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Method for constructing characteristic chromatogram of caulis sinomenii

A construction method and feature map technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of complicated operation, inability to distinguish the basis, and low detection efficiency, and achieve the effect of convenient operation, efficient method and wide application.

Active Publication Date: 2022-08-05
JIANGYIN TIANJIANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: In view of the shortcomings of the existing Qingfengteng quality control methods, such as inability to distinguish the base, limited quality control components, complicated operations, and low detection efficiency, the present invention aims to provide an efficient and reliable Qingfengteng that can identify the base. Method for constructing the characteristic map of Fengteng

Method used

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  • Method for constructing characteristic chromatogram of caulis sinomenii
  • Method for constructing characteristic chromatogram of caulis sinomenii
  • Method for constructing characteristic chromatogram of caulis sinomenii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: Construction of characteristic map of Qingfengteng (Qingteng) standard decoction

[0062] 1. Preparation of reference solution

[0063] Take about 0.4 g of the reference medicinal material of C. chinensis, put it in a conical flask with a stopper, add 25 ml of water, decoct for 30 minutes, let it cool, shake well, filter, and take the filtrate as the reference medicinal material solution. In addition, take appropriate amounts of sinomenine reference substance, magnoflorine reference substance, and syringaresinol diglucoside reference substance, accurately weigh them, and add methanol to make 0.3 mg of sinomenine, 50 μg of magnoflorine, and syringaresinol per 1 ml respectively. A solution of 50 μg of diglucoside was used as a reference solution.

[0064] 2. Preparation of the test solution

[0065] Take an appropriate amount of the standard decoction of Qingfengteng (Qingteng) and grind it finely. Take about 0.1g, accurately weigh it, put it in a stopper...

Embodiment 2

[0073] Embodiment 2: the investigation of test sample pretreatment extraction solvent

[0074] 1. Preparation of reference solution

[0075] The relevant method is the same as that in Example 1.

[0076] 2. Preparation of the test solution

[0077] Take about 0.1 g of the standard decoction of Qingfengteng (Qingteng), 5 parallel parts, accurately weighed, put in a stoppered conical flask, and accurately add water, 30% ethanol, 50% ethanol, 70% ethanol, and 95% ethanol. 25ml of ethanol, weighed, ultrasonically treated (power 250W, frequency 40kHz) for 30 minutes, allowed to cool, weighed again, used the corresponding solvent to make up the lost weight, shake well, filter, and take the filtrate.

[0078] 3. Construction of feature map

[0079] (1) Chromatographic conditions

[0080] The relevant method is the same as that in Example 1.

[0081] (2) Testing and results

[0082] Precisely draw 1 μL of each test solution and reference solution, inject them into a liquid chrom...

Embodiment 3

[0086] Embodiment 3: Investigation of the characteristic map of multiple batches of Qingfengteng (Qingteng) standard decoction

[0087] 1. Preparation of reference solution

[0088] The relevant method is the same as that in Example 1.

[0089] 2. Preparation of the test solution

[0090] The relevant method is the same as that in Example 1.

[0091] 3. Construction of feature map

[0092] (1) Chromatographic conditions

[0093] The relevant method is the same as that in Example 1.

[0094] (2) Testing and results

[0095]Precisely draw 1 μL of each test solution and reference solution, inject them into a liquid chromatograph, and then import the spectral data into the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2012 Edition)" recommended by the State Pharmacopoeia Committee for result analysis. Peak No. 3 (referred to as sinomenine by the reference substance) is the S1 peak, calculate the relative retention time of peak 1, peak 2 and the ...

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Abstract

The invention discloses a method for constructing a characteristic chromatogram of caulis sinomenii, which is characterized in that sinomenine, magnoflorine and clove resinol diglucoside are taken as characteristic components, common peaks of different original caulis sinomenii samples are confirmed by an efficient detection method, and the characteristic chromatogram is constructed. According to the method, samples with different basic sources can be distinguished, and alkaloid and lignan contained in the caulis sinomenii are taken as representative components, so that the product quality can be relatively comprehensively reflected; the method is suitable for rapid and comprehensive quality control of caulis sinomenii medicinal materials, decoction pieces, extracts, standard decoction, formula granules and standard samples, preparation intermediate products and finished products of classic famous prescriptions containing caulis sinomenii, and is efficient, convenient to operate and suitable for quality monitoring of continuous production.

Description

technical field [0001] The invention relates to a method for constructing a characteristic map of C. chinensis, in particular to a method for constructing an efficient and reliable characteristic map of C. chinensis. Background technique [0002] Qingfengteng is a traditional traditional Chinese medicine, and the Chinese Pharmacopoeia records Qingfengteng as the fangzhi plant Sinomeniumacutum (Thunb.) Rehd.et Wils. and Mao Qingteng Sinomeniumacutum (Thunh.) Rehd.etWils.var.cinereumRehd.et Wils. Dried cane has the functions of dispelling rheumatism, clearing meridians, and urination. It is clinically used to treat rheumatic arthralgia, joint swelling, paralysis and itching. Qingteng contains alkaloids, sterols, terpenes and other chemical components, which have a variety of pharmacological effects on the immune system, cardiovascular system and nervous system. Phenomenine and magnolanine, and less control for other components in Cinnamon. [0003] It is impossible to compre...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/74
CPCG01N30/02G01N30/06G01N30/54G01N30/74Y02A50/30
Inventor 张云天顾超翟燕娟陈盛君李松张宇静顾芹英周海琴何雪霞
Owner JIANGYIN TIANJIANG PHARMA
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