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Target directional DNA vaccine for preventing caries and preparation method thereof

A DNA vaccine, anti-caries technology, applied in recombinant DNA technology, DNA/RNA fragments, pharmaceutical formulations, etc., can solve problems such as non-compliance with DNA vaccine requirements

Inactive Publication Date: 2006-11-15
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, the vector pCI plasmid of the vaccine contains the ampicillin resistance gene, which does not meet the relevant requirements for the vector backbone of DNA vaccines

Method used

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  • Target directional DNA vaccine for preventing caries and preparation method thereof
  • Target directional DNA vaccine for preventing caries and preparation method thereof
  • Target directional DNA vaccine for preventing caries and preparation method thereof

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Experimental program
Comparison scheme
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Embodiment Construction

[0050] The present invention will be described in further detail below according to accompanying drawing:

[0051] according to figure 1 It can be seen that the professional molecular biology software: DNASIS comprehensive DNA sequence analysis software (Hitachi, Japan), Gene Construction kit 2.0 gene simulation cloning software (Textco, USA) was used to analyze and design the targeted anti-caries DNA vaccine and simulate the whole process of cloning.

[0052] according to figure 2 It can be seen that the targeted anti-caries eukaryotic expression plasmid pGJGLU (patent application number: 02139123.8) and pVAX1 vector are treated with restriction enzymes NheI and NotI; the targeted fusion gene (including CTLA-4-Ig target gene, GLU) is excised and NheI and NotI enzyme-digested pVAX1 vector fragments, gel recovery and purification of the target fragments; the above two purified target fragments at T 4 Ligate overnight at 20-25°C under the action of DNA ligase, transform comp...

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Abstract

The invention discloses a targeted anti-caries DNA vaccine and a preparation method thereof, Escherichia coli JM109 / pGJGLU / VAX, CCTCC NO: M204027. The targeted anti-caries eukaryotic expression plasmid pGJGLU was digested with restriction enzymes NheI and NotI to excise the targeted fusion gene (including CTLA-4-Ig target gene and GLU fragment), and the targeted fusion gene was cloned into the pVAX1 vector, Enzyme digestion confirmed the correctness of the construction, and named it pGJGLU / VAX, transfected eukaryotic CHO cell line, and confirmed that the recombinant protein could be correctly expressed in eukaryotic cells. The method of the invention is simple, high in safety, low in production cost, durable in immune response, and can greatly improve its immune efficacy.

Description

technical field [0001] The invention belongs to the technical field of oral preventive medicine, and more specifically relates to a sequence encoding human cytotoxic T lymphocyte antigen 4 signal peptide, extracellular region and Igγ 1 A DNA anti-caries vaccine of hinge region, constant region fusion gene and mutans glucosyltransferase gene, and the present invention also relates to a preparation method of the targeted anti-caries DNA vaccine. Background technique [0002] Dental caries is a common and frequently-occurring disease that seriously endangers the health of human oral cavity and the whole body. The loss of function, inflammation, pain, and organ diseases caused by dental caries can seriously affect the quality of life, reduce the health quality of the population, and bring great harm to society. Caries is a bacterial infectious disease, and Streptococcus mutans (hereinafter referred to as Streptococcus mutans) is the main cariogenic b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00C12N15/62A61P1/02
Inventor 樊明文贾荣郭继华边专陈智彭彬范兵
Owner WUHAN UNIV
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