Preparation of bionic MOF (Metal Organic Framework) nanocapsule capable of rapidly degrading ethanol and application of bionic MOF nanocapsule in treatment of alcoholism

A nanocapsule, rapid degradation technology, applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, antidote, etc., can solve problems such as alcoholism, achieve low cost, easy large-scale production and operation simple method effect

Pending Publication Date: 2022-08-05
SHANDONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The loading of alcohol oxidase and acetaldehyde dehydrogenase can make AA@MP nanocapsules quickly complete the degradation of alcohol, and fundamentally solve the problem of alcoholism

Method used

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  • Preparation of bionic MOF (Metal Organic Framework) nanocapsule capable of rapidly degrading ethanol and application of bionic MOF nanocapsule in treatment of alcoholism
  • Preparation of bionic MOF (Metal Organic Framework) nanocapsule capable of rapidly degrading ethanol and application of bionic MOF nanocapsule in treatment of alcoholism
  • Preparation of bionic MOF (Metal Organic Framework) nanocapsule capable of rapidly degrading ethanol and application of bionic MOF nanocapsule in treatment of alcoholism

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preparation example Construction

[0038] A preparation method of biomimetic MOF nanocapsules for rapidly degrading ethanol, comprising:

[0039] will NH 2 -MIL-101(Fe) powder was dispersed in DMF solution containing hydrochloric acid, heated for reaction, solid-liquid separation, collected solid, washed and dried to obtain MOF-M;

[0040] Disperse the MOF-M in PBS, add a glutaraldehyde solution, and carry out a reaction to obtain a GA-MOF-M suspension;

[0041] Dissolve alcohol oxidase and aldehyde dehydrogenase in the GA-MOF-M suspension, stir overnight in an ice bath, separate solid-liquid, collect the precipitate and disperse the precipitate in PBS to obtain AA@MOF -M suspension;

[0042] Thiol PEG Carboxyl was added to the AA@MOF-M suspension, stirred overnight in an ice bath, and the solid was collected by centrifugation.

[0043] In some embodiments, NH 2 -The dosage ratio of MIL-101(Fe) powder and DMF solution is 30~40mg:10~15mL.

[0044] In some embodiments, the specific conditions of heating to c...

Embodiment 1

[0050] Material: Ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 2-aminoterephthalic acid (NH 2 -H 2 BDC), alcohol oxidase (AOx), acetaldehyde dehydrogenase (ALDH), sulfhydryl-PEG-carboxyl (Mw=3500Da), rhodamine B isothiocyanate (RBITC), fluorescein isothiocyanate (FITC) ), nicotinamide adenine dinucleotide (NAD + ), 3-methyl-2-benzothiazolinone hydrazine (MBTH), Cell Counting Kit-8 (CCK-8) cell counting reagents were purchased from Sigma-Aldrich. Dimethyl sulfoxide (DMSO), hydrochloric acid (HCl), acetic acid, glutaraldehyde (GA), N,N-dimethylformamide (DMF), and absolute ethanol were purchased from Sinopharm Group Pharmaceutical Co., Ltd. Ethanol detection kit was purchased from Suzhou Keming Biotechnology Co., Ltd., hydrogen peroxide (H 2 O 2 ) detection kit and malondialdehyde (MDA) detection kit were purchased from Nanjing Jiancheng Bioengineering Institute. Bicinchoninic acid (BCA) protein detection kit, triglyceride (TG) detection kit, superoxide dismutase (SOD) de...

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Abstract

The invention belongs to the field of bionic nanocapsules, and relates to preparation of a bionic MOF nanocapsule capable of rapidly degrading ethanol and application of the bionic MOF nanocapsule in treatment of alcoholism, which comprises the following steps: developing a mesoporous MOF material NH2-MIL-101 (Fe)-M, wrapping ethanol oxidase (AOx) and acetaldehyde dehydrogenase (ALDH) capable of degrading ethanol by using the mesoporous MOF material, and coupling a polyethylene glycol (PEG) shell on the outer layer of the mesoporous MOF material to prepare the bionic MOF nanocapsule capable of rapidly degrading ethanol. And preparing to obtain the bionic nanocapsule AA (at) MP. The AA (at) MP nanocapsule can quickly degrade alcohol through loading of ethanol oxidase and acetaldehyde dehydrogenase, so that the problem of alcoholism is fundamentally solved. The nanocapsule can complete rapid degradation of ethanol and reduce diffusion of harmful substances to the maximum extent, and a new strategy is believed to be provided for treatment of alcoholism.

Description

technical field [0001] The invention belongs to the field of biomimetic nanocapsules, and mainly relates to the preparation of a biomimetic MOF nanocapsule capable of rapidly degrading ethanol and its application in the treatment of alcoholism. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Alcohol is a common drink in human daily life, but excessive drinking can lead to a series of diseases, such as acute alcohol intoxication (AAI), alcoholic liver damage (ALD) and gastrointestinal diseases. According to the World Health Organization, alcohol kills 6.2% of men and 1.1% of women. In addition, alcohol is another major human disease, one of the causes of cancer, and has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/59A61K47/60A61K38/44A61P1/16A61P39/02
CPCA61K47/59A61K47/6925A61K47/60A61K38/443A61K38/44C12Y101/03013C12Y102/0101A61P1/16A61P39/02A61K2300/00Y02E50/10
Inventor 李永强耿旭东杜炫呈
Owner SHANDONG UNIV
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