in the field of respiratory syncytial virus and adenovirus-specific immunoglobulin preparation, can solve the problem of low immune function

Inactive Publication Date: 2004-03-31
SHANDONG BAIYI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adults generally have immunity against these two viruses, but in some patients with low immune function, such as long-term use of radiotherapy, chemotherapy patients, surgical patients, organ transplant patients, etc., it can also cause infection. It is a chemical synthetic drug, Chinese herbal medicine, and biological preparations such as interferon. Among these drugs, there is no drug that is effective against viruses and has a certain effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] According to the above technical scheme, the preparation method of high titer specific anti-respiratory syncytial virus and adenovirus immunoglobulin drug comprises the following steps:

[0019] First, prepare respiratory syncytial virus and adenovirus antigens; inoculate RSV and adenovirus into Hep-2 cells, Hala cells or human embryonic kidney cells that have grown into a single layer, incubate at 37°C, and observe the cells every day Lesions, when the cytopathic pathology (CPE) appears +++ or the virus antigen test is positive, the cells and supernatant are collected, the virus is inactivated, ultrasonically crushed, and stored in the refrigerator; or according to respiratory syncytial virus and adenovirus And gene sequence, the antigen prepared by genetic engineering method is expressed.

[0020] Secondly, the antigen was concentrated with 20% sucrose sedimentation or viral antigen was purified by discontinuous gradient density centrifugation or purified by chromatog...

Embodiment 1

[0029] Example 1: Anti-RSV and Adenovirus Specific Immunoglobulin Sprays

[0030]The antiviral specific immunoglobulin prepared according to the above method can be locally applied III and its water-soluble and stable antibiotics can be inhaled through the upper respiratory tract spray to locally neutralize the virus and resist the secondary infection of bacteria in the upper respiratory tract mucosa. role. Store frozen and use within 1 week after freezing.

Embodiment 2

[0031] Example 2: Anti-respiratory syncytial virus and adenovirus high titer specific immunoglobulin injection

[0032] The antiviral immunoglobulin prepared according to the above-mentioned method can be injected into the body through a large dose after passing the sterility test and the safety test, and can be used for the rescue of critically ill patients and patients with low immune function.

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PUM

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Abstract

A method of preparing immunoglobins with high valence and high specialty against respiratory syncytial virus and adenovirus. It comprises culturing respiratory syncytial virus and adenovirus with Hep-2 cell strain, Hala Strain, preparing virus antigen, or preparing protective antigens according to sequences of respiratory syncytial virus and adenovirus by the genetic engineering technology; then,centrifuging the prepared virus antigens and getting purified antigens, or concentrating antigens by 20úÑ sucrose, or purifying antigens by chromatographic absorbing columns, screening high-valent immunoglobins against respiratory syncytial virus and adenovirus, and neutralizing virus in viro; finally extracting immunoglobins from bloods containing high-valent immunoglobins against respiratory syncytial virus and adenovirus by a low-temperature alcohol method or ion exchanging chromatography and so on.

Description

1. Technical field [0001] The invention belongs to the fields of virology and immunology, and relates to a method for preparing medicine, in particular to a method for preparing respiratory syncytial virus and adenovirus-specific immunoglobulin for prevention and treatment. 2. Background technology [0002] Respiratory syncytial virus (Respiratory virus RSV) and adenovirus (Adenovirus Adv) are the two most common viruses that cause respiratory tract infections. Especially for upper respiratory tract infections in children, more than 50% of viral infections are caused by these two viruses, which are prevalent in winter and spring every year, and sporadic cases exist throughout the year. Adults generally have immunity against these two viruses, but in some patients with low immune function, such as long-term use of radiotherapy, chemotherapy patients, surgical patients, organ transplant patients, etc., it can also cause infection. It is a synthetic chemical drug, Chinese herb...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/18G01N33/569G01N33/68
Inventor 张国成许东亮余军
Owner SHANDONG BAIYI PHARMA
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