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Mortierella alpina and application thereof

A technology of Mortierella alpina and strains, applied in the fields of application, chemicals for biological control, biocides, etc.

Active Publication Date: 2022-08-05
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the application of Mortierella alpina in saline soil at present. The present invention isolates and cultivates a strain of Mortierella alpina from the wheat rhizosphere saline soil of the Yellow River Delta Research Center of the Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences. ( Mortierella alpina HSJ001), the Mortierella agent can effectively improve the activity of carbon, nitrogen and phosphorus-related enzymes in saline soil, play a certain role in the activation of carbon, nitrogen and phosphorus nutrients, and also make the roots of plants secrete a large amount of abscisic acid, promote plant nutrient absorption, improve Improve the ability of plants to cope with salt stress, which is conducive to the growth of plants in saline soil environment

Method used

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  • Mortierella alpina and application thereof
  • Mortierella alpina and application thereof
  • Mortierella alpina and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] A strain of Mortierella alpine ( Mortierella alpina HSJ001), the hyphae of this strain were observed under a microscope to form a tube, the hyphae were developed, and the growth was dense, and the hyphae were in the shape of lotus nodes and showed a clear transparent circle in the inorganic phosphorus medium. The strain has been preserved in the preservation unit designated by the State Intellectual Property Office, and the preservation date is November 30, 2021. The name of the preservation unit is: General Microbiology Center of China Microbial Culture Collection Administration Committee, address: No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, Institute of Microbiology, Chinese Academy of Sciences, deposit number: CGMCC No. 23826. The surface soil nutrient indicators are: soil salt content 2.14g / kg, pH 8.16, electrical conductivity 1.00ds / m, soil organic carbon content 7.13 g / kg, soil total phosphorus content 0.73 g / kg.

[0022] Implementation steps:

...

Embodiment 2

[0032] Implementation steps:

[0033] Step 1: Inoculate the well-grown Mortierella alpina species into PD liquid medium, and cultivate at 27 °C and 180 rpm in a shaker for 7 to 10 days, until the liquid medium is turbid and covered with a large number of mycelial balls.

[0034] Step 2: Filter the liquid cultured Mortierella alpina under sterile conditions, and rinse the filtered mycelium with sterile deionized water.

[0035] Step 3: Inoculate the clean mycelium into the sterilized wheat bran-soybean cake flour-flour medium, and keep it at 27°C for dark cultivation until the mycelium completely covers the medium.

[0036] Step 4: The wheat bran-soybean cake flour-flour culture medium of the mycelium in different triangular flasks is smashed and taken out, and the inoculum is obtained after mixing.

[0037] Step 5: Accurately weigh 3 g of the inoculum, add it to 1.5 kg of soil, mix well and then put it into a pot.

[0038] Step 6: Plant crops after adequate watering.

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Abstract

The invention discloses mortierella alpina and application thereof, the mortierella alpina is preserved in a preservation unit specified by the State Intellectual Property Office, the preservation date is November 30, 2021, the name of the preservation unit is China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.23826. The mortierella alpina can be stably propagated in soil, and can form a stable synergistic relationship with indigenous microorganisms. The mortierella alpina is derived from typical salinized soil, and is green, environment-friendly, safe and high in quality. The mortierella alpina is simple in culture process, low in preparation cost and beneficial to popularization.

Description

technical field [0001] The invention belongs to the field of microorganism Mortierella alpine, in particular to a Mortierella alpine strain and application thereof. Background technique [0002] The fungi in the soil are mainly distributed in the soil surface, and in the soil microbial taxa, they are the main force for decomposing organic matter. Some beneficial fungi in the soil promote the mutual transformation of soil nutrients by stimulating the secretion of crop growth hormones, improving the absorption of nutrients by crops and improving stress resistance and promoting crop growth. [0003] Mortierella alpine ( Mortierella alpina ) is a filamentous fungus belonging to the genus Mortierella of the order Zygomycetes Mucor, Mortierella, and is widely distributed in soil, feces and other decaying organic matter. Since it belongs to the genus Mortierella ( Mortierella ) can accumulate a considerable proportion of arachidonic acid (Arachidonic Acid, ARA or AA for short...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C05G3/00A01N63/30C09K17/14A01P21/00C09K101/00C12R1/645
CPCC12N1/14C05F11/08C05G3/00A01N63/30C09K17/14C09K2101/00
Inventor 陈林张佳宝李含放赵炳梓
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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