Method for preparing long acting progestational hormone injection embedded agent and use
A technology of progesterone and preparation, which is applied in the field of preparation of long-acting progesterone injection implants, which can solve the problems of inconvenience to patients, liver and kidney damage, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0022] Take 20mg gestrinone, 80mg polylactide-glycolide (lactide:glycolide=75:25), dissolve in 3ml dichloromethane, and drop it into 100ml under vigorous stirring (1000rpm) In 0.2% sodium carboxymethylcellulose aqueous solution, continue to stir for 5-10 minutes after dripping, then reduce the stirring speed to 300rpm, evaporate at 25°C for about 12h, centrifuge, pour off the supernatant, filter the precipitate under reduced pressure, and a small amount of distilled water Wash the precipitate, dry under reduced pressure at room temperature, and the diameter of the microspheres is 40-120 μm.
Embodiment 2
[0024] Take 20mg gestrinone and 80mg polylactic acid, dissolve them in 1ml dichloromethane, add 5ml 0.5% PVA aqueous solution to the high-speed homogenizer, stir vigorously at 6000rpm for 2min, pour into 100ml 0.5% PVA aqueous solution, and Evaporate at 300 rpm at 25°C for about 12 hours, centrifuge, pour off the supernatant, filter the precipitate under reduced pressure, rinse the precipitate with a small amount of distilled water, and dry under reduced pressure at room temperature. The diameter of the microspheres is 30-80 μm.
Embodiment 3
[0026] Inhibition test of rat estrous cycle:
[0027] Method: The rats with regular estrous cycle were divided into two groups, and the following corresponding injections were given on the day when the pictures of the vaginal secretions of the rats showed anaphase (white blood cells):
[0028] Negative control group: subcutaneous (s.c.) injection of vehicle (n=5).
[0029] The microsphere group of Example 1: single dose s.c. injection of microspheres with a release rate of 0.5 mg / week / kg, a particle size of 40-120 μm, and a gestrinone content of 25% in the microspheres (n=5).
[0030] After injection, regularly observe and record vaginal cell smears every day. Rat vaginal smears continue to show white blood cells, indicating that the estrous cycle is inhibited. If keratinocytes appear, it indicates that the estrous cycle begins to recover. The vaginal smear method can be used to judge the drug effect of the preparation. time.
[0031] Results: After s.c. injection of a singl...
PUM
Property | Measurement | Unit |
---|---|---|
diameter | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com