Method and apparatus for automatic updating solid phase carrier in minisize reactor

Abstract

The invention relates to an automatic renewal method and device for a solid-phase carrier in a microreactor. The feature of the device is that an inflow channel and an outflow channel for the solid-phase carrier are respectively arranged at both ends of the microreactor, and the upper part of the inflow channel is connected with the solid-phase carrier. The two ends of the container and the microreactor are equipped with plungers, the plungers are provided with passages for circulating samples and buffer solutions, and the tops of the upper and lower plungers are provided with filter screens. The steps of the method are: the solid-phase carrier is stored in the buffer, so that the liquid level of the buffer is higher than the solid-phase carrier; the inflow channel and the outflow channel are opened and cut off by the upper and lower plungers, and the three modes of automatic update of the solid-phase carrier are completed: update mode, Test mode, flush mode. The beneficial effect of the invention is that the solid-phase carrier is used once, the reaction surface does not need to be regenerated, the detection accuracy is improved, the detection time is saved, the renewal of the solid-phase carrier is realized automatically, and external interference factors are reduced.

Description

technical field [0001] The invention relates to an automatic renewal method and device for solid-phase carriers in microreactors, which can be applied in various analysis fields involving liquid-solid two-phase chemical reactions. Background technique [0002] The development of science requires that the field of analysis can provide real-time, accurate and comprehensive information with lower consumption, simpler methods and equipment, and faster analysis speed. The obvious development trend is the miniaturization, integration and automation of analysis equipment . Chemical reactions based on liquid-solid two-phase are widely used in the field of analysis. Usually, various functional groups (such as enzymes, cells) are immobilized on solid phase supports, such as resins, porous glass, etc., to improve stability and repeated use. , and the reaction is controlled by liquid phases such as sample and buffer. The solid phase is generally regarded as a fixed component of the sy...

AI Technical Summary

Problems solved by technology

The traditional method of updating the solid-phase carrier in the microreactor is manual filling, which has at least the following disadvantages, which is time-consuming and labor-intensive; it is easy to introduce air bubbles and cause interference; it will destroy the stable state that the test has reached, thereby prolonging the detection time

For example, the calorimetric immobilized enzyme biosensor needs about half an hour to stabilize the baseline before testing; in addition, if the microreactor and the solid-phase carrier are designed as one, it must be updated and discarded together with the solid-phase carrier, so that increase costs, pollute the environment

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